Removal of terpenes in the presence of easily degradable compounds during biofiltration of gas emissions from composting of municipal solid waste

Abstract

Composting of the organic fraction of municipal solid waste (OFMSW) is accompanied by the emission of large volumes of harmful, hazardous and foul-smelling volatile organic compounds (VOCs). To improve the efficiency of terpenes removal, which constitute a significant part of VOCs, pure cultures of microorganisms dominating in its microbiota were isolated from the microbial community of the biofilter, which has been cleaning such emissions for a long time. Seven pure cultures were isolated and then tested for being able to grow on a mineral medium in the presence of terpene vapor as the only source of carbon and energy. Three of the most actively growing cultures were selected, characterized and identified by the 16S rRNA gene as Rhodococcus erythropolis CA1, Rhodococcus pyridinivorans CA3 and Gordonia sp. CA6. Three identical laboratory biofilters (BF) were inoculated with a mix of these cultures to test the possibility of more complete removal of terpenes. Biofilters were adapting to clearing the model mix of terpineols and geraniol vapors for 45 days. During 45 days the purification efficiency of the model mix reached an average of 91.5% with a contact time (CT) of 3.7 ± 0.2 s and the terpene vapors concentration of 14 ± 2 mg m⁻³. Then the biofilters number BF2.1 and BF3.1 were connected to real emission from composting OFMSW. The biofilter BF2.1 purified the emission directly, whereas BF3.1 purified similar discharge after the intermediate biofilter of the 1st stage of purification (BF0.0). The BF1.0 was left connected to purification of the model mix as a control. The effectiveness of biofiltration of hard-to-remove terpenes was evaluated by gas chromatography of samples taken at the inlet and outlet of biofilters. The average efficiency of removing terpenes from real emissions by BF2.1 was 93.1 % (CT = 5.5 s). The total efficiency of removing terpenes by (BF0.0 + BF3.1) complex was 93.2 % (total CT = 7.4 s). A study of the microbiota of inoculated biofilters after 60 and 90 days of purification the real emission by cultivation from dilutions, identification by the 16S rRNA gene and fingerprinting showed that in BF2.1 and BF3.1 Rhodococcus erythropolis CA1 and Rhodococcus pyridinivorans CA3 were preserved among living cells at a level of 6.5–12.4 %, and genetically fully corresponded to the original cultures. These results could have a positive impact on improving the results of deodorization of emissions from OFMSW composting by biofiltration, simplifying the design of the biofiltration facility (one stage instead of two) and reducing the total time for effective biofiltration. This, in turn, would contribute to the wider introduction of highly efficient emission purification methods at OFMSW composting facilities in order to create more comfortable and ecologically clean environmental conditions around them.