One Health最新文献

{"title":"Profiles and predictors of access to human and veterinary healthcare in multispecies households","authors":"Jennifer W. Applebaum ,&nbsp;Courtney Dunn ,&nbsp;Shelby E. McDonald ,&nbsp;Kaylinn Escobar ,&nbsp;Erin K. King ,&nbsp;Rosalie Corona ,&nbsp;Megan K. Mueller","doi":"10.1016/j.onehlt.2025.100981","DOIUrl":"10.1016/j.onehlt.2025.100981","url":null,"abstract":"<div><div>This study extends a behavioral-ecological framework for healthcare access and utilization to explore patterns of healthcare and veterinary care access within pet-owning households in the United States. Using Latent Class Analysis, a person-centered analytic approach, we identified five subgroups of pet owners in a diverse national sample (<em>n</em> = 750), each characterized by unique patterns of perceived access and actual usage of both human and veterinary healthcare. The first subgroup, “Good access/unfair system” (27%) and the second subgroup, “Good access/fair system” (30%) reported high probabilities of good healthcare and veterinary care access and limited financial burden but differed in their perceptions of healthcare fairness. The third subgroup, “Good access/Medicare” (14%), primarily comprised of older adults with Medicare, reported good access to both human and veterinary care and limited financial burden, underscoring Medicare's role in stable healthcare access. The fourth subgroup, “Moderate access” (11%), characterized by financial barriers despite non-employer health insurance, showed a lower likelihood of recent dental and veterinary visits. The fifth subgroup, “Poor access” (18%), with the poorest healthcare access and highest probability of Medicaid or no insurance, highlighted significant inequalities in healthcare and veterinary care access. Sociodemographic and social environmental factors were associated with subgroup membership. For example, the groups with better access were likely to have low financial fragility (Good access/unfair system OR = 4.61, <em>p</em> <em>&lt;</em> 0.001), and those with poorer access were unlikely (Poor access OR = 0.14). Additionally, the groups with better access were less likely to experience discrimination (Good access/fair system OR = 0.58, <em>p</em> <em>&lt;</em> 0.001) and those with poorer access were more likely (Moderate and Poor access both ORs = 1.37, <em>p</em> <em>&lt;</em> 0.001). These findings emphasize the need for policies addressing factors such as economic inequality and discrimination to improve healthcare and veterinary care access and utilization. Tailored interventions at individual and systemic levels are also suggested (i.e., the Colorado “Peticaid” proposal and the Seattle One Health Clinic) to mitigate disparities and enhance healthcare and veterinary care access for vulnerable populations.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 100981"},"PeriodicalIF":4.1,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143175757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-phenolic glycolipid antibodies in Mycobacterium bovis infected cattle","authors":"Zijie Zhou ,&nbsp;Anouk van Hooij ,&nbsp;J. Hessel M. van Dijk ,&nbsp;Nina Musch ,&nbsp;Louise Pierneef ,&nbsp;Hamza Khalid ,&nbsp;Kees Franken ,&nbsp;Thomas Holder ,&nbsp;Neil Watt ,&nbsp;Anita L. Michel ,&nbsp;Jeroen D.C. Codée ,&nbsp;Martin Vordermeier ,&nbsp;Paul L.A.M. Corstjens ,&nbsp;Elisabeth M.D.L. van der Heijden ,&nbsp;Jayne C. Hope ,&nbsp;Annemieke Geluk","doi":"10.1016/j.onehlt.2025.100982","DOIUrl":"10.1016/j.onehlt.2025.100982","url":null,"abstract":"<div><div><em>Mycobacterium bovis</em>, the causative agent of bovine tuberculosis (bTB), causes significant financial losses in the agricultural industry. Additionally, <em>M. bovis</em> transmission from animals to humans can result in zoonotic TB, especially in low- and middle-income countries (LMICs), highlighting the need to enhance One Health surveillance to mitigate this threat.</div><div>Antibodies directed against a major mycobacterial cell wall component of <em>M. leprae</em>, phenolic glycolipid-I (PGL-I), have shown excellent performance in identifying <em>M. leprae</em> infection in humans and animals. In this study, we therefore investigated whether antibodies against <em>M. bovis</em> PGL similarly represent a useful biomarker for <em>M. bovis</em> infection in cattle.