Simultaneous endogenous partial denitrification/anammox process for low-strength wastewater treatment: Process optimization, nitrogen removal and microbial dynamics

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biochemical Engineering Journal Pub Date : 2024-11-09 DOI:10.1016/j.bej.2024.109568

Dong Li , Fanxu Zeng , Songwei Yang , Yuliang Zhu , Zhu Li , Huiping Zeng , Jie Zhang

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Abstract

The endogenous partial denitrification process (EPD) led by glycogen-accumulating organisms (GAOs) has become an alternative to NO₂⁻ supply in mainstream anaerobic-ammonia oxidation (anammox). However, low autotrophic nitrogen removal contribution is an urgent problem that needs to be solved in simultaneous endogenous partial denitrification/anammox (EPDA) system. This study used anaerobic duration optimization to enhance the autotrophic nitrogen removal capacity of EPDA system. The results showed that the EPDA activity increased from 0.67 to 1.09 mg N/g VSS·h after anaerobic time was extended to 120 min. This significantly improved the contribution of anammox to TIN removal, increasing from 23.5 % to 61.6 %. During the phase Ⅲ, Eff.TIN of 4.5±1.8 mg/L and NRE of 92.2 %±3.0 %. The enrichment of AnAOB (Candidatus Brocadia) and GAOs (Defluviicoccus) was responsible for maintaining the stability of the EPDA process. This study provides a feasible optimization strategy for improving the contribution of autotrophic nitrogen removal in the EPDA system.

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用于低浓度废水处理的同步内源部分反硝化/氨氧化工艺：工艺优化、脱氮和微生物动力学

由糖原累积生物（GAOs）主导的内源部分反硝化过程（EPD）已成为主流厌氧氨氧化（anammox）过程中氮氧化物供应的替代方式。然而，在同时进行的内源部分反硝化/氨氧化（EPDA）系统中，自养脱氮贡献率低是一个亟待解决的问题。本研究利用厌氧时间优化来提高 EPDA 系统的自养脱氮能力。结果表明，厌氧时间延长到 120 分钟后，EPDA 活性从 0.67 mg N/g VSS-h 提高到 1.09 mg N/g VSS-h。这大大提高了anammox对TIN去除的贡献率，从23.5%提高到61.6%。在第Ⅲ阶段，Eff.TIN 为 4.5±1.8 mg/L，NRE 为 92.2 %±3.0 %。AnAOB（Candidatus Brocadia）和 GAOs（Defluviicoccus）的富集是维持 EPDA 过程稳定的原因。这项研究为提高 EPDA 系统自养脱氮的贡献率提供了可行的优化策略。

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来源期刊

Biochemical Engineering Journal 工程技术-工程：化工

CiteScore

7.10

自引率

5.10%

发文量

380

审稿时长

34 days

期刊介绍： The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology. The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields: Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics Biosensors and Biodevices including biofabrication and novel fuel cell development Bioseparations including scale-up and protein refolding/renaturation Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells Bioreactor Systems including characterization, optimization and scale-up Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis Protein Engineering including enzyme engineering and directed evolution.