Efficient Simultaneous Isolation of Pinostrobin and Panduratin A from Boesenbergia rotunda Using Centrifugal Partition Chromatography.

IF 4.2 2区化学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecules Pub Date : 2024-11-02 DOI:10.3390/molecules29215186

Wanna Eiamart, Supeecha Wittayalertpanya, Sarin Tadtong, Weerasak Samee

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Abstract

The bioactive flavonoids pinostrobin (PN) and panduratin A (PA) from Boesenbergia rotunda are essential for research and therapeutic applications. This study introduces an innovative method utilizing ultrasound-assisted extraction with n-hexane pre-treatment, followed by one-step centrifugal partition chromatography (CPC) purification. Extraction efficiency was evaluated using ultra high-performance liquid chromatography (UHPLC), and the isolated compounds were characterized through ¹H-NMR and liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS), adhering to AOAC validation guidelines. Optimal extraction conditions comprised a particle size of 125 μm, a solid-to-liquid ratio of 1:30 g/mL, and a 10 min extraction time, yielding a crude extract of 6.96 ± 0.07%. Using an n-hexane/MeOH/water (5/3.4/1.6, v/v) solvent system in ascending mode, PN (2.16 mg, 98.78% purity) and PA (0.4 mg, 99.69% purity) were isolated from 67 mg of crude extract within 30 min. This streamlined approach enhances purification efficiency, allowing for faster extraction and higher purity, making it a suitable method for commercial applications.

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利用离心分离色谱法从苧麻中高效地同时分离出 Pinostrobin 和 Panduratin A。

从罗汉果中提取的生物活性黄酮类化合物pinostrobin (PN)和panduratin A (PA)对研究和治疗应用至关重要。本研究介绍了一种创新方法，即利用超声辅助萃取和正己烷预处理，然后进行一步式离心分离色谱（CPC）纯化。使用超高效液相色谱法（UHPLC）评估了萃取效率，并根据 AOAC 验证指南，通过 1H-NMR 和液相色谱电喷雾串联质谱法（LC-ESI-MS/MS）对分离出的化合物进行了表征。最佳萃取条件包括粒度为 125 μm、固液比为 1:30 g/mL、萃取时间为 10 分钟，粗萃取率为 6.96 ± 0.07%。使用正己烷/MeOH/水（5/3.4/1.6，v/v）溶剂系统，在升序模式下，30 分钟内从 67 毫克粗提取物中分离出 PN（2.16 毫克，纯度 98.78%）和 PA（0.4 毫克，纯度 99.69%）。这种简化方法提高了纯化效率，萃取速度更快，纯度更高，适合商业应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecules 化学-有机化学

CiteScore

7.40

自引率

8.70%

发文量

7524

审稿时长

1.4 months

期刊介绍： Molecules (ISSN 1420-3049, CODEN: MOLEFW) is an open access journal of synthetic organic chemistry and natural product chemistry. All articles are peer-reviewed and published continously upon acceptance. Molecules is published by MDPI, Basel, Switzerland. Our aim is to encourage chemists to publish as much as possible their experimental detail, particularly synthetic procedures and characterization information. There is no restriction on the length of the experimental section. In addition, availability of compound samples is published and considered as important information. Authors are encouraged to register or deposit their chemical samples through the non-profit international organization Molecular Diversity Preservation International (MDPI). Molecules has been launched in 1996 to preserve and exploit molecular diversity of both, chemical information and chemical substances.