</div><div>Comparing sera from naturally <em>M. bovis</em>-infected and the single intradermal comparative cervical tuberculin test (SICCT)-negative cattle, we assessed the potential of <em>M. bovis</em> PGL antibodies to identify this mycobacterial infection. Our results show that serum levels of anti-<em>M. bovis</em> PGL IgG and -IgM in <em>M. bovis</em>-infected cattle were significantly higher than in the SICCT-negative cattle. The sensitivity for anti-<em>M. bovis</em> PGL IgM in infected animals was, however, moderate (44.9 %) and the false-positive rate was 6.3 % in SICCT-negative cattle. Notably, vaccination with BCG- or heat-killed <em>M. bovis</em> did not affect serum levels of anti-<em>M. bovis</em> PGL IgM in cattle. Moreover, none of the 57 anti-<em>M. bovis</em> PGL-seropositive cattle tested positive in the anti-<em>M. leprae</em> PGL-I assay. This study shows for the first time that anti <em>M. bovis</em> PGL antibodies can be detected in infected cattle: anti-<em>M. bovis</em> PGL IgM is a highly specific, but moderately sensitive biomarker for <em>M. bovis</em> infection in cattle, showing potential for differentiate infected from vaccinated animals (DIVA). It could be a valuable component in a multi-biomarker approach for diagnosing bTB.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 100982"},"PeriodicalIF":4.1,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143175756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shaping the human gut microbiota: The role of canine companionship, lifestyle choices, and Blastocystis sp.","authors":"Zuzana Pavlíčková ,&nbsp;Barbora Pafčo ,&nbsp;Vladislav Ilík ,&nbsp;Lee O'Brien Andersen ,&nbsp;Milan Jirků ,&nbsp;Kristýna Brožová ,&nbsp;David Modrý ,&nbsp;Oldřiška Kadlecová ,&nbsp;Christen Rune Stensvold ,&nbsp;Kateřina Jirků","doi":"10.1016/j.onehlt.2025.100979","DOIUrl":"10.1016/j.onehlt.2025.100979","url":null,"abstract":"<div><div>External factors affecting composition of the human gut microbiota have attracted considerable attention in recent years. Among these factors, habitat sharing with other humans and companion animals, especially dogs, is considered crucial together with the presence of intestinal protists. The Czech Republic, known for one of the highest rates of dog ownership in Europe, provides an ideal setting for studying such relationships.</div><div>Here, we investigated the impact of dog ownership and lifestyle factors (residing in cities versus villages) on the gut microbiota (specifically bacteriome). In addition, we also investigated the influence of the common gut protist <em>Blastocystis</em> sp. on the human gut microbiota. Fecal DNAs from 118 humans and 54 dogs were subject to 16S rRNA gene sequencing using the Illumina MiSeq platform.</div><div>Greater microbial diversity was observed in humans than in dogs. Owning a dog had no significant effect on the alpha and beta diversity of the human microbiota, although some bacterial genera were enriched in dog owners. In relation to lifestyle, urban dwellers had higher levels of <em>Akkermansia</em>, while people living in villages had a more diverse gut microbiota. The presence of <em>Blastocystis</em> sp. in humans correlated with specific microbial patterns, indicating an important role for this micro-eukaryote in the gut ecosystem.</div><div>These findings highlight the intricate relationship between specific factors and the gut microbiota composition and emphasize the need for more extensive research in this area.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 100979"},"PeriodicalIF":4.1,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143175755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A persona-based exploration of rabies post-exposure prophylaxis seeking behavior and its implication for communication strategic planning: Evidence from Thailand","authors":"Jitjayang Yamabhai ,&nbsp;Patoo Cusripituck ,&nbsp;Teerawan Mingbualuang ,&nbsp;Nareerat Sangkachai ,&nbsp;Wimwiga Sakchainanon ,&nbsp;Chanatda Tungwongjulaniam ,&nbsp;Onphirul Yurachai ,&nbsp;Ratana Theerawat ,&nbsp;Anuwat Wiratsudakul","doi":"10.1016/j.onehlt.2025.100980","DOIUrl":"10.1016/j.onehlt.2025.100980","url":null,"abstract":"<div><div>Rabies is a lethal zoonotic illness that claims over 59,000 lives annually. However, this fatality can be avoided by postexposure prophylaxis (PEP). This study aimed to identify and characterize different personas of individuals regarding their PEP-seeking behavior and develop tailored communication strategies to encourage PEP adoption among these distinct groups effectively. We categorized our subjects, residing in two districts of Chonburi province, Thailand, into three groups: (i) individuals with a history of dog bites who underwent PEP; (ii) individuals bitten by dogs who did not receive PEP; and (iii) individuals who had never been bitten. Subsequently, we employed an empathy map, a visual method, and a customer journey map to better understand the participants' experiences and perceptions. A total of 38 individuals were interviewed. We categorized the participants into three distinct personas: positive, neutral, and negative trends. Individuals classified within the positive trend strongly advocate for seeking rabies vaccines in the event of a dog bite. Meanwhile, individuals who have a neutral inclination are more likely to contemplate getting vaccinated following a dog bite, particularly if the injury is substantial. Those with a negative trend demonstrate a notable lack of attention or concern toward preventing rabies. A lack of attention to the potential severity of the issue characterizes their attitude. Notably, nearly half (44.74 %; 17/38) of the individuals involved in the study indicated utilizing interpersonal communication, followed by digital platforms (42.11 %; 16/38) and traditional communication channels (10.52 %; 5/38). Tailoring communication modalities to suit each specific group is crucial for effective outreach.