One Health最新文献

{"title":"Integrated meta-analysis and sentinel surveillance: Global prevalence and risk factors for Mycoplasma bovis in cattle (2007–2023)","authors":"Sen Zhang ,&nbsp;Guoxing Liu ,&nbsp;Fansong Yu ,&nbsp;Jiyu Zhang ,&nbsp;Xuzheng Zhou ,&nbsp;Aizhen Guo ,&nbsp;Yingyu Chen","doi":"10.1016/j.onehlt.2026.101318","DOIUrl":"10.1016/j.onehlt.2026.101318","url":null,"abstract":"<div><h3>Background</h3><div><em>Mycoplasma bovis</em> (<em>M. bovis</em>) is a major pathogen of cattle and contributes to substantial economic losses worldwide. A comprehensive understanding of its global prevalence is lacking, hindering the development of effective control strategies.</div></div><div><h3>Objectives</h3><div>This study aimed to determine the global pooled prevalence of <em>M. bovis</em>, identify key factors influencing its epidemiology, and validate the findings with recent regional surveillance data.</div></div><div><h3>Methods</h3><div>A systematic review and meta-analysis were conducted following the preferred reporting items for systematic reviews and meta-analyses (PRISMA) guidelines. A literature search was performed across four databases in June 2025, encompassing studies published between 2007 and 2023. Additionally, 979 nasal swab samples from Chinese herds from 2021 to 2024 were analyzed using conventional PCR.</div></div><div><h3>Results</h3><div>Twenty-three studies from 13 countries were included. The global pooled prevalence was 24.5 % (95 % CI: 12.7 %–38.7 %), with extremely high heterogeneity (<em>I</em>^<em>2</em> = 99.9 %). Our independent laboratory data also revealed a nearly identical positivity level of 21.9 % in Chinese herds from 2021 to 2024. Subgroup analyses identified age as the most significant risk factor, with a dramatically greater prevalence in young stock (&lt; 12 months) (56.2 %) than in adults (6.7 %) (<em>p</em> = 0.01). A relatively high prevalence was also associated with beef production systems, male cattle, serum samples, and culture method. Sensitivity analysis and publication bias assessment confirmed the robustness of the findings.</div></div><div><h3>Conclusion</h3><div>This study provides the first robust estimate of the global prevalence of <em>M. bovis</em>, validated by contemporary surveillance data. These findings underscore the severe burden of infection in young stock and provide crucial evidence to inform targeted surveillance and control programs in the global cattle industry.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"22 ","pages":"Article 101318"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145927018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of water quality and associated health risks in community-managed water systems in highland areas: A case study Chiang Rai province, Thailand","authors":"Suntorn Sudsandee ,&nbsp;Wissanupong Kliengchuay ,&nbsp;Vivat Keawduanglek ,&nbsp;Anuttara Hongtong ,&nbsp;Weerayuth Siriratruengsuk ,&nbsp;Sarima Niampradit ,&nbsp;Rachaneekorn Mingkhwan ,&nbsp;Nuttapohn Kiangkoo ,&nbsp;Nopadol Precha ,&nbsp;Kraichat Tantrakarnapa","doi":"10.1016/j.onehlt.2025.101290","DOIUrl":"10.1016/j.onehlt.2025.101290","url":null,"abstract":"<div><h3>Background</h3><div>The present study addresses the persistent challenges associated with access to safe drinking water in highland communities of Thailand. Informal water supply systems, often managed by local villagers, rely heavily on untreated groundwater and surface water sources. This study assessed the quality of community-managed water supply systems and the associated health risks in Bandu and Nang Lae sub-districts, Chiang Rai Province, Thailand.</div></div><div><h3>Methods</h3><div>To evaluate the quality of these community-managed water systems, a total of 66 water samples from 22 villages were collected and analyzed for physical, chemical, and biological parameters. Heavy metal concentrations were measured, and health risk assessments were conducted through dermal and oral exposure routes based on U.S. EPA guidelines. The One health concept was integrated to explain the association among human, animal, and ecological systems.</div></div><div><h3>Results</h3><div>Monitoring data showed that many parameters exceeded the Provincial Waterworks Authority (PWA)’s standard, including pH (41 %), iron (32 %), lead (5 %), turbidity (2 %), mercury (2 %), and lack of free residual chlorine (100 %). The water samples showed signs of biological contamination. Total coliform bacteria were found in 39 % of the samples, fecal coliform bacteria in 27 %, and <em>Escherichia coli</em> in 9 %. Health risk assessments indicated that hazard quotients (HQs) and hazard indices (HIs) for heavy metals were less than 1, suggesting a negligible health risk. Total coliform bacteria levels in household water samples were positively correlated with diarrhea incidence (<em>r</em> = 0.54, <em>p</em> &lt; 0.05).