Efficacy of different disinfecting methods for contact lenses against Acanthamoeba castellanii.

IF 4.1 3区 医学 Q1 OPHTHALMOLOGY Contact Lens & Anterior Eye Pub Date : 2024-11-09 DOI:10.1016/j.clae.2024.102326
Cristina Pastrana, Fernando Huete-Toral, Ana Privado-Aroco, Gonzalo Carracedo
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Abstract

Purpose: To analyze the efficacy of different disinfecting methods for contact lenses (CL) against Acanthamoeba castellanii (AC) using quantitative PCR (qPCR) based on RNA detection.

Methods: Three CL materials: rigid gas permeable (RGP), hydrogel (Hy), and silicone hydrogel (SiHy), were contaminated with 1x105 amoebae/ml and incubated for 24 h at 30 °C. After contamination, pre-cleaning steps were performed before using four maintenance solutions based on hydrogen peroxide (HP), sodium hypochlorite (SH), povidone-iodine (PI), and a multipurpose solution (MS). The pre-cleaning steps involved using a cleaner (20 % isopropyl alcohol) and rinsing the CL. Disinfection systems 1 and 2 involved no cleaner and rinsed the CL with tap water or saline solution, respectively. Systems 3 and 4 included a cleaner and rinsed with tap water or saline, respectively. After cleaning, A. castellanii was extracted and stored for qPCR analysis, using Hsp70 and TPBF genes to detect RNA A. castellanii. Results were presented as the percentage of positives or negatives (presence or absence of amoebae), with a p-value < 0.05 considered statistically significant.

Results: Disinfection system 1 with MS resulted in 56 % positives for RGP lenses and 100 % positives for both hydrogel materials. When MS was combined with a cleaner, 12.5 % positives were found for SiHy and 100 % negatives for Hy and RGP lenses (p < 0.05). PI solution alone yielded 38 % and 12 % positives for hydrogel and silicone hydrogel lenses, respectively, but was 100 % effective when combined with a cleaner. HP and SH, whether combined with a cleaner or not, were effective against AC for all CL materials, except HP without a cleaner for Hy lenses.

Conclusion: All disinfection methods showed some efficacy against Acanthamoeba on any CL material. The most effective solutions were those based on hydrogen peroxide and sodium hypochlorite. Using a cleaner enhanced the final disinfecting efficacy, especially with the multipurpose solution.

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不同的隐形眼镜消毒方法对卡氏棘阿米巴的功效。
目的:利用基于 RNA 检测的定量 PCR(qPCR)分析不同隐形眼镜(CL)消毒方法对卡氏阿米巴(AC)的功效:用 1x105 阿米巴/毫升污染三种隐形眼镜材料:硬性透气(RGP)、水凝胶(Hy)和硅水凝胶(SiHy),并在 30 °C 下培养 24 小时。污染后,在使用基于过氧化氢(HP)、次氯酸钠(SH)、聚维酮碘(PI)和多用途溶液(MS)的四种维护溶液之前进行预清洁步骤。预清洁步骤包括使用清洁剂(20% 异丙醇)和冲洗 CL。消毒系统 1 和 2 不使用清洁剂,分别用自来水或生理盐水冲洗 CL。系统 3 和 4 含有清洁剂,分别用自来水或生理盐水冲洗 CL。清洗后,提取并储存蓖麻菌,进行 qPCR 分析,使用 Hsp70 和 TPBF 基因检测蓖麻菌 RNA。结果以阳性或阴性(存在或不存在变形虫)的百分比表示,并以 p 值表示 结果:使用 MS 消毒系统 1 后,RGP 镜片的阳性率为 56%,两种水凝胶材料的阳性率均为 100%。当 MS 与清洁剂结合使用时,SiHy 的阳性率为 12.5%,Hy 和 RGP 镜片的阴性率为 100%(p 结论:所有消毒方法都显示出一定的杀菌效果:所有消毒方法对任何 CL 材料上的棘阿米巴都有一定的疗效。最有效的溶液是基于过氧化氢和次氯酸钠的溶液。使用清洁剂可提高最终消毒效果,尤其是多用途溶液。
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来源期刊
CiteScore
7.60
自引率
18.80%
发文量
198
审稿时长
55 days
期刊介绍: Contact Lens & Anterior Eye is a research-based journal covering all aspects of contact lens theory and practice, including original articles on invention and innovations, as well as the regular features of: Case Reports; Literary Reviews; Editorials; Instrumentation and Techniques and Dates of Professional Meetings.
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