Molecular characteristics and antibiotic resistance mechanisms of multidrug-resistant Pseudomonas aeruginosa in Nanning, China.

IF 4 2区生物学 Q2 MICROBIOLOGY BMC Microbiology Pub Date : 2024-11-15 DOI:10.1186/s12866-024-03640-7

Xianzhen Wei, Mingbo Liu, Cuiju Mo, Runxian Tan, Shan Li, Hongjie Liang, Meng Li

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Abstract

Purpose: This study analyzed antibiotic resistance mechanisms and molecular epidemiology of multidrug-resistant Pseudomonas aeruginosa (MDR-PA), aiming at providing clues for prevention and control of MDR-PA infections.

Methods: The carbapenemase resistance genes (VIM, IMP, NDM, KPC, GES, OXA-40) of MDR-PA strains were detected by polymerase chain reaction (PCR) and sequencing. The efflux pump system (MexA, MexC, MexE, MexX), AmpC and OprD2 were detected by real-time fluorescent quantitative PCR (qPCR) in MDR-PA group and sensitive-Pseudomonas aeruginosa (S-PA) group. The homology analysis of MDR-PA strains was performed by multilocus sequence typing (MLST).

Results: A total of 81 MDR-PA strains were collected from the First Affiliated Hospital of Guangxi Medical University from October 2022 to October 2023. Among the carbapenemase detected, the detection rate of NDM-1 was the highest, with a rate of 34.57% (28/81). MexA had a higher expression in MDR-PA group than that in S-PA group (P<0.0001). 81 MDR-PA strains belonged to 40 different ST types, mainly including ST1971, ST244, ST357 and ST308, and the predominant ST type was ST1971 (34.57%, 28/81).

Conclusion: The mechanisms of antibiotic resistance of MDR-PA strains mainly were the production of MBLS and higher expression of MexA in our study, and ST1971 was the predominant ST type of MDR-PA strains in our hospital, our findings may assist in prevention and control of MDR-PA infections.

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中国南宁耐多药铜绿假单胞菌的分子特征和抗生素耐药机制。

目的：本研究分析了耐多药铜绿假单胞菌（MDR-PA）的抗生素耐药机制和分子流行病学，旨在为预防和控制MDR-PA感染提供线索：方法：通过聚合酶链反应（PCR）和测序检测 MDR-PA 菌株的碳青霉烯酶耐药基因（VIM、IMP、NDM、KPC、GES、OXA-40）。通过实时荧光定量 PCR（qPCR）检测了 MDR-PA 组和敏感铜绿假单胞菌（S-PA）组的外排泵系统（MexA、MexC、MexE、MexX）、AmpC 和 OprD2。通过多焦点序列分型（MLST）对MDR-PA菌株进行同源性分析：结果：2022年10月至2023年10月期间，在广西医科大学第一附属医院共采集到81株MDR-PA菌株。在检出的碳青霉烯酶中，NDM-1的检出率最高，为34.57%（28/81）。MexA在MDR-PA组中的表达量高于S-PA组（结论：MDR-PA组中的MexA表达量高于S-PA组）：在我们的研究中，MDR-PA 菌株的抗生素耐药机制主要是 MBLS 的产生和 MexA 的高表达，ST1971 是我院 MDR-PA 菌株的主要 ST 类型，我们的研究结果可能有助于预防和控制 MDR-PA 感染。

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来源期刊

BMC Microbiology 生物-微生物学

CiteScore

7.20

自引率

0.00%

发文量

280

审稿时长

3 months

期刊介绍： BMC Microbiology is an open access, peer-reviewed journal that considers articles on analytical and functional studies of prokaryotic and eukaryotic microorganisms, viruses and small parasites, as well as host and therapeutic responses to them and their interaction with the environment.