One Health最新文献

{"title":"Predicting the spatio-temporal risk of human tick-borne encephalitis (TBE) in Europe by combining hazard and exposure drivers","authors":"Francesca Dagostin ,&nbsp;Diana Erazo ,&nbsp;Giovanni Marini ,&nbsp;Daniele Da Re ,&nbsp;Valentina Tagliapietra ,&nbsp;Maria Avdicova ,&nbsp;Tatjana Avšič-Županc ,&nbsp;Timothée Dub ,&nbsp;Nahuel Fiorito ,&nbsp;Nataša Knap ,&nbsp;Céline M. Gossner ,&nbsp;Jana Kerlik ,&nbsp;Henna Mäkelä ,&nbsp;Mateusz Markowicz ,&nbsp;Roya Olyazadeh ,&nbsp;Lukas Richter ,&nbsp;William Wint ,&nbsp;Maria Grazia Zuccali ,&nbsp;Milda Žygutienė ,&nbsp;Simon Dellicour ,&nbsp;Annapaola Rizzoli","doi":"10.1016/j.onehlt.2026.101331","DOIUrl":"10.1016/j.onehlt.2026.101331","url":null,"abstract":"<div><h3>Background</h3><div>Tick-borne encephalitis (TBE), caused by tick-borne encephalitis virus (TBEV), is a zoonotic disease that can lead to severe neurological symptoms. Given the increasing number of reported human TBE cases in Europe, we developed a spatio-temporal predictive model to infer the year-to-year probability of human TBE occurrence across Europe at the regional and municipal administrative levels.</div></div><div><h3>Methods</h3><div>We derived the distribution of human TBE cases at the regional level during 2017–2022 by using data provided by the European Centre for Disease Prevention and Control (ECDC), and at the municipal level by using data provided by Austria, Finland, Italy, Lithuania, and Slovakia. We modeled the probability of presence of human TBE cases at the regional and municipal levels for the period 2017–2025 with a boosted regression trees model, including covariates that affect both the natural hazard of virus circulation and human exposure to tick bites.</div></div><div><h3>Findings</h3><div>Areas with the highest probability of human TBE infections are located in central-eastern Europe, the Baltic states, and along the coastline of Nordic countries. Our results highlight a statistically significant rising trend in human TBE risk not only in north-western, but also in south-western European countries. Such areas are characterised by the presence of key tick host species, forested areas, intense human activity in forests, steep drops in late summer temperatures and high precipitation amounts during the driest months. The model showed good predictive performance, with a mean AUC of 0.84 (SD = 0.03), sensitivity of 0.83 (SD = 0.01), and specificity of 0.80 (SD = 0.01) at the regional level, and a mean AUC of 0.82 (SD = 0.03), sensitivity of 0.83 (SD = 0.01), and specificity of 0.69 (SD = 0.01) at the municipal level.</div></div><div><h3>Interpretation</h3><div>With ongoing climate and land use changes, the number of human TBE cases is likely to increase and spread into new areas. This highlights the importance of predictive models that can identify potential risk areas to support disease prevention and control efforts by public health authorities. The approach adopted, by fitting a One Health framework and leveraging lagged covaries, enables timely one-year-ahead predictions and enhances our current understanding of TBE risk under a global change scenario.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"22 ","pages":"Article 101331"},"PeriodicalIF":4.5,"publicationDate":"2026-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145977279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Key aspects of One Health governance in the European Union","authors":"Fergal Donnelly ,&nbsp;Nikolaos I. Stilianakis","doi":"10.1016/j.onehlt.2026.101325","DOIUrl":"10.1016/j.onehlt.2026.101325","url":null,"abstract":"<div><div>One Health governance involves the integrated coordination and management of policies, programs, and activities addressing the interconnected health of humans, animals, and the environment. Its development and implementation necessitate collaboration and coordination across various sectors and require significant resources. Consequently, key performance indicators are essential for monitoring progress and informing adaptations. Additionally, integrative surveillance systems encompassing human, animal, and environmental health sources, along with digital technologies and interoperable data systems, are crucial components for effective governance. These elements collectively foster health and well-being in a holistic manner.