</div></div><div><h3>Conclusion</h3><div>However, a positive correlation was observed between total coliform presence and reported diarrhea cases. The findings underscore the urgent need for improved water treatment practices, animal excreta management, ecological systems, and government interventions to ensure access to a safe water supply for highland communities in Thailand, which are under the one health concept. However, it highlights the need to focus on water quality, as in SDG 6 ensuring the availability and sustainable management of water and sanitation for all.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"22 ","pages":"Article 101290"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145712267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The promise of one health for improved soil and food security in Papua New Guinea","authors":"Tom Swan ,&nbsp;Josephine Saul Maora ,&nbsp;Barbara Pamphilon ,&nbsp;Damien Field","doi":"10.1016/j.onehlt.2026.101341","DOIUrl":"10.1016/j.onehlt.2026.101341","url":null,"abstract":"<div><div>Papua New Guinea (PNG) faces a critical food security crisis, with nearly half of children under five experiencing stunting—more than twice the global average. Combined with high rates of wasting and overweight, this reflects the country's ‘double burden of malnutrition’. Furthermore, public health and environmental pressures in PNG are intensifying, particularly as climate change reduces crop yields, nutrient density and ecosystem stability. Addressing these interconnected challenges demands integrated strategies that strengthen both food and soil security. This article applies the One Health framework to PNG's social-ecological system by examining two interventions: soil health restoration and mangrove ecosystem rehabilitation. These interventions enhance landscape resilience, restore degraded soils and sustain protein-rich food sources. Our novel PNG One Health (<em>Wanpela Helt</em>) framework—a culturally grounded approach—emphasises strong soil, strong food and strong community (<em>strongpela giraun, strongpela kaikai, strongpela komuniti</em>), with family, community-based approaches and gender equity as cornerstones for sustainable development.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"22 ","pages":"Article 101341"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146142986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Key aspects of One Health governance in the European Union","authors":"Fergal Donnelly ,&nbsp;Nikolaos I. Stilianakis","doi":"10.1016/j.onehlt.2026.101325","DOIUrl":"10.1016/j.onehlt.2026.101325","url":null,"abstract":"<div><div>One Health governance involves the integrated coordination and management of policies, programs, and activities addressing the interconnected health of humans, animals, and the environment. Its development and implementation necessitate collaboration and coordination across various sectors and require significant resources. Consequently, key performance indicators are essential for monitoring progress and informing adaptations. Additionally, integrative surveillance systems encompassing human, animal, and environmental health sources, along with digital technologies and interoperable data systems, are crucial components for effective governance. These elements collectively foster health and well-being in a holistic manner.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"22 ","pages":"Article 101325"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145977198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Climate-driven invasion of Pomacea canaliculata and Angiostrongylus cantonensis transmission risk: Ecological niche modeling forecasts and public-health governance recommendations","authors":"Hongyu Li ,&nbsp;Yuqing Wang ,&nbsp;Yihan Chen ,&nbsp;Chaojun Shan ,&nbsp;Jiyuan Wang ,&nbsp;Ruke Wang ,&nbsp;Yuqing Shao ,&nbsp;Jialu Xu ,&nbsp;Xiaodong Yao ,&nbsp;Keda Chen","doi":"10.1016/j.onehlt.2025.101315","DOIUrl":"10.1016/j.onehlt.2025.101315","url":null,"abstract":"<div><div>Global climate change is rapidly reshaping species distribution patterns worldwide. <em>Pomacea canaliculata</em> (golden apple snail), an important invasive species in many countries, has experienced accelerated range expansion driven by climate change and anthropogenic activities. Owing to its high phenotypic plasticity, broad environmental tolerance, and high fecundity, this species causes severe agricultural damage and biodiversity loss. Importantly, <em>P. canaliculata</em> is a highly competent intermediate host of the rat lungworm <em>Angiostrongylus cantonensis</em>, the causative agent of human eosinophilic meningitis, posing a growing public health threat. This article synthesizes the