The PpWRKY22-PpWRKY70 regulatory module enhances resistance to Monilinia fructicola by regulating the gamma-aminobutyric acid shunt in peach fruit

IF 6.4 1区农林科学 Q1 AGRONOMY Postharvest Biology and Technology Pub Date : 2024-11-08 DOI:10.1016/j.postharvbio.2024.113306

Wenhui Li, Mei Dai, Xiaorui Wang, Yingying Shi, Zhimin Wang, Dapeng Li, Jingying Shi, Zunyang Song

{"title":"The PpWRKY22-PpWRKY70 regulatory module enhances resistance to Monilinia fructicola by regulating the gamma-aminobutyric acid shunt in peach fruit","authors":"Wenhui Li, Mei Dai, Xiaorui Wang, Yingying Shi, Zhimin Wang, Dapeng Li, Jingying Shi, Zunyang Song","doi":"10.1016/j.postharvbio.2024.113306","DOIUrl":null,"url":null,"abstract":"<div><div>Peach fruit is susceptible to infection of Monilinia fructicola leading to brown rot. Our previous study demonstrated that nitric oxide (NO) enhanced fruit resistance to M. fructicola by mediating the gamma-aminobutyric acid (GABA) shunt. Herein, two genes, PpWRKY22 and PpWRKY70, were identified whose expression was closely associated with the resistance of peach fruit against M. fructicola and induced by NO treatment. Notably, PpWRKY22 and PpWRKY70 activate the transcription of three GABA shunt-related genes (PpSSADH, PpGABA-T and PpGAD4) by directly binding to their promoters. Protein interaction assays revealed that PpWRKY22 interacts with PpWRKY70, and their protein complex enhances the promoter activities of PpSSADH, PpGABA-T and PpGAD4. Additionally, transient overexpression of PpWRKY22 and PpWRKY70 in peach fruit increases resistance to M. fructicola via up-regulating the transcription level of PpSSADH, PpGABA-T and PpGAD4. Overall, this study uncovers a novel model in which the interaction between PpWRKYs proteins actively enhances peach fruit resistance to M. fructicola by promoting the expression of GABA shunt-related genes.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"220 ","pages":"Article 113306"},"PeriodicalIF":6.4000,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Postharvest Biology and Technology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0925521424005519","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}

引用次数: 0

Abstract

Peach fruit is susceptible to infection of Monilinia fructicola leading to brown rot. Our previous study demonstrated that nitric oxide (NO) enhanced fruit resistance to M. fructicola by mediating the gamma-aminobutyric acid (GABA) shunt. Herein, two genes, PpWRKY22 and PpWRKY70, were identified whose expression was closely associated with the resistance of peach fruit against M. fructicola and induced by NO treatment. Notably, PpWRKY22 and PpWRKY70 activate the transcription of three GABA shunt-related genes (PpSSADH, PpGABA-T and PpGAD4) by directly binding to their promoters. Protein interaction assays revealed that PpWRKY22 interacts with PpWRKY70, and their protein complex enhances the promoter activities of PpSSADH, PpGABA-T and PpGAD4. Additionally, transient overexpression of PpWRKY22 and PpWRKY70 in peach fruit increases resistance to M. fructicola via up-regulating the transcription level of PpSSADH, PpGABA-T and PpGAD4. Overall, this study uncovers a novel model in which the interaction between PpWRKYs proteins actively enhances peach fruit resistance to M. fructicola by promoting the expression of GABA shunt-related genes.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

PpWRKY22-PpWRKY70 调控模块通过调节桃果实中的γ-氨基丁酸分流来增强对果孢霉的抗性

桃子果实易受果锈病莫尼林菌（Monilinia fructicola）感染，导致褐腐病。我们之前的研究表明，一氧化氮（NO）通过介导γ-氨基丁酸（GABA）分流增强了果实对果孢霉的抗性。在此，我们发现了两个基因 PpWRKY22 和 PpWRKY70，它们的表达与桃果对果蝇疫霉的抗性密切相关，并受 NO 处理的诱导。值得注意的是，PpWRKY22 和 PpWRKY70 通过直接与三个 GABA shunt 相关基因（PpSSADH、PpGABA-T 和 PpGAD4）的启动子结合，激活了这些基因的转录。蛋白质相互作用测定显示，PpWRKY22 与 PpWRKY70 相互作用，它们的蛋白质复合物增强了 PpSSADH、PpGABA-T 和 PpGAD4 的启动子活性。此外，通过上调 PpSSADH、PpGABA-T 和 PpGAD4 的转录水平，PpWRKY22 和 PpWRKY70 在桃果实中的瞬时过表达可增强对果孢霉的抗性。总之，本研究发现了一种新的模式，即 PpWRKYs 蛋白之间的相互作用通过促进 GABA shunt 相关基因的表达，积极增强了桃果实对果孢霉的抗性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Postharvest Biology and Technology 农林科学-农艺学

CiteScore

12.00

自引率

11.40%

发文量

309

审稿时长

38 days

期刊介绍： The journal is devoted exclusively to the publication of original papers, review articles and frontiers articles on biological and technological postharvest research. This includes the areas of postharvest storage, treatments and underpinning mechanisms, quality evaluation, packaging, handling and distribution of fresh horticultural crops including fruit, vegetables, flowers and nuts, but excluding grains, seeds and forages. Papers reporting novel insights from fundamental and interdisciplinary research will be particularly encouraged. These disciplines include systems biology, bioinformatics, entomology, plant physiology, plant pathology, (bio)chemistry, engineering, modelling, and technologies for nondestructive testing. Manuscripts on fresh food crops that will be further processed after postharvest storage, or on food processes beyond refrigeration, packaging and minimal processing will not be considered.