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 100980"},"PeriodicalIF":4.1,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143175759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Best practices for the experimental design of one health studies on companion animal and owner microbiomes – From data collection to analysis","authors":"Suzanne B. Clougher ,&nbsp;Dagmara Niedziela ,&nbsp;Piera Versura ,&nbsp;Grace Mulcahy","doi":"10.1016/j.onehlt.2025.100977","DOIUrl":"10.1016/j.onehlt.2025.100977","url":null,"abstract":"<div><div>The relationship between owner and companion animal represents an underestimated opportunity for the studying of One Health relationships between humans, animals, and the environment they share. Microbiome exchanges between owner and pet have been documented for the gut, skin, oral, and nasal microbiomes. These studies give a unique insight into bacterial flows between humans and animals, but come with their specific challenges.</div><div>This review discusses the data and sample collection challenges, as well as laboratory, bioinformatic and data analysis challenges specific to One Health studies on companion animal and owner microbiomes. We provide an overview of possible data to be collected and pitfalls to avoid during sample collection and conservation, DNA extraction, and library preparation. We present the main bioinformatics pipelines in sequencing-data microbiome analysis, as well as data analysis specific to pet-owner microbiome comparison. We review and compare three beta-diversity measures (Bray-Curtis dissimilarity, unweighted, and weighted UniFrac distances) for pet-owner distances and the tests to compare them. Finally, we propose a framework with key considerations to bear in mind when designing and carrying out owner-companion animal studies, as well as best practices to implement them.</div><div>Although these studies come with additional difficulties compared to species-specific microbiome studies, they offer the opportunity to identify biomarkers, environmental triggers, and impacts of pet-owner interactions across species.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 100977"},"PeriodicalIF":4.1,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143173488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mass rabies exposure of veterinary health care workers in Germany: Management, immune response, and tolerability of post exposure-prophylaxis","authors":"Lennart Lemmermann ,&nbsp;Jonathan Remppis ,&nbsp;Sabine Bélard ,&nbsp;Florian Steiner","doi":"10.1016/j.onehlt.2025.100978","DOIUrl":"10.1016/j.onehlt.2025.100978","url":null,"abstract":"<div><h3>Background</h3><div>Rabies is a fatal zoonotic disease caused by <em>Rabies lyssavirus</em>, primarily transmitted through dog bites. In 2008, Germany was declared free from terrestrial rabies by the WOAH. However, illegal pet imports can still lead to rabies exposure, as seen in the 2021 case of a rabid puppy illegally imported to Germany, resulting in a mass exposure incident.</div></div><div><h3>Methods</h3><div>This retrospective study assessed the post-exposure management of 39 veterinary clinic staff exposed to the rabid puppy, focusing on the practicability, immunogenicity, and tolerability of post-exposure prophylaxis (PEP). Data was collected from patient records and for follow-up by phone using standardized case record forms, entered into an Excel database, and analyzed descriptively. Ethics approval was obtained from the Ethics Committee of the University of Tübingen (8452022BO2).</div></div><div><h3>Results</h3><div>Exposed individuals received varying PEP regimens based on vaccination status and exposure level, including possible aerosol exposure. Most followed the Essen-Scheme, with some receiving human rabies immune globulin (hRIG). All developed protective antibody titers, and adverse reactions were generally mild.</div></div><div><h3>Conclusion</h3><div>This case highlights challenges in rabies mass exposure management and emphasizes needs for continued preparedness, resource allocation and education about rabies, even in regions considered rabies-free.