Genetic mapping and quantitative trait loci analysis for pistillate flowers per node and multi-pistillate flower traits in the F₂ cucumber population.

IF 2 4区农林科学 Q2 AGRONOMY Breeding Science Pub Date : 2024-06-01 Epub Date: 2024-06-18 DOI:10.1270/jsbbs.23070

Nattawat Anankul, Wannapa Sattayachiti, Namfon Onmanee, Saengchit Chanmoe, Weenun Bundithya, Jutamas Kumchai

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Abstract

This study focused on cucumbers' multi-pistillate flower (MPF) trait, which is essential for high yields. A genetic linkage map was constructed using a population of 219 F₂ plants to analyze quantitative trait loci (QTL) associated with MPF traits. Crossbreeding of EWSCU-809 (MPF) with EWSCU-989 (single pistillate flower: SPF) generated an F₁ hybrid that self-pollinated to form an F₂ population. Based on 244 single nucleotide polymorphic markers across seven cucumber chromosomes, a linkage map facilitated QTL analysis considering average pistillate flowers (PFs) per node and nodes with MPF traits. The results indicated a 9:6:1 epistatic ratio in the F₂ populations, revealing recessive allele control of the MPF trait in gynoecious plants. Three QTLs (qMP2.1, qMP3, qMP7) on chromosomes 2, 3, and 7 were associated with average PFs per node, explaining 5.6 to 10.3% of phenotypic variation. Four QTLs (qMP2.2, qMP3, qMP4, qMP7) on chromosomes 2, 3, 4, and 7 were linked to the presence of nodes with MPF traits, explaining 5.8 to 10.6% of phenotypic variance. Notably, QTL regions overlapped between the two datasets, suggesting pleiotropic effects, particularly on chromosomes 3 and 7. These reliable QTLs have the potential to improve breeding programs, enhance PF development, and increase cucumber yields.

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黄瓜 F2 群体中每节雌花和多雌花性状的遗传图谱和数量性状位点分析。

本研究的重点是黄瓜的多雌花（MPF）性状，该性状对黄瓜的高产至关重要。利用 219 株 F2 群体构建了遗传连锁图谱，以分析与 MPF 性状相关的数量性状位点（QTL）。EWSCU-809（MPF）与EWSCU-989（单雌花：SPF）杂交产生了一个F1杂种，该杂种通过自花授粉形成了一个F2群体。基于 7 条黄瓜染色体上的 244 个单核苷酸多态性标记，考虑到每个节点的平均雌花（PFs）和具有 MPF 性状的节点，连锁图谱促进了 QTL 分析。结果表明，F2 群体中的外显率为 9:6:1，揭示了雌雄同株中 MPF 性状的隐性等位基因控制。染色体 2、3 和 7 上的三个 QTL（qMP2.1、qMP3 和 qMP7）与每节平均 PFs 有关，解释了 5.6% 至 10.3% 的表型变异。染色体 2、3、4 和 7 上的四个 QTL（qMP2.2、qMP3、qMP4、qMP7）与具有 MPF 性状的节点的存在有关，解释了 5.8% 至 10.6% 的表型变异。值得注意的是，两个数据集之间的 QTL 区域重叠，表明存在多效应，尤其是在 3 号和 7 号染色体上。这些可靠的 QTLs 有潜力改进育种计划、促进 PF 的发展并提高黄瓜产量。

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来源期刊

Breeding Science 农林科学-农艺学

CiteScore

4.90

自引率

4.20%

发文量

审稿时长

1.5 months

期刊介绍： Breeding Science is published by the Japanese Society of Breeding. Breeding Science publishes research papers, notes and reviews related to breeding. Research Papers are standard original articles. Notes report new cultivars, breeding lines, germplasms, genetic stocks, mapping populations, database, software, and techniques significant and useful for breeding. Reviews summarize recent and historical events related breeding. Manuscripts should be submitted by corresponding author. Corresponding author must have obtained permission from all authors prior to submission. Correspondence, proofs, and charges of excess page and color figures should be handled by the corresponding author.