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"22 ","pages":"Article 101325"},"PeriodicalIF":4.5,"publicationDate":"2026-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145977198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Turning urban wildlife mortality into a surveillance tool: Detection of vector-borne pathogens in carcasses of hedgehogs, squirrels, and blackbirds","authors":"Karolina Volfová ,&nbsp;Václav Hönig ,&nbsp;Michal Houda ,&nbsp;Petr Papežík ,&nbsp;Paulina Maria Lesiczka ,&nbsp;Manoj Fonville ,&nbsp;Hein Sprong ,&nbsp;Barbora Černá Bolfíková ,&nbsp;Pavel Hulva ,&nbsp;Daniel Růžek ,&nbsp;Lada Hofmannová ,&nbsp;Jan Votýpka ,&nbsp;David Modrý","doi":"10.1016/j.onehlt.2026.101328","DOIUrl":"10.1016/j.onehlt.2026.101328","url":null,"abstract":"<div><div>Tick-borne zoonoses pose a major challenge to human and animal health, driving efforts to monitor the distribution, intensity, and diversity of their causative agents. Within the One Health framework, which links human, animal, and environmental health, integrated surveillance strategies are increasingly needed. However, most studies focus on tick vectors, while vertebrate reservoirs are often overlooked due to labour-intensive sampling, the need for specialized skills, and legislative or species protection constraints.</div><div>This study evaluated whether carcasses of accidentally killed wildlife (primarily roadkill) can serve as a source of biological material for vector-borne pathogen surveillance, with a focus on urban habitats due to their public health relevance. Hedgehogs, squirrels, and blackbirds were selected as synanthropic species that thrive in cities, are commonly infested by ticks, and act as hosts for zoonotic tick-borne pathogens (TBPs).</div><div>A total of 268 carcasses (125 hedgehogs, 55 squirrels, and 88 blackbirds) were collected across multiple Czech cities with public assistance. Overall, 1836 tissue samples were analyzed using multiplex real-time PCR assays targeting over ten microorganisms. Detection efficiency was compared across tissues, with ear and skin consistently the most reliable and versatile sample types. Individual pathogen-host-tissue combinations reached 65–93% efficiency, highlighting the value of multi-tissue sampling. The most prevalent TBPs detected were <em>Anaplasma phagocytophilum</em>, <em>Borrelia burgdorferi</em> s.l., <em>Bartonella</em> spp., and <em>Rickettsia helvetica</em>.</div><div>In conclusion, carcasses of accidentally killed urban wildlife provide a practical and valuable resource for TBP surveillance, complementing vector-focused methods. This approach supports One Health principles by integrating wildlife monitoring into urban disease surveillance efforts.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"22 ","pages":"Article 101328"},"PeriodicalIF":4.5,"publicationDate":"2026-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145977199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The ecology of biting: buzzing through the main ecological, environmental and biological drivers of mosquito-borne diseases","authors":"Elisa Fesce ,&nbsp;Josué Martínez-de la Puente ,&nbsp;Martina Ferraguti","doi":"10.1016/j.onehlt.2026.101326","DOIUrl":"10.1016/j.onehlt.2026.101326","url":null,"abstract":"<div><div>Mosquito populations are shaped by a variety of environmental drivers, including temperature fluctuations, habitat alterations, and physicochemical factors. These drivers impact mosquito community composition, influencing the spread of vector-borne diseases. Species differ in their sensitivity to environmental changes, with some thriving in anthropogenic landscapes and others exhibit preferences for natural habitats. Abiotic factors such as temperature, water pH, salinity, and dissolved oxygen strongly affect larval survival and development, while interspecific competition among larvae shapes community structure and species abundance, impacting pathogen transmission. Mosquito feeding preferences further influence pathogen transmission by determining host selection; with opportunistic mosquito species that can act as bridge vectors between humans, domestic animals, and wildlife, facilitating the spread of zoonotic pathogens. In this respect, understanding the dynamics of zoonotic pathogens requires a One Health