A facile optical sensing strategy for glyphosate detection based on structure-switching signaling aptamers

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL Microchimica Acta Pub Date : 2024-11-18 DOI:10.1007/s00604-024-06839-7

Ziping Liu, Xin Wang, Edith Bai, Yuhan Zhao, Shasha Liu, Zhiwei Xu, Qing Chang, Xinru Huang, Ye Tian

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Abstract

A facile and highly specific optical sensing strategy is established for glyphosate (GLYP) detection using structure-switching signaling aptamers (F-SSSAs) with fluorescence signal reporting functionality. The strategy involves two domains: the FITC-labeled signal transduction domain for fluorescence signal reporting, while the functional domain (specific structure-switching aptamers) controls the target recognition. Graphene oxide (GO) works as a robust F-SSSAs quencher in the absence of GLYP. However, the F-SSSAs structure is switched in the presence of GLYP, prominently affecting the interaction with GO. The fluorescence of the structure-switching signaling aptamer-based sensing system is subsequently restored. The present strategy exhibits two dynamic linear relationships for GLYP detection in the ranges 0.2 to 80 ng·mL⁻¹ and 100 to 800 ng·mL⁻¹, with a low detection limit (LOD) of 0.07 ng·mL⁻¹. Significantly, the proposed sensing system has been successfully utilized to detect GLYP in water, soil, and rice, demonstrating its potential applications in GLYP monitoring.

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基于结构转换信号适配体的草甘膦简便光学传感检测策略。

利用具有荧光信号报告功能的结构转换信号配体（F-SSSA），建立了一种简便且高度特异的草甘膦（GLYP）光学传感检测策略。该策略涉及两个结构域：FITC 标记的信号转导结构域用于荧光信号报告，而功能结构域（特异性结构转换适配体）则控制目标识别。在没有 GLYP 的情况下，氧化石墨烯（GO）可作为一种稳定的 F-SSSAs 淬灭剂。然而，在有 GLYP 的情况下，F-SSA 的结构会发生转换，从而显著影响与 GO 的相互作用。基于结构切换信号肽的传感系统的荧光随后恢复。在 0.2 至 80 毫微克/毫升-1 和 100 至 800 毫微克/毫升-1 的范围内，本策略对 GLYP 的检测呈现出两种动态线性关系，低检测限（LOD）为 0.07 毫微克/毫升-1。值得注意的是，所提出的传感系统已成功用于检测水、土壤和水稻中的 GLYP，证明了其在 GLYP 监测中的潜在应用。

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.