Blood serum interleukins levels and their relationship with colon tissue prostanoids in experimental ulcerative colitis.

Abstract

Objective: Aim: To study IL-1β, IL-6, IL-8, IL-10 blood serum levels and their relationship with colon tissue prostanoids in experimental ulcerative colitis.

Patients and methods: Materials and Methods: Blood serum interleukins and colon tissue prostanoids were determined by enzyme immunosorbent assay on three groups of WAG population rats (1st control group - rectal saline injection; 2nd control group - 50% ethanol injection; experimental group - injection of 2,4-dinitrobenzene-sulfonic acid in 50% ethanol).

Results: Results: The increased levels of all interleukins both in 2nd control and experimental groups versus 1st control group were found. No dif f erences in IL-6, IL-8 levels between experimental and 2nd control groups were revealed. The relationships between interleukins and PGF2α, between interleukins and TXB2 in all groups, and the appearance of correlations between interleukins and PGE2, between interleukins and PGI2 in experimental group were observed. Negative correlations between IL-8 and COX-2 in 1st control, between IL-8 and COX-1 in both 2nd control and experimental groups were found. Negative correlation between 8-epi-PGF2α and IL-8, positive correlation between 8-epi-PGF2α and PGF2α in experimental group were revealed.

Conclusion: Conclusions: Results obtained testify the possible role of PGF2α and 8-epi-PGF2α in the development of ulcerative colitis and justify the importance of research on the link of 8-epi-PGF2α, IL-8, PGF2α for understanding the mechanisms of development and progression of this disease. Increasing blood serum IL-8 level may be used as marker of reducing colon tissue COX-1 content in both ethanol- and 2,4-dinitrobenzenesulfonic acid-induced ulcerative colites.