biological traits, invasion mechanisms, and ecological impacts of <em>P. canaliculata</em>, together with the transmission cycle and epidemiology of <em>A. cantonensis</em>. Using the Maximum Entropy (MaxEnt) model under two future climate scenarios (SSP2–4.5 and SSP5–8.5), we projected the spatiotemporal dynamics of suitable habitat for <em>P. canaliculata</em>. Current distributions are primarily driven by hydrothermal factors, with annual precipitation (BIO12) and maximum temperature of the warmest month (BIO5) exerting dominant influences, resulting in high suitability across tropical and subtropical regions. Under future warming, habitat suitability is projected to decline in low-latitude regions while expanding poleward, with increased fragmentation under SSP5–8.5. High-stability resilience hotspots, including the lower Yangtze River Basin and the Mississippi River Basin, are identified as priority areas for management. Based on these projections, we propose a precision governance framework integrating niche-based risk stratification, low-toxicity biocontrol, habitat management, cross-border surveillance, and public education to support climate-adaptive prevention of invasive species and parasite transmission.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"22 ","pages":"Article 101315"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145977196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Turning urban wildlife mortality into a surveillance tool: Detection of vector-borne pathogens in carcasses of hedgehogs, squirrels, and blackbirds","authors":"Karolina Volfová ,&nbsp;Václav Hönig ,&nbsp;Michal Houda ,&nbsp;Petr Papežík ,&nbsp;Paulina Maria Lesiczka ,&nbsp;Manoj Fonville ,&nbsp;Hein Sprong ,&nbsp;Barbora Černá Bolfíková ,&nbsp;Pavel Hulva ,&nbsp;Daniel Růžek ,&nbsp;Lada Hofmannová ,&nbsp;Jan Votýpka ,&nbsp;David Modrý","doi":"10.1016/j.onehlt.2026.101328","DOIUrl":"10.1016/j.onehlt.2026.101328","url":null,"abstract":"<div><div>Tick-borne zoonoses pose a major challenge to human and animal health, driving efforts to monitor the distribution, intensity, and diversity of their causative agents. Within the One Health framework, which links human, animal, and environmental health, integrated surveillance strategies are increasingly needed. However, most studies focus on tick vectors, while vertebrate reservoirs are often overlooked due to labour-intensive sampling, the need for specialized skills, and legislative or species protection constraints.</div><div>This study evaluated whether carcasses of accidentally killed wildlife (primarily roadkill) can serve as a source of biological material for vector-borne pathogen surveillance, with a focus on urban habitats due to their public health relevance. Hedgehogs, squirrels, and blackbirds were selected as synanthropic species that thrive in cities, are commonly infested by ticks, and act as hosts for zoonotic tick-borne pathogens (TBPs).</div><div>A total of 268 carcasses (125 hedgehogs, 55 squirrels, and 88 blackbirds) were collected across multiple Czech cities with public assistance. Overall, 1836 tissue samples were analyzed using multiplex real-time PCR assays targeting over ten microorganisms. Detection efficiency was compared across tissues, with ear and skin consistently the most reliable and versatile sample types. Individual pathogen-host-tissue combinations reached 65–93% efficiency, highlighting the value of multi-tissue sampling. The most prevalent TBPs detected were <em>Anaplasma phagocytophilum</em>, <em>Borrelia burgdorferi</em> s.l., <em>Bartonella</em> spp., and <em>Rickettsia helvetica</em>.</div><div>In conclusion, carcasses of accidentally killed urban wildlife provide a practical and valuable resource for TBP surveillance, complementing vector-focused methods. This approach supports One Health principles by integrating wildlife monitoring into urban disease surveillance efforts.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"22 ","pages":"Article 101328"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145977199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"One health rapid qualitative assessment: Exploring local governance gaps in Tanzania","authors":"Olivier Rubin ,&nbsp;Suzana S. Nyanda ,&nbsp;Madelaine Norström","doi":"10.1016/j.onehlt.2026.101353","DOIUrl":"10.1016/j.onehlt.2026.101353","url":null,"abstract":"<div><div>The One Health Approach (OHA) is globally recognized as essential for addressing complex health threats such as antimicrobial resistance (AMR). However, translating national commitments into coordinated subnational practice remains a major governance challenge. This paper presents the OH Rapid Qualitative Assessment (OH-RQA) as a rapid, resource-efficient diagnostic tool for analyzing subnational implementation. Using five analytical dimensions (thinking, planning, working, sharing, and learning) the OH-RQA examines how practitioners and implementers at the local level understand and apply the OHA.