A lysosome-targeted fluorescent probe with large Stokes shift for visualizing biothiols in vivo and in vitro

IF 4.6 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES iScience Pub Date : 2024-11-07 DOI:10.1016/j.isci.2024.111334

Taotao Zhao , Tong Zhang , Zijun Tao , Zhe Zhou , Xiaofeng Xia , Zhengjun Wu , Feiyi Wang , Jun Ren , Erfei Wang

引用次数: 0

Abstract

Lysosomal biothiols play critical roles in numerous cellular processes and diseases. Researching an effective method for real-time labeling biothiols in lysosomes is of great significance and urgency, as it could provide essential information for the diagnosis of relevant diseases. In this study, we developed a lysosome-targeted fluorescent probe (LY-DCM-P) with a large Stokes shift of 150 nm for the sensitive and selective detection of biothiols in vivo and in vitro. Additionally, LY-DCM-P showed low cytotoxicity and excellent lysosome-targeted ability. The probe was successfully employed to monitor fluctuations in lysosomal biothiols in various living systems, enabling enormous potential to accurately monitor the occurrence and progress of biothiol-related diseases.

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具有大斯托克斯偏移的溶酶体靶向荧光探针，用于在体内和体外观察生物硫醇

溶酶体生物硫醇在许多细胞过程和疾病中发挥着关键作用。研究一种实时标记溶酶体中生物硫醇的有效方法意义重大、迫在眉睫，因为它可以为相关疾病的诊断提供重要信息。在这项研究中，我们开发了一种溶酶体靶向荧光探针（LY-DCM-P），它具有 150 nm 的大斯托克斯位移，可在体内和体外灵敏、选择性地检测生物硫醇。此外，LY-DCM-P 显示出较低的细胞毒性和出色的溶酶体靶向能力。该探针被成功用于监测各种生命系统中溶酶体生物硫醇的波动，为准确监测生物硫醇相关疾病的发生和进展提供了巨大的潜力。

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来源期刊

iScience Multidisciplinary-Multidisciplinary

CiteScore

7.20

自引率

1.70%

发文量

1972

审稿时长

6 weeks

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