Therapeutic potential of Tithonia diversifolia extract: Modulating IL-35, TNF-α, and hematology profile in streptozotocin-induced rat model.

IF 1 Q3 VETERINARY SCIENCES Open Veterinary Journal Pub Date : 2024-09-01 Epub Date: 2024-09-30 DOI:10.5455/OVJ.2024.v14.i9.12

Rondius Solfaine, Iwan Sahrial Hamid, Kurnia Desiandura

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Abstract

Background: Diabetes mellitus is a significant global health issue with increasing prevalence worldwide.

Aim: This study aims to investigate the potential of Tithonia diversifolia extract (TE) in lowering interleukin-35 (IL-35), tumor necrosis factor-alpha (TNF-α), and hematological profile in streptozotocin (STZ)-induced rats.

Methods: A total of 24 rats were divided into four treatment groups: control (P0), diabetic induction (P1), diabetic induction + TE (P2), and diabetic induction + quercetin (P3). Diabetes mellitus was induced by a single-dose injection of stz (60 mg/kg). TE treatment was administered orally for 7 days. On the 8th day post-treatment, all animals were euthanized, and blood samples were collected to assess inflammatory parameters, including IL-35, TNF-α, GPx, and hematological profiles. Kidney organs were fixed in 10% buffered neutral formalin for histopathological analysis. Data were analyzed using ANOVA followed by Duncan's test (p < 0.05).

Results: Evaluation of the hematological profile revealed significant improvements in the P2 and P3 groups, with decreased leukocytes, hemoglobin, lymphocytes, and neutrophils, as well as significantly lower IL-35 and TNF-α levels observed in diabetic rats following TE treatment.

Conclusion: TE treatment exhibited promising effects in reducing inflammatory markers and restoring hematological parameters in diabetic rats, indicating its potential as a therapeutic agent in diabetic rats.

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Tithonia diversifolia 提取物的治疗潜力：在链脲佐菌素诱导的大鼠模型中调节 IL-35、TNF-α 和血液学特征。

背景：目的：本研究旨在探讨多样性蒺藜提取物（TE）在降低链脲佐菌素（STZ）诱导大鼠白细胞介素-35（IL-35）、肿瘤坏死因子-α（TNF-α）和血液学特征方面的潜力：将 24 只大鼠分为四个治疗组：对照组（P0）、糖尿病诱导组（P1）、糖尿病诱导 + TE 组（P2）和糖尿病诱导 + 槲皮素组（P3）。通过单剂量注射 stz（60 毫克/千克）诱发糖尿病。口服 TE 治疗 7 天。治疗后第 8 天，对所有动物实施安乐死，并采集血液样本以评估炎症参数，包括 IL-35、TNF-α、GPx 和血液学特征。将肾脏器官固定在 10% 的缓冲中性福尔马林中进行组织病理学分析。采用方差分析和邓肯检验对数据进行分析（P < 0.05）：结果：对血液学特征的评估显示，P2 组和 P3 组糖尿病大鼠的血液学特征明显改善，白细胞、血红蛋白、淋巴细胞和中性粒细胞减少，IL-35 和 TNF-α 水平明显降低：结论：TE 治疗在降低糖尿病大鼠的炎症指标和恢复血液学参数方面表现出良好的效果，表明其具有作为糖尿病大鼠治疗药物的潜力。

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来源期刊

Open Veterinary Journal VETERINARY SCIENCES-

CiteScore

1.40

自引率

0.00%

发文量

112

审稿时长

12 weeks

期刊介绍： Open Veterinary Journal is a peer-reviewed international open access online and printed journal that publishes high-quality original research articles. reviews, short communications and case reports dedicated to all aspects of veterinary sciences and its related subjects. Research areas include the following: Infectious diseases of zoonotic/food-borne importance, applied biochemistry, parasitology, endocrinology, microbiology, immunology, pathology, pharmacology, physiology, epidemiology, molecular biology, immunogenetics, surgery, ophthalmology, dermatology, oncology and animal reproduction. All papers are peer-reviewed. Moreover, with the presence of well-qualified group of international referees, the process of publication will be done meticulously and to the highest standards.