</div><div>We applied the OH-RQA in two Tanzanian regions in the context of AMR, collecting data through focus group discussions and semi-structured interviews with district officials in human, animal, and environmental sectors. Findings reveal persistent gaps between national OHA strategies and local governance practices, including limited cross-sectoral coordination, inconsistent awareness of national AMR priorities, and structural barriers to integrated action. Officers identified absent policy mandates, insufficient guidance from higher administrative levels, lack of training and incentives, and the exclusion of OHA from most job descriptions as key obstacles.</div><div>By providing a scalable, structured, qualitative diagnostic framework focused on local practice, the OH-RQA supports targeted recommendations for strengthening subnational AMR governance. Beyond Tanzania, it offers a transferable approach for rapidly assessing OH implementation across diverse crisis governance contexts, including pandemic preparedness, climate adaptation, and cross-border health threats.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"22 ","pages":"Article 101353"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146188442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A claim for plant health as a key component of the one health concept","authors":"Ramon Albajes ,&nbsp;María M. López ,&nbsp;Rafael M. Jiménez Díaz","doi":"10.1016/j.onehlt.2025.101304","DOIUrl":"10.1016/j.onehlt.2025.101304","url":null,"abstract":"<div><div>The concept of health has historically been more closely linked to the well-being of humans than to that of animals, plants or the environment. In contrast, the One Health concept, which emerged in recent decades, generally recognizes the interdependence of only three of its four components: humans, wild and domestic animals, and the environment, but plants have often been overlooked in this analysis. Because plant health has been undervalued within the One Health framework, we emphasize in this review its importance in ensuring food security and safety, two key issues in human and animal health, as highlighted in several of the United Nations SDG. Food production, marketing and consumption use a significant portion of the environment's natural resources, and plant health technology must ensure their sustainable use while safeguarding environmental health. We summarize the relationships between the four components of One Health, highlighting the development of antimicrobial resistance in human, animal and plant pathogens, and the resistance to plant protection products among plant pests, diseases and weeds. Three external drivers significantly influence plant health and One Health in the last decades: climate change, invasive alien species, and the international armed conflicts. The main reported effects of climate change on plant health include the shifts in distribution area, biology and life cycles of harmful organisms as well as plant-pest interactions. Another major factor compromising the sustainability of current plant health technology is the introduction and establishment of alien organisms affecting vegetables resulting from the increasing globalization of food trade, human labour and tourism.These challenges underscore the need to approach One Health at broader scales, beyond individual organisms or populations, as well as intensifying studies on plant health, to effectively address holistically the complex, interconnected risks affecting global health.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"22 ","pages":"Article 101304"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145927024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and distribution of Echinococcus species in domestic dogs and wild canids in Pennsylvania: KeyScreen® GI Parasite PCR testing of fecal samples","authors":"Kayla B. Garrett ,&nbsp;Justin Brown ,&nbsp;Pablo D. Jimenez Castro ,&nbsp;Michelle Evason ,&nbsp;Maria Jarque ,&nbsp;Emily Jenkins ,&nbsp;Noelle Kuhn ,&nbsp;Christian Leutenegger ,&nbsp;Cecilia Lozoya ,&nbsp;Samantha Loo ,&nbsp;Lauren Maxwell ,&nbsp;Kevin D. Niedringhaus ,&nbsp;Andrew S. Peregrine ,&nbsp;Christian Savard ,&nbsp;Jeffrey Tereski ,&nbsp;Taylor Volappi ,&nbsp;Kyle Van Why ,&nbsp;Michael J. Yabsley ,&nbsp;Christopher A. Cleveland","doi":"10.1016/j.onehlt.2026.101323","DOIUrl":"10.1016/j.onehlt.2026.101323","url":null,"abstract":"<div><div><em>Echinococcus</em> species are zoonotic cestodes found worldwide, posing a risk to humans and animals. <em>Echinococcus multilocularis</em> and <em>E. granulosus</em> sensu lato (s.l.) are both a concern in North America. Wild