Stem bark ethanolic extract of Pinus merkusii induces caspase 9-mediated apoptosis in HeLa cells.

IF 0.9 Q3 VETERINARY SCIENCES Open Veterinary Journal Pub Date : 2024-10-01 Epub Date: 2024-10-31 DOI:10.5455/OVJ.2024.v14.i10.12

Agung Budianto Achmad, Annise Proboningrat, Arif Nur Muhammad Ansori, Amaq Fadholly, Siti Eliana Rochmi, Rinza Rahmawati Samsudin, Nanik Hidayatik, Gracia Angelina Hendarti, Shara Jayanti

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Abstract

Background: Cervical cancer is a severe concern for women throughout the world. This percentage of cancer incidence causes sufferers to die at a high rate. It is believed that the bark of the Pinus merkusii tree contains anti-cancer compounds that inhibit cervical cancer cell growth.

Aim: This present study aims to examine the cytotoxic ability of P. merkusii tree bark ethanol extract (PMBE) by inducing apoptosis in HeLa cells.

Methods: We administered the PMBE at concentrations of 50, 100, 200, and 400 µg/ml to HeLa cell cultures. We then conducted the MTT cytotoxicity assay, detected apoptosis via Annexin V binding, and observed caspase 9 expression via immunocytochemistry.

Results: PMBE showed cytotoxic activity on HeLa cells with an IC₅₀ of 226.6 µg/ml for 24 hours of treatment. PMBE caused early apoptosis in up to 81.31% of HeLa cells, as well as increased caspase-9 expression.

Conclusion: Based on this study, PMBE is predicted to have dose-dependent antiproliferative or cytotoxicity effects on the HeLa cell line through the intrinsic pathway apoptosis mechanism.

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欧洲赤松的茎皮乙醇提取物能诱导 HeLa 细胞中由 caspase 9 介导的细胞凋亡。

背景：宫颈癌是全世界妇女严重关切的问题。宫颈癌的发病率很高，导致患者死亡率很高。目的：本研究旨在通过诱导 HeLa 细胞凋亡，检测欧洲赤松树皮乙醇提取物（PMBE）的细胞毒性能力：方法：我们将浓度为 50、100、200 和 400 µg/ml 的 PMBE 加入 HeLa 细胞培养物中。然后我们进行了 MTT 细胞毒性试验，通过 Annexin V 结合检测细胞凋亡，并通过免疫细胞化学观察 caspase 9 的表达：结果：PMBE 对 HeLa 细胞具有细胞毒性，处理 24 小时的 IC50 值为 226.6 µg/ml。结果：PMBE 对 HeLa 细胞具有细胞毒性活性，处理 24 小时的 IC50 为 226.6 µg/ml, PMBE 可导致高达 81.31% 的 HeLa 细胞早期凋亡，并增加 caspase-9 的表达：根据这项研究，PMBE 可通过内在途径凋亡机制对 HeLa 细胞株产生剂量依赖性的抗增殖或细胞毒性作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Open Veterinary Journal VETERINARY SCIENCES-

CiteScore

1.40

自引率

0.00%

发文量

112

审稿时长

12 weeks

期刊介绍： Open Veterinary Journal is a peer-reviewed international open access online and printed journal that publishes high-quality original research articles. reviews, short communications and case reports dedicated to all aspects of veterinary sciences and its related subjects. Research areas include the following: Infectious diseases of zoonotic/food-borne importance, applied biochemistry, parasitology, endocrinology, microbiology, immunology, pathology, pharmacology, physiology, epidemiology, molecular biology, immunogenetics, surgery, ophthalmology, dermatology, oncology and animal reproduction. All papers are peer-reviewed. Moreover, with the presence of well-qualified group of international referees, the process of publication will be done meticulously and to the highest standards.