Carbonic anhydrase activity and metabolite variation of different microalgae species at alkaline pHs

Abstract

A significant increase in atmospheric greenhouse gases over the last century has led to the development of several methods and technologies to remove carbon dioxide (CO₂). Microorganisms produce carbonate minerals through the natural mineralization of CO₂; however, the feasibility of this process remains in research. This work aimed to study the cultivation of different microalgae under alkaline pH to maintain their potential for carbon mitigation. According to the results, the highest carbonic anhydrase activity has been reached (4.64 mg/g) for Chlamydomonas reinhardtii at a pH of 10. C. reinhardtii, at a pH of 9.5, yielded the highest chlorophyll content (23.58 mg/g), while Spirulina at a pH of 8.5 produced the highest biomass (882.9 mg/L). Also, a positive correlation existed between pH and lipid content for C. reinhardtii. Spirulina, however, exhibits the opposite effect. According to a principal component analysis, there is an opposite relationship between pH and carbonic anhydrase (CA) activity for C. reinhardtii and filamentous-type cyanobacteria from Salda Lake. The following order of the suitability of the microalgae species for high carbon capture is determined by the Analytic hierarchy process method: Spirulina> C. reinhardtii > Chlorella vulgaris > Coccus-type cyanobacteria > Filamentous-type cyanobacteria from Salda Lake. Additionally, this study provided important results regarding the cyanobacteria species isolated from an alkaline lake, Lake Salda. This would contribute to future studies of carbon capture and carbon mitigation mechanisms.