approach that integrates human, animal and environmental health. Mathematical models, in particular, draw on ecological, environmental and biological factors to elucidate mosquito population dynamics and disease transmission, reinforcing the importance of adopting an integrated perspective. We examine the key environmental, ecological, and biological factors shaping mosquito community composition, and highlight the role of mathematical modelling in clarifying how these factors influence mosquito-borne disease transmission. Our findings emphasize that vector surveillance and control programs should target specific vector species in relevant habitats to optimize effectiveness and reduce economic costs.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"22 ","pages":"Article 101326"},"PeriodicalIF":4.5,"publicationDate":"2026-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145977200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"One health and community health workers: Bridging the gap in global health resilience","authors":"Claude Mambo Muvunyi ,&nbsp;Pierre Gashema ,&nbsp;Emmanuel Edwar Siddig ,&nbsp;Jean de Dieu Harelimana ,&nbsp;Jean Kaseya","doi":"10.1016/j.onehlt.2026.101330","DOIUrl":"10.1016/j.onehlt.2026.101330","url":null,"abstract":"","PeriodicalId":19577,"journal":{"name":"One Health","volume":"22 ","pages":"Article 101330"},"PeriodicalIF":4.5,"publicationDate":"2026-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145977197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human 3D liver spheroids support productive infection of a novel tick-borne phenuivirus","authors":"Wenbo Xu ,&nbsp;Liyan Sui ,&nbsp;Nan Liu ,&nbsp;Lesley Bell-Sakyi ,&nbsp;Yicheng Zhao ,&nbsp;Yuanzhi Wang ,&nbsp;Yinghua Zhao ,&nbsp;Changfeng Zhu ,&nbsp;Quan Liu","doi":"10.1016/j.onehlt.2026.101321","DOIUrl":"10.1016/j.onehlt.2026.101321","url":null,"abstract":"<div><div>The identification of novel tick-borne viruses, such as Mukawa virus (MKWV), underscores a growing need to assess their potential public health risks. In this study, we isolated the MKWV strain HLJ1 from <em>Ixodes persulcatus</em> ticks. While this initial isolate demonstrated limited replication in mammalian cell lines and mice, it productively infected human primary cell-derived 3D spheroids. Serial passaging in this model significantly enhanced viral titers, suggesting adaptive evolution. The resulting adapted strain exhibited increased virulence, causing pronounced cytopathic effects in Vero cells, infecting diverse mammalian cell types, and leading to 100% mortality in suckling mice, with associated liver inflammation and damage. These pathogenic outcomes were recapitulated in the 3D human liver spheroids, which showed impaired cellular synthetic functions, cell death, and heightened inflammatory responses following infection. Epidemiological screening of 145 serum samples from tick-bitten patients in Northeastern China revealed low but detectable exposure, with 1.4% positive for MKWV RNA, 4.8% for IgG antibodies, and 3.4% for neutralizing antibodies. Collectively, our findings integrate a novel human-relevant 3D culture system with field surveillance to highlight the potential risks of MKWV to human health and provide a model framework for evaluating emerging tick-borne viruses.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"22 ","pages":"Article 101321"},"PeriodicalIF":4.5,"publicationDate":"2026-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145977280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rickettsial seropositivity in Lao PDR smallholder livestock farms: Implications for animal and human health","authors":"Chantal Tawfik ,&nbsp;James R. Young ,&nbsp;Syseng Khounsy ,&nbsp;Phouvong Phommachanh ,&nbsp;Peter Christensen ,&nbsp;Watthana Theppangna ,&nbsp;Tom Hughes ,&nbsp;Jantana Wongsantichon ,&nbsp;Stuart D. Blacksell ,&nbsp;Michael P. Ward","doi":"10.1016/j.onehlt.2026.101327","DOIUrl":"10.1016/j.onehlt.2026.101327","url":null,"abstract":"<div><div>Rickettsioses are emerging zoonotic diseases in Southeast Asia caused by vector-borne, intracellular <em>Rickettsia</em> bacteria that threaten public health, animal welfare, and food security. Despite their importance to rural livelihoods, the epidemiology of rickettsial exposure in livestock remains poorly understood. This study