A ratiometric fluorescence platform based on bifunctional MOFs for sensitive detection of organophosphorus pesticides

IF 4 2区农林科学 Q2 CHEMISTRY, APPLIED Journal of Food Composition and Analysis Pub Date : 2024-11-13 DOI:10.1016/j.jfca.2024.106971

Siying Wu , Xuehua Zhang , Wenyan Tang , Yue Zhang , Xin Lv , Yu-Ting Zhuang

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Abstract

Rapid and precise detection of organophosphorus pesticides (OPs) is of great importance for life health. Herein, a new sensing platform is developed through a dual signal amplification approach for sensitive quantification of OPs, with a bimetallic metal organic framework (UiO-66(Zr/Fe)-NH₂) as peroxide-like nanozyme and fluorescent indicator. In the presence of thiocholine (TCh), the hydrolyzate of acetylthiocholine iodide catalyzed by acetylcholinesterase, the catalytic oxidization of o-phenylenediamine by UiO-66(Fe/Zr)-NH₂ to strong fluorescent 2,3-diaminophenazine (λ_em 555 nm) is significantly inhibited. OPs-triggered acetylcholinesterase inactivation enables the renaturation of UiO-66(Fe/Zr)-NH₂. Interestingly, the introduction of 1,4-dioxane further boosts the amplification of fluorescence signals. By virtue of the recovered fluorescence at 555 nm and MOF fluorescence (λ_em 446 nm), the dual signal amplification-based ratiometric fluorescent platform is successfully employed for the selective recognition and sensitive detection of OPs. This work is expected to provide a facile and sensitive method for accurate OPs assay, and make a positive contribution to food safety.

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基于双功能 MOFs 的比率荧光平台，用于灵敏检测有机磷农药

快速、精确地检测有机磷农药（OPs）对生命健康至关重要。本文以双金属金属有机框架（UiO-66(Zr/Fe)-NH2）作为过氧化物类纳米酶和荧光指示剂，通过双重信号放大方法开发了一种新的传感平台，用于灵敏地定量检测 OPs。在有硫代胆碱（TCh）（乙酰胆碱酯酶催化的乙酰硫代碘化胆碱的水解产物）存在的情况下，UiO-66（Fe/Zr）-NH2 催化邻苯二胺氧化成强荧光 2,3-二氨基吩嗪（λem 555 nm）的过程会受到明显抑制。OPs 触发的乙酰胆碱酯酶失活使 UiO-66（Fe/Zr）-NH2 重新饱和。有趣的是，1,4-二氧六环的引入进一步促进了荧光信号的放大。通过在 555 纳米波长和 MOF 荧光（λem 446 纳米波长）上的荧光恢复，基于双信号放大的比率荧光平台被成功地用于 OPs 的选择性识别和灵敏检测。这项工作有望为准确检测 OPs 提供一种简便灵敏的方法，为食品安全做出积极贡献。

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来源期刊

Journal of Food Composition and Analysis 工程技术-食品科技

CiteScore

6.20

自引率

11.60%

发文量

601

审稿时长

53 days

期刊介绍： The Journal of Food Composition and Analysis publishes manuscripts on scientific aspects of data on the chemical composition of human foods, with particular emphasis on actual data on composition of foods; analytical methods; studies on the manipulation, storage, distribution and use of food composition data; and studies on the statistics, use and distribution of such data and data systems. The Journal''s basis is nutrient composition, with increasing emphasis on bioactive non-nutrient and anti-nutrient components. Papers must provide sufficient description of the food samples, analytical methods, quality control procedures and statistical treatments of the data to permit the end users of the food composition data to evaluate the appropriateness of such data in their projects. The Journal does not publish papers on: microbiological compounds; sensory quality; aromatics/volatiles in food and wine; essential oils; organoleptic characteristics of food; physical properties; or clinical papers and pharmacology-related papers.