Combining citrus waste-derived function microbes with biochar promotes humus formation by enhancing lignocellulose degradation in citrus waste compost

Abstract

The low degradation rate of lignocellulose limits the humification process of citrus organic waste composting. This study explored the roles of general microbial inoculation (GM), citrus waste-derived function microbial inoculation (CM), and CM combined with biochar (CMB) in citrus waste compost. Results showed microbial inoculations all promoted lignocellulose degradation and humus formation, but the roles of CM and CMB were better than GM, especially CMB. Compared to the control, CMB raised the temperature and duration of thermophilic phase by 2.8 °C and 4 days, and improved lignin degradation rate and humus content by 21.5% and 7.6%. Furthermore, CMB promoted bacterial community succession and cooperation, and decreased network complexity. Moreover, CMB strengthened the correlation between mainly bacterial communities and polysaccharides, reducing sugars as well as carbohydrates metabolic, enhancing the contribution of bacteria such as Bacillus, Flavobacterium and Staphylococcus to humus and its precursors. It concludes that the naturally derived microbes in compost had better effects on promoting humus synthesis than exogenous microbes, which provides a new route for rapid humification of high-lignin organic waste in composting.