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 100978"},"PeriodicalIF":4.1,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143175758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knowledge, attitudes, and practices of Native Americans in northern California regarding ticks and tick-borne diseases","authors":"Patricia Torres ,&nbsp;Elizabeth F. Pienaar ,&nbsp;Michelle A. Ritchie ,&nbsp;Mourad W. Gabriel ,&nbsp;Michael J. Yabsley","doi":"10.1016/j.onehlt.2025.100976","DOIUrl":"10.1016/j.onehlt.2025.100976","url":null,"abstract":"<div><div>Reports of tick-borne diseases (TBDs) are increasing worldwide, particularly in North America where a diversity of endemic and exotic tick species and pathogens occur. Native American populations have unique outdoor cultural and occupational practices that may impact their exposure to ticks, yet this risk remains understudied in the context of TBD. To address this gap, we examined knowledge, attitudes, and practices regarding ticks and TBDs among Native American communities in Humboldt County, California. We conducted semi-structured interviews with participants, who represented various tribes, at a cultural gathering. Cultural practices intertwined closely with outdoor activities (e.g., ceremonies, dances), potentially influencing local tick exposure patterns. Most research participants had been bitten by ticks and reported tick exposure by children and pets. Research participants demonstrated low knowledge about ticks and TBDs, as well as low levels of risk perceptions pertaining to TBDs. Research participants most commonly conducted tick checks after outdoor activity, wore long-sleeved clothing outdoors, and used homeopathic remedies or essential oils to prevent exposure to ticks and TBDs. Culturally appropriate outreach and education initiatives are needed to address TBD risk among Native American communities. Our study lays the groundwork for future research on the intersection of cultural practices and tick exposure, with implications for public health interventions that are tailored to the needs of indigenous populations.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 100976"},"PeriodicalIF":4.1,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11787543/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a zoonotic influenza distribution assessment and ranking system (ZIDAR): Technical application in Nepal to support cross-sectoral risk-based surveillance","authors":"Adam Charette-Castonguay ,&nbsp;Dipendra Gautam ,&nbsp;Binay Shrestha ,&nbsp;Hemant Chandra Ojha ,&nbsp;Barun Kumar Sharma ,&nbsp;Mukul Upadhayaya ,&nbsp;Sujan Rana ,&nbsp;Roshika Shrestha ,&nbsp;Lok Bandu Chaudhary ,&nbsp;Bhawana Kandel ,&nbsp;Rudra Prasad Marasini ,&nbsp;Sharmila Chapagain ,&nbsp;Tulsi Ram Gompo ,&nbsp;Surendra Karki ,&nbsp;Apsara Poudel ,&nbsp;Saugat Shrestha ,&nbsp;Avinash Sunny Kayastha ,&nbsp;Arun Kumar Govindakarnavar ,&nbsp;Reuben Samuel ,&nbsp;Allison Gocotano ,&nbsp;Ricardo J. Soares Magalhães","doi":"10.1016/j.onehlt.2025.100975","DOIUrl":"10.1016/j.onehlt.2025.100975","url":null,"abstract":"<div><div>Zoonotic influenza poses a significant public health concern to agricultural industries, food security, wildlife conservation, and human health. Nations situated along migratory bird flyways and characterised by dense populations of livestock and humans, and low biosecurity of production animal value chains are particularly vulnerable to zoonotic influenza outbreaks. While spatial risk assessments have been used to map vulnerable areas, their applicability across multiple sectors has been so far limited. Here, we introduce the development and application of a Zoonotic Influenza Distribution and Ranking (ZIDAR) framework to identify areas highly suitable for zoonotic influenza transmission across multiple exposure interfaces and to measure the importance of associated risk factors. The development of ZIDAR involves a seven-step approach distributed across an initial expert consultation stage followed by a technical modelling stage. The expert consultation stage aims to define interfaces of exposure across human, livestock and wildlife, identification of associated risk factors for each of the identified interfaces and a prioritisation activity to define weights for the interfaces and associated risk factors. This is then followed by a technical phase involving model building, model structure validation, data gathering and assessment of model performance. The model development and performance assessment steps of the technical stage includes a model calibration step to maximise model fitness with regards to wildlife and animal interfaces by finding pareto-efficient sets of weights for risk factors. We applied the ZIDAR framework in Nepal and the resulting model structure enabled the identification of hotspot areas where the risk of transmission is more significant across multiple interfaces simultaneously. The ZIDAR Nepal model's predictive accuracy, determined by the area under the receiver operating characteristic curve, demonstrated strong performance: 0.87 and 0.85 for the wildlife and animal components, respectively. The ZIDAR framework presented here provides valuable insights to enable the formulation of comprehensive One Health surveillance programs and inform targeted and effective interventions to bolster pandemic preparedness strategies.