A biocompatible fluorescent probe for endogenous hydrogen sulfide detection and imaging

IF 2.6 4区生物学 Q2 BIOCHEMICAL RESEARCH METHODS Analytical biochemistry Pub Date : 2024-11-15 DOI:10.1016/j.ab.2024.115718

Xitian Zhu , Huijia Chen , Fang Ke

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Abstract

Hydrogen sulfide (H₂S) acts as a messenger molecule and can mediate a variety of physiological functions. Conventional methods are seldom used to detect endogenous H₂S and present some difficulties in selective and accurate detection. Reaction-based recognition of endogenous H₂S by organic small molecule probes with good specificity and biocompatibility. To address this challenge, we developed a novel H₂S fluorescent probe 4-(2-(6-hydroxy-2-naphthyl) ethyl)-1-methylpyridinium (DSNP) that triggers a thiolysis reaction through a strong electron withdrawing group, releasing a fluorescent molecule. The simple probe DSNP not only have good selectivity, large Stokes shifts and biocompatibility, but also demonstrated a detection limit as low as 28.4 nM and reaction times as quick as 30 min. Moreover, it has been successfully applied to imaging intracellular H₂S in myeloma cells and zebrafish. This study opens new insights to help push this probe forward for its applicability for detailed H₂S localization studies in osteosarcoma.

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用于内源性硫化氢检测和成像的生物相容性荧光探针。

硫化氢（H2S）是一种信使分子，可介导多种生理功能。传统方法很少用于检测内源性 H2S，而且在选择性和准确检测方面存在一些困难。基于反应的有机小分子探针识别内源性 H2S 具有良好的特异性和生物相容性。为了应对这一挑战，我们开发了一种新型 H2S 荧光探针 4-(2-(6-羟基-2-萘基)乙基)-1-甲基吡啶鎓（DSNP），它能通过一个强取电子基团引发硫解反应，释放出一个荧光分子。简单的探针 DSNP 不仅具有良好的选择性、较大的斯托克斯位移和生物相容性，而且检测限低至 28.4 nM，反应时间短至 30 分钟。此外，它还被成功应用于骨髓瘤细胞和斑马鱼细胞内 H2S 的成像。这项研究开启了新的视角，有助于推动该探针在骨肉瘤 H2S 定位详细研究中的应用。

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来源期刊

Analytical biochemistry 生物-分析化学

CiteScore

5.70

自引率

0.00%

发文量

283

审稿时长

44 days

期刊介绍： The journal''s title Analytical Biochemistry: Methods in the Biological Sciences declares its broad scope: methods for the basic biological sciences that include biochemistry, molecular genetics, cell biology, proteomics, immunology, bioinformatics and wherever the frontiers of research take the field. The emphasis is on methods from the strictly analytical to the more preparative that would include novel approaches to protein purification as well as improvements in cell and organ culture. The actual techniques are equally inclusive ranging from aptamers to zymology. The journal has been particularly active in: -Analytical techniques for biological molecules- Aptamer selection and utilization- Biosensors- Chromatography- Cloning, sequencing and mutagenesis- Electrochemical methods- Electrophoresis- Enzyme characterization methods- Immunological approaches- Mass spectrometry of proteins and nucleic acids- Metabolomics- Nano level techniques- Optical spectroscopy in all its forms. The journal is reluctant to include most drug and strictly clinical studies as there are more suitable publication platforms for these types of papers.