used abattoir-based serology to evaluate livestock as sentinels of environmental exposure to Rickettsia in Lao PDR and to identify spatial hotspots and risk factors for seropositivity. Abattoir-based serological data were generated from cattle, pigs, and water buffalo samples collected in 18 provinces between January 2022 and April 2023. The immunofluorescence assay was used to detect antibodies against three rickettsial antigens: Scrub Typhus Group (<em>Orientia tsutsugamushi</em>), Typhus Group (<em>R. typhi</em>), and Spotted Fever Group (<em>R. conorii</em>, <em>R. honei</em>). Samples with IgG titres ≥1:100 were considered positive. Of 821 samples tested, 32 were seropositive: 25 cattle (9.9%), seven pigs (2.4%), and none of the buffalo. Breed and age were significant predictors, with non-native breeds and animals under one year old more likely to be positive (<em>p</em> &lt; 0.05). Spatial-temporal analysis revealed one significant cluster in cattle (<em>p</em> = 0.0056) in southern Laos. These results represent the first nationwide serological assessment of Rickettsia exposure in Lao livestock.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"22 ","pages":"Article 101327"},"PeriodicalIF":4.5,"publicationDate":"2026-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145977281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated region-specific modeling of H5 avian influenza in Asia using ENSO-based forecasts","authors":"Yinghui Su ,&nbsp;Ruoxuan Wu ,&nbsp;Pengfei Liu ,&nbsp;Zhichao Li ,&nbsp;Juan Pu ,&nbsp;Lu Wang","doi":"10.1016/j.onehlt.2026.101322","DOIUrl":"10.1016/j.onehlt.2026.101322","url":null,"abstract":"<div><div>Highly pathogenic avian influenza (HPAI), particularly of the H5 subtype, remains a persistent threat to poultry, wildlife, and public health across Asia. This study quantifies the influence of the El Niño–Southern Oscillation (ENSO), using the Multivariate ENSO Index (MEI) as the primary predictor, on the climate-driven dynamics of H5 HPAI through region- and host-stratified generalized additive models (GAMs). Seven region–host strata across Asia were modeled separately, revealing pronounced heterogeneity in event frequency. A clear negative correlation with MEI was identified in domestic poultry across East and South Asia, where higher MEI values, corresponding to El Niño conditions, were linked to reduced event frequencies. In contrast, wild bird populations in East and South Asia displayed irregular, multimodal response patterns to MEI, suggesting phase-specific sensitivities to climate variability. A recurrent neural network (RNN) was further employed to forecast MEI trends, which were then incorporated into the GAMs to predict event dynamics. The forecasts highlighted continued epidemic pressure in East Asia's wild birds, in contrast to stable or declining trends elsewhere. Given the zoonotic potential of H5 viruses, these climate-informed risk forecasts could help inform timely interventions to prevent animal-to-human transmission and support integrated One Health preparedness frameworks. This integrative statistical–deep learning framework offers valuable support for short-term early warning and regionally targeted prevention strategies for H5 HPAI preparedness across Asia.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"22 ","pages":"Article 101322"},"PeriodicalIF":4.5,"publicationDate":"2026-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145927020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and distribution of Echinococcus species in domestic dogs and wild canids in Pennsylvania: KeyScreen® GI Parasite PCR testing of fecal samples","authors":"Kayla B. Garrett ,&nbsp;Justin Brown ,&nbsp;Pablo D. Jimenez Castro ,&nbsp;Michelle Evason ,&nbsp;Maria Jarque ,&nbsp;Emily Jenkins ,&nbsp;Noelle Kuhn ,&nbsp;Christian Leutenegger ,&nbsp;Cecilia Lozoya ,&nbsp;Samantha Loo ,&nbsp;Lauren Maxwell ,&nbsp;Kevin D. Niedringhaus ,&nbsp;Andrew S. Peregrine ,&nbsp;Christian Savard ,&nbsp;Jeffrey Tereski ,&nbsp;Taylor Volappi ,&nbsp;Kyle Van Why ,&nbsp;Michael J. Yabsley ,&nbsp;Christopher A. Cleveland","doi":"10.1016/j.onehlt.2026.101323","DOIUrl":"10.1016/j.onehlt.2026.101323","url":null,"abstract":"<div><div><em>Echinococcus</em> species are zoonotic cestodes found worldwide, posing a risk to humans and animals. <em>Echinococcus