Antibacterial chitosan/organic rectorite nanocomposite-conjugated gelatin/β-cyclodextrin hydrogels with improved hemostasis performance for wound repair

IF 10.7 1区 化学 Q1 CHEMISTRY, APPLIED Carbohydrate Polymers Pub Date : 2024-11-13 DOI:10.1016/j.carbpol.2024.122961
Rong Huang , Yongqian Bian , Wenxuan Wang , Lirong Xu, Hao Zhang, Haowei Zhou, Jincheng Du, Jiaqi Li, Yuheng Zhang, Xueyong Li, Jing Li
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Abstract

Bacterial infections and severe bleeding continue to pose significant challenges in wound repair. There is an urgent need for innovative, nature-inspired hydrogel dressings with antibacterial and hemostatic properties. A Ge-β-CD-CS-OREC conjugate hydrogel was developed by grafting β-CD and CS-OREC nanocomposites into a Ge matrix using EDC/NHS crosslinking, as confirmed by FT-IR and EDX analyses. Compared to single Ge-β-CD cross-linked hydrogels, the addition of CS-OREC enhanced the hydrogel's properties, including increased pore size (60 ± 14 μm), improved wettability (WCA = 28.82 ± 0.6°), enhanced tensile strength (41.3 ± 3.56 KPa), and strong tissue adhesion. Furthermore, this hydrogel demonstrated excellent cytocompatibility when co-cultured with keratinocytes (Kcs) and vascular endothelial cells (VECs). The incorporation of CS chains into OREC interlayers allowed the hydrogel to specifically target bacteria and increase membrane permeability in Pseudomonas aeruginosa (PA), Klebsiella pneumoniae (KP), and Staphylococcus aureus (SA), effectively reducing the bacterial load in infected wounds by 50.24–73.92 % compared to controls in vivo. Further, the hydrogel exhibited superior hemostatic efficiency (78 ± 10 s) over commercial gauze and other gels by enhancing platelet activation and coagulation factor secretion. The hydrogel accelerated tissue regeneration by promoting epithelial maturation and blood vessel regeneration, indicating its clinical potential as promising wound dressing.
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具有更好止血性能的抗菌壳聚糖/有机雷克托石纳米复合-共轭明胶/β-环糊精水凝胶用于伤口修复
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来源期刊
Carbohydrate Polymers
Carbohydrate Polymers 化学-高分子科学
CiteScore
22.40
自引率
8.00%
发文量
1286
审稿时长
47 days
期刊介绍: Carbohydrate Polymers stands as a prominent journal in the glycoscience field, dedicated to exploring and harnessing the potential of polysaccharides with applications spanning bioenergy, bioplastics, biomaterials, biorefining, chemistry, drug delivery, food, health, nanotechnology, packaging, paper, pharmaceuticals, medicine, oil recovery, textiles, tissue engineering, wood, and various aspects of glycoscience. The journal emphasizes the central role of well-characterized carbohydrate polymers, highlighting their significance as the primary focus rather than a peripheral topic. Each paper must prominently feature at least one named carbohydrate polymer, evident in both citation and title, with a commitment to innovative research that advances scientific knowledge.
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