Highly Porous 3D Nanofibrous Scaffold of Polylactic Acid/Polyethylene Glycol/Calcium Phosphate for Bone Regeneration by a Two-Step Solution Blow Spinning (SBS) Facile Route.

IF 4.7 3区工程技术 Q1 POLYMER SCIENCE Polymers Pub Date : 2024-10-29 DOI:10.3390/polym16213041

Vanderlane Cavalcanti da Silva, Déborah Dos Santos Gomes, Eudes Leonan Gomes de Medeiros, Adillys Marcelo da Cunha Santos, Isabela Lemos de Lima, Taciane Pedrosa Rosa, Flaviana Soares Rocha, Leticia de Souza Castro Filice, Gelmires de Araújo Neves, Romualdo Rodrigues Menezes

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Abstract

This work presents the successful production of highly porous 3D nanofibrous hybrid scaffolds of polylactic acid (PLA)/polyethylene glycol (PEG) blends with the incorporation of calcium phosphate (CaP) bioceramics by a facile two-step process using the solution blow spinning (SBS) technique. CaP nanofibers were obtained at two calcium/phosphorus (Ca/P) ratios, 1.67 and 1.1, by SBS and calcination at 1000 °C. They were incorporated in PLA/PEG blends by SBS at 10 and 20 wt% to form 3D hybrid cotton-wool-like scaffolds. Morphological analysis showed that the fibrous scaffolds obtained had a randomly interconnected and highly porous structure. Also, the mean fiber diameter ranged from 408 ± 141 nm to 893 ± 496 nm. Apatite deposited considerably within 14 days in a simulated body fluid (SBF) test for hybrid scaffolds containing a mix of hydroxyapatite (HAp) and tri-calcium phosphate-β (β-TCP) phases. The scaffolds with 20 wt% CaP and a Ca/P ration of 1.1 showed better in vitro bioactivity to induce calcium mineralization for bone regeneration. Cellular tests evidenced that the developed scaffolds can support the osteogenic differentiation and proliferation of pre-osteoblastic MC3T3-E1 cells into mature osteoblasts. The results showed that the developed 3D scaffolds have potential applications for bone tissue engineering.

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采用两步法溶液吹塑纺丝 (SBS) 简易工艺制备用于骨再生的聚乳酸/聚乙二醇/磷酸钙高孔三维纳米纤维支架。

本研究采用溶液吹塑纺丝（SBS）技术，通过简便的两步法，成功制备出加入磷酸钙（CaP）生物陶瓷的聚乳酸（PLA）/聚乙二醇（PEG）混合物高多孔三维纳米纤维杂化支架。通过 SBS 和 1000 °C 煅烧，以 1.67 和 1.1 两种钙/磷（Ca/P）比获得了 CaP 纳米纤维。通过 SBS 将 10 和 20 wt% 的 CaP 纳米纤维加入聚乳酸/聚乙二醇（PLA/PEG）混合物中，形成三维混合棉絮状支架。形态学分析表明，获得的纤维支架具有随机互连的高多孔结构。此外，纤维的平均直径从 408 ± 141 nm 到 893 ± 496 nm 不等。在模拟体液（SBF）测试中，含有羟基磷灰石（HAp）和磷酸三钙-β（β-TCP）混合相的混合支架在 14 天内沉积了大量磷灰石。CaP 含量为 20 wt%、Ca/P 比率为 1.1 的支架在体外显示出更好的生物活性，可诱导钙矿化，促进骨再生。细胞测试表明，所开发的支架可支持前成骨细胞 MC3T3-E1 向成熟成骨细胞的成骨分化和增殖。结果表明，所开发的三维支架在骨组织工程中具有潜在的应用价值。

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来源期刊

Polymers POLYMER SCIENCE-

CiteScore

8.00

自引率

16.00%

发文量

4697

审稿时长

1.3 months

期刊介绍： Polymers (ISSN 2073-4360) is an international, open access journal of polymer science. It publishes research papers, short communications and review papers. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Polymers provides an interdisciplinary forum for publishing papers which advance the fields of (i) polymerization methods, (ii) theory, simulation, and modeling, (iii) understanding of new physical phenomena, (iv) advances in characterization techniques, and (v) harnessing of self-assembly and biological strategies for producing complex multifunctional structures.