multilocularis</em> and <em>E. granulosus</em> sensu lato (s.l.) are both a concern in North America. Wild and domestic canids are definitive hosts, while rodents and ruminants are intermediate hosts for <em>E. multilocularis</em> and <em>E. granulosus</em> s.l., respectively. Humans can be alternative intermediate hosts for both <em>Echinococcus</em> species, and domestic dogs can serve as intermediate hosts for <em>E. multilocularis</em>. Recent <em>Echinococcus</em> detections in the eastern United States and Canada indicate a need for surveillance for these parasites. In total, we tested 27,293 fecal samples: pet dogs (<em>n</em> = 26,979), hunting dogs (<em>n</em> = 52), and wild canids (<em>n</em> = 262, including coyotes (<em>Canis latrans</em>), red fox (<em>Vulpes vulpes</em>), and gray fox (<em>Urocyon cinereoargenteus</em>)) from Pennsylvania, USA using the KeyScreen™ GI Parasite PCR. This PCR can detect and differentiate <em>E. multilocularis, E. granulosus</em> s.l., and <em>Taenia</em> spp. Infection with <em>Echinococcus multilocularis</em> was detected in four individuals: two gray foxes from Erie and Clearfield counties and two coyotes from Lackawanna and Susquehanna counties. Additionally, one coyote was positive from Wyoming county for <em>E. canadensis</em> (G8), an <em>E. granulosus</em> s.l. species. No pet dogs or hunting dogs were positive for either <em>Echinococcus</em> species. Wild canids from multiple counties were positive for <em>Taenia</em> species, but no domestic pet dogs or hunting dogs were positive. These findings expand the known range of <em>Echinococcus</em> species in Pennsylvania wild canids and demonstrate that KeyScreen™ is a valuable and rapid tool for detecting <em>Echinococcus</em> spp. in canid fecal samples.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"22 ","pages":"Article 101323"},"PeriodicalIF":4.5,"publicationDate":"2026-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145927017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Botucatu Document: One health antifungal resistance policies — A call for action","authors":"Paulo Cezar Ceresini ,&nbsp;Waldir Cintra de Jesus Junior ,&nbsp;Ana Carolina Firmino ,&nbsp;Bárbara Pereira Christofaro Silva ,&nbsp;Danilo Tancler Stipp ,&nbsp;Edson Luiz Furtado ,&nbsp;Fábio Luiz Checchio Mingotte ,&nbsp;Karine Assis Costa ,&nbsp;Paulo Renato Matos Lopes ,&nbsp;Rita Luiza Peruquetti","doi":"10.1016/j.onehlt.2026.101319","DOIUrl":"10.1016/j.onehlt.2026.101319","url":null,"abstract":"<div><div>Antifungal resistance in <em>Aspergillus fumigatus</em> and other environmental fungi represents a growing global threat to human health, driven in part by agricultural fungicide use. The scale of the threat is masked by inadequate multisector surveillance. In December 2025, the Brazilian Network Meeting on <em>Aspergillus fumigatus</em> Antimicrobial Resistance convened clinical, agricultural, environmental, and public-health experts to address these challenges using a One Health framework. Through a structured plenary deliberation, participants approved “<em>The Botucatu Document</em>: One Health Antifungal Resistance Policies — A Call for Action,” a unified Public Statement of Concern outlining governance principles and coordinated national actions for Brazil. The Statement reflects consensus across four Working Groups on clinical surveillance, environmental monitoring, fungicide regulation, and public health communication. Together, these directives call for independent AMR data collection, FAIR transparency, cross-sector governance, strengthened laboratory capacity, environmental aerobiome surveillance, new fungicide risk-assessment criteria, and comprehensive One Health communication strategies. This manuscript presents the full Public Statement as approved verbatim, situates it within global AMR policy frameworks, and highlights implications for Brazil's forthcoming National AMR Action Plan (2026–2031). The <em>Botucatu Document</em> represents a milestone in aligning agricultural and clinical sectors around a shared One Health AMR agenda.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"22 ","pages":"Article 101319"},"PeriodicalIF":4.5,"publicationDate":"2026-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145926321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}