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 100975"},"PeriodicalIF":4.1,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143174832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular assessment of oyster microbiomes and viromes reveals their potential as pathogen and ecological sentinels","authors":"Jordan R. Walker ,&nbsp;Dennis A. Bente ,&nbsp;Megan T. Burch ,&nbsp;Filipe M. Cerqueira ,&nbsp;Ping Ren ,&nbsp;Jessica M. Labonté","doi":"10.1016/j.onehlt.2025.100973","DOIUrl":"10.1016/j.onehlt.2025.100973","url":null,"abstract":"<div><div>Oyster aquaculture world-wide is a booming industry that can provide many benefits to coastal habitats, including economic, ecosystem-level, and cultural benefits. Oysters present several risks for human consumption, including transmission of parasites, and bacterial and viral pathogens. Oyster microbiomes are well-defined, but their connection to the incidence of pathogens, humans or others, is unclear. Furthermore, viruses associated with oysters are largely unknown, and their connection to humans, animals, and ecosystem health has not been explored. Here, we employed a One Health framework and modern molecular techniques, including 16S rRNA amplicon and metagenomic sequencing, to identify links between changes in the microbial and viral communities associated with oysters and the incidence of pathogens detected in oyster tissues and their surrounding environments. In addition, we adapted the BioFire® FilmArray®, commonly used in hospitals, to determine the presence of human pathogens within the sampled oysters. We detected known human pathogens in 50 % of the oysters tested. Within the genomic datasets, we noted that pathogens of humans, animals, and plants in oysters were shared with the nearby water and sediments, suggesting a sink–source dynamic between the oysters and their surroundings. 16S rRNA gene analysis revealed that while oysters share common microbial constituents with their surrounding environments, they enrich for certain bacteria such as Mycoplasmatales, Fusobacteriales, and Spirochaetales. On the contrary, we found that oyster viromes harbored the same viruses in near equal relative abundances as their surrounding environments. Our results show how oysters could be used not only to determine the risk of human pathogens within coastal estuaries but also how oyster viruses could be used as ecosystem-level sentinels.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 100973"},"PeriodicalIF":4.1,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11786891/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monitoring for respiratory viruses among wild canids, Texas","authors":"Lyudmyla V. Marushchak ,&nbsp;Laura A. Pulscher ,&nbsp;Judith U. Oguzie ,&nbsp;Diego B. Silva ,&nbsp;Kenneth A. Waldrup ,&nbsp;Douglas M. Watts ,&nbsp;Gregory C. Gray","doi":"10.1016/j.onehlt.2025.100974","DOIUrl":"10.1016/j.onehlt.2025.100974","url":null,"abstract":"<div><div>The cross-species transmission of respiratory viruses such as SARS-CoV-2 and avian influenza underscores the need for novel respiratory virus surveillance at the human-animal interface. In this 2023 pilot study we examined oral and rectal swab samples from 15 deceased wild animals for novel respiratory viruses. We used virus molecular techniques, culture and next-generation nucleotide sequencing to search for and characterize viruses in the <em>Coronaviridae</em> and <em>Orthomyxoviridae</em> families. Through these activities we detected and characterized one canine coronaviruses (CCoVs) each from a gray fox (<em>Urocyon cinereorgenteus</em>) and a feral dog (<em>Canis lupus familiaris</em>). The gray fox CCoV sequence clustered with other CCoVs reported in other canids from other regions of the world. The feral dog CCoV sequence was closely related to CCoVs reported in Brazil and the United Kingdom. This pilot study demonstrated the usefulness of a noninvasive monitoring approach in detecting and characterizing respiratory viruses among wild canids.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 100974"},"PeriodicalIF":4.1,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11787532/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}