Phytoecdysteroids: Quantification in Selected Plant Species and Evaluation of Some Effects on Gastric Smooth Muscles.

IF 4.2 2区化学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecules Pub Date : 2024-10-31 DOI:10.3390/molecules29215145

Velislava Todorova, Stanislava Ivanova, Viktor Yotov, Ekaterina Zaytseva, Raina Ardasheva, Valentin Turiyski, Natalia Prissadova, Kalin Ivanov

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Abstract

Phytoecdysteroids (PEs) are naturally occurring steroid compounds, that have recently gained significant attention, due to their diverse biological activities and high therapeutic potential. The aim of the present study was to quantify some PEs including 20-hydroxyecdysterone (20-HE), ponasterone A (PA), and turkesterone (TU) in selected plant foods and Rhaponticum carthamoides extract. Furthermore, the effects of 20-HE, TU, and R. carthamoides extract, were investigated with in vitro methods using isolated smooth muscle tissues. The levels of 20-HE in the analyzed samples exhibited significant differences, with kaniwa seed extract containing the highest amount, followed by spinach leaf extract, quinoa seed extract, and asparagus stem extract. The in vitro analyses suggested that R. carthamoides extract exhibits dose-dependent cytotoxic effects on smooth muscle cells, with low doses promoting contraction and higher doses inducing relaxation. Additionally, the extract demonstrated a significant inhibitory effect on ACh-induced contractions, while 20-HE enhanced the contractile response. The current findings highlighted phytoecdysteroids' potential for modifying gastrointestinal motility.

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植物蜕皮激素：选定植物物种的定量以及对胃平滑肌某些影响的评估。

植物蜕皮甾类化合物（PEs）是一种天然存在的甾类化合物，由于其具有多种生物活性和很高的治疗潜力，最近受到了广泛关注。本研究的目的是定量检测部分植物性食物和萝藦科植物提取物中的一些 PEs，包括 20-羟基蜕皮甾酮（20-HE）、芍药甾酮 A（PA）和土鳖甾酮（TU）。此外，还利用离体平滑肌组织，通过体外方法研究了 20-HE、TU 和萝藦提取物的作用。分析样本中的 20-HE 含量存在显著差异，其中卡尼娃种子提取物的含量最高，其次是菠菜叶提取物、藜种子提取物和芦笋茎提取物。体外分析表明，R. carthamoides 提取物对平滑肌细胞具有剂量依赖性的细胞毒性作用，低剂量可促进收缩，高剂量可引起松弛。此外，该提取物对 ACh 诱导的收缩有显著的抑制作用，而 20-HE 则增强了收缩反应。目前的研究结果凸显了植物蜕皮甾类化合物改变胃肠道运动的潜力。

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来源期刊

Molecules 化学-有机化学

CiteScore

7.40

自引率

8.70%

发文量

7524

审稿时长

1.4 months

期刊介绍： Molecules (ISSN 1420-3049, CODEN: MOLEFW) is an open access journal of synthetic organic chemistry and natural product chemistry. All articles are peer-reviewed and published continously upon acceptance. Molecules is published by MDPI, Basel, Switzerland. Our aim is to encourage chemists to publish as much as possible their experimental detail, particularly synthetic procedures and characterization information. There is no restriction on the length of the experimental section. In addition, availability of compound samples is published and considered as important information. Authors are encouraged to register or deposit their chemical samples through the non-profit international organization Molecular Diversity Preservation International (MDPI). Molecules has been launched in 1996 to preserve and exploit molecular diversity of both, chemical information and chemical substances.