The gut microbiome and resistome of yellow perch (Perca flavescens) living in Minnesota lakes under varying anthropogenic pressure

IF 4.1 2区 医学 Q1 INFECTIOUS DISEASES One Health Pub Date : 2024-11-12 DOI:10.1016/j.onehlt.2024.100933
Omar Jimenez-Lopez , Tui Ray , Christopher Dean , Ilya Slizovskiy , Jessica Deere , Tiffany Wolf , Seth Moore , Alexander Primus , Jennifer Høy-Petersen , Silje Finstad , Jakob Mo , Henning Sørum , Noelle Noyes
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Abstract

Anthropogenic activities can significantly impact wildlife in natural water bodies, affecting not only the host's physiology but also its microbiome. This study aimed to analyze the gut microbiome and antimicrobial resistance gene profile (i.e., the resistome) of yellow perch living in lakes subjected to different levels of anthropogenic pressure: wastewater effluent-impacted lakes and undeveloped lakes. Total DNA and RNA from gut content samples were extracted and sequenced for analysis. Results indicate that the gut resistome and microbiome of yellow perch differ between lakes, perhaps due to varying anthropogenic pressure. The resistome was predominated by macrolide resistance genes, particularly the MLS23S group, making up 53 % of resistome sequences from effluent-impacted lakes and 73 % from undeveloped lakes. The colistin resistance gene group (mcr) was detected in numerous samples, including variants associated with Aeromonas and the family Enterobacteriaceae. The gut microbiome across all samples was dominated by the phyla Proteobacteria, Firmicutes, and Actinobacteria, with the opportunistic pathogens Plesiomonas shigelloides and Aeromonas veronii more abundant in effluent-impacted lakes. Metagenomic analysis of wild fish samples offers valuable insights into the effects of anthropogenic pressures on microbial communities, including antimicrobial resistance genes, in water bodies.
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生活在明尼苏达州湖泊中的黄鲈(Perca flavescens)在不同人为压力下的肠道微生物组和抗性组
人为活动会对自然水体中的野生动物产生重大影响,不仅会影响宿主的生理机能,还会影响其微生物组。本研究旨在分析生活在受到不同程度人为压力的湖泊(受废水影响的湖泊和未开发的湖泊)中的黄鲈的肠道微生物组和抗菌素耐药性基因谱(即耐药性组)。从肠道内容物样本中提取总 DNA 和 RNA 并进行测序分析。结果表明,不同湖泊的黄鲈肠道抗性组和微生物组存在差异,这可能是由于人为压力不同造成的。耐药性基因组以大环内酯类耐药性基因为主,尤其是 MLS23S 基因组,占受污水影响湖泊耐药性基因组序列的 53%,占未开发湖泊耐药性基因组序列的 73%。许多样本中都检测到了可乐定耐药基因组(mcr),包括与气单胞菌和肠杆菌科相关的变种。所有样本的肠道微生物组均以变形菌门、固形菌门和放线菌门为主,机会致病菌 Plesiomonas shigelloides 和 Aeromonas veronii 在受污水影响的湖泊中含量更高。对野生鱼类样本的元基因组分析为了解人类活动对水体微生物群落(包括抗菌药耐药性基因)的影响提供了宝贵的信息。
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来源期刊
One Health
One Health Medicine-Infectious Diseases
CiteScore
8.10
自引率
4.00%
发文量
95
审稿时长
18 weeks
期刊介绍: One Health - a Gold Open Access journal. The mission of One Health is to provide a platform for rapid communication of high quality scientific knowledge on inter- and intra-species pathogen transmission, bringing together leading experts in virology, bacteriology, parasitology, mycology, vectors and vector-borne diseases, tropical health, veterinary sciences, pathology, immunology, food safety, mathematical modelling, epidemiology, public health research and emergency preparedness. As a Gold Open Access journal, a fee is payable on acceptance of the paper. Please see the Guide for Authors for more information. Submissions to the following categories are welcome: Virology, Bacteriology, Parasitology, Mycology, Vectors and vector-borne diseases, Co-infections and co-morbidities, Disease spatial surveillance, Modelling, Tropical Health, Discovery, Ecosystem Health, Public Health.
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