The response of DNA methyltransferase and demethylase genes to abiotic stresses in tomato seedling

IF 6.1 2区生物学 Q1 PLANT SCIENCES Plant Physiology and Biochemistry Pub Date : 2024-11-06 DOI:10.1016/j.plaphy.2024.109276

Xuejuan Pan, Zesheng Liu, Li Feng, Chunlei Wang, Chan Liu, Ailing Li, Kangding Yao, Weibiao Liao

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Abstract

DNA methylation plays an important role in regulating plant growth, development and gene expression. However, less is known about the response of DNA methyltransferase and demethylase genes to various stresses. In this study, the effects of abiotic stresses on DNA methylation gene expression patterns in tomato seedlings were investigated. Results showed that most tomato DNA methyltransferase and demethylase genes contained stress-related elements. The expression of SlDML1 was significantly induced by cadmium (Cd) and sodium chloride (NaCl) stresses. SlDML2 was more sensitive and reached its maximum value under polyethylene (PEG) stress at 24 h. The expression of SlMET3L was repressed to varying degrees under Cd, NaCl and PEG stresses at 48 h. However, 5-aza-2′-deoxycytidine (5-azadC) treatment decreased the Cd and PEG stress tolerance by down-regulating the expression of DNA methyltransferase except for the SlMET3L, and up-regulating the expression levels of SlDML2, SlDML3 and SlDML4, cadmium transporters (SlHMA5, SlCAX3, and SlACC3) and osmoregulators (SlDREB, SlLEA and SlHSP70). Whereas 5-azadC treatment alleviated the salt stress through up-regulating DNA methyltransferase gene expression, and down-regulating the expression level of SlDML1, SlDML3, and SlDML4, SlHKT1, SlNHX1, and SlSOS1. Collectively, 5-azadC impaired Cd and PEG stress tolerance and enhanced salt stress tolerance by regulating the expression of methylation-related and stress-related genes in tomato seedlings. These results may provide useful information for further analysing function and evolution of DNA methylation methyltransferase and demethylase genes in tomato under stress conditions.

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番茄幼苗 DNA 甲基转移酶和去甲基化酶基因对非生物胁迫的响应

DNA 甲基化在调节植物生长、发育和基因表达方面发挥着重要作用。然而，人们对 DNA 甲基转移酶和去甲基化酶基因对各种胁迫的反应知之甚少。本研究调查了非生物胁迫对番茄幼苗 DNA 甲基化基因表达模式的影响。结果表明，大多数番茄DNA甲基转移酶和去甲基化酶基因都含有胁迫相关元素。镉（Cd）和氯化钠（NaCl）胁迫显著诱导 SlDML1 的表达。在镉胁迫、氯化钠胁迫和 PEG 胁迫下，SlMET3L 的表达在 48 小时内受到不同程度的抑制。然而，5-氮杂-2'-脱氧胞苷（5-azadC）处理通过下调除SlMET3L以外的DNA甲基转移酶的表达，上调SlDML2、SlDML3和SlDML4、镉转运体（SlHMA5、SlCAX3和SlACC3）和渗透调节剂（SlDREB、SlLEA和SlHSP70）的表达水平，降低了镉和PEG胁迫的耐受性。而5-azadC通过上调DNA甲基转移酶基因的表达，下调SlDML1、SlDML3和SlDML4、SlHKT1、SlNHX1和SlSOS1的表达水平，缓解了盐胁迫。总之，5-azadC 通过调节甲基化相关基因和胁迫相关基因的表达，削弱了番茄幼苗对 Cd 和 PEG 胁迫的耐受性，增强了对盐胁迫的耐受性。这些结果可为进一步分析胁迫条件下番茄 DNA 甲基化甲基转移酶和去甲基化酶基因的功能和进化提供有用信息。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.