Genome-wide identification of shaker K+ channel gene family in sugar beet (Beta vulgaris L.) and function of BvSKOR in response to salt and drought stresses

Abstract

Potassium (K⁺) is the most abundant cation in plants, which is absorbed by roots and distributed throughout the plants and within plant cells, and is involved in various cellular processes. Shaker K⁺ channel plays crucial roles in the absorption and distribution of K⁺ and in the response to abiotic stress in plants. Herein, a total of six shaker K⁺ channel genes, BvKAT1, BvKAT3, BvAKT1, BvAKT2, BvAKT5, and BvSKOR, were identified in the genome of sugar beet (Beta vulgaris L.). The coding domain sequences (CDS) of these genes ranged from 2232 to 2739 bp, and protein lengths were varied from 743 to 912 aa. The shaker K⁺ channel genes contained hormone-related and light responsiveness cis-acting regulatory elements. The phylogenetic analysis showed that BvSKOR was highly conserved and contained six transmembrane structures. The expression patterns of BvSKOR under salt and osmotic stress were analyzed by qRT-PCR, and found that the expression level of BvSKOR under low concentration salt and osmotic stress at short period of treatment were significantly higher than that of the control group. The function of BvSKOR was further verified in tobacco (Nicotiana tabacum), and the results showed that under salt and osmotic stress, the roots of transgenic plants were significantly stronger than those of wild type (WT) plants, and the relative water content (RWC), chlorophyll, proline, soluble sugar, soluble proteins contents and antioxidant enzyme activity were significantly higher than those of WT plants. These results indicated that overexpression of BvSKOR can significantly enhance the salt and drought tolerance in transgenic tobacco plants. This study could provide theoretical support and genetic resources for genetic improvement of crops stress resistance.