and domestic canids are definitive hosts, while rodents and ruminants are intermediate hosts for <em>E. multilocularis</em> and <em>E. granulosus</em> s.l., respectively. Humans can be alternative intermediate hosts for both <em>Echinococcus</em> species, and domestic dogs can serve as intermediate hosts for <em>E. multilocularis</em>. Recent <em>Echinococcus</em> detections in the eastern United States and Canada indicate a need for surveillance for these parasites. In total, we tested 27,293 fecal samples: pet dogs (<em>n</em> = 26,979), hunting dogs (<em>n</em> = 52), and wild canids (<em>n</em> = 262, including coyotes (<em>Canis latrans</em>), red fox (<em>Vulpes vulpes</em>), and gray fox (<em>Urocyon cinereoargenteus</em>)) from Pennsylvania, USA using the KeyScreen™ GI Parasite PCR. This PCR can detect and differentiate <em>E. multilocularis, E. granulosus</em> s.l., and <em>Taenia</em> spp. Infection with <em>Echinococcus multilocularis</em> was detected in four individuals: two gray foxes from Erie and Clearfield counties and two coyotes from Lackawanna and Susquehanna counties. Additionally, one coyote was positive from Wyoming county for <em>E. canadensis</em> (G8), an <em>E. granulosus</em> s.l. species. No pet dogs or hunting dogs were positive for either <em>Echinococcus</em> species. Wild canids from multiple counties were positive for <em>Taenia</em> species, but no domestic pet dogs or hunting dogs were positive. These findings expand the known range of <em>Echinococcus</em> species in Pennsylvania wild canids and demonstrate that KeyScreen™ is a valuable and rapid tool for detecting <em>Echinococcus</em> spp. in canid fecal samples.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"22 ","pages":"Article 101323"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145927017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic diversity and occurrence of antibiotic resistance genes from community wastewater in Dhaka and Cox's Bazar, Bangladesh","authors":"Akash Saha ,&nbsp;Rashedul Hasan ,&nbsp;Jenifar Quaiyum Ami ,&nbsp;Md Mahmudul Hasan ,&nbsp;Nuhu Amin ,&nbsp;Rehnuma Haque ,&nbsp;Mahbubur Rahman ,&nbsp;Farjana Jahan ,&nbsp;Rezaul Hasan ,&nbsp;Pengbo Liu ,&nbsp;Christine L. Moe ,&nbsp;Mohammad Enayet Hossain ,&nbsp;Mohammed Ziaur Rahman","doi":"10.1016/j.onehlt.2025.101301","DOIUrl":"10.1016/j.onehlt.2025.101301","url":null,"abstract":"<div><h3>Objectives</h3><div>Antibiotic resistance genes (ARGs), one of the key drivers of the growing antibiotic resistance, present a formidable public health concern. Wastewater may act as a key repository for both antibiotic-resistant bacteria and ARGs, facilitating their transfer across microbial communities. Given the limited knowledge of clinically relevant ARGs in community wastewater of Bangladesh, this study explored the genetic diversity and occurrence of ARGs from community wastewater discharges using a culture-independent approach.</div></div><div><h3>Methods</h3><div>Wastewater samples (<em>n</em> = 70) were collected from different sites in Dhaka and Cox's Bazar between April and May 2023. These samples underwent Nanotrap®-based culture-independent microbial concentration, followed by nucleic acid extraction and purification. Nine clinically important ARGs (<em>blaTEM</em>, <em>blaCTX-M</em>, <em>blaSHV</em>, <em>blaKPC</em>, <em>blaNDM</em>, <em>blaOXA</em>, <em>mcr-1</em>, <em>tolC</em>, and <em>acrA</em>) were detected using uniplex polymerase chain reaction (PCR), followed by Sanger sequencing. Bioinformatic analyses further explored the genetic diversity of the ARGs, as well as mutational analysis and molecular docking of <em>Klebsiella pneumoniae</em> carbapenemase (KPC).</div></div><div><h3>Results</h3><div><em>blaTEM</em>, <em>blaCTX-M</em>, <em>blaOXA</em>, <em>tolC</em>, and <em>acrA</em> were the most frequently detected ARGs (90 % each), followed by <em>blaNDM</em> (85.7 %), <em>blaKPC</em> (84.3 %), <em>blaSHV</em> (77.1 %), and <em>mcr-1</em> (52.9 %). The frequency of all ARGs was higher in Cox's Bazar compared to Dhaka, except for <em>mcr-1</em>. Co-occurrences of all nine ARGs were observed in 47.1 % of samples. Mutational analysis of KPC revealed unique missense mutations, and further docking demonstrated altered binding affinities and interactions between KPC-2 mutants and β-lactam antibiotics.</div></div><div><h3>Conclusion</h3><div>Our study provides the baseline data of clinically significant ARGs in local community wastewater in Bangladesh, reflecting the need for further surveillance to identify hotspots and guide antibiotic stewardship and wastewater management policies.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"22 ","pages":"Article 101301"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145927062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}