Regulation of lignin biosynthesis by GhCAD37 affects fiber quality and anther vitality in upland cotton.

IF 6.2 1区生物学 Q1 PLANT SCIENCES The Plant Journal Pub Date : 2024-11-19 DOI:10.1111/tpj.17149

Haipeng Li, Jinggong Guo, Kun Li, Yuwen Gao, Hang Li, Lu Long, Zongyan Chu, Yubei Du, Xulong Zhao, Bing Zhao, Chen Lan, José Ramón Botella, Xuebin Zhang, Kun-Peng Jia, Yuchen Miao

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Abstract

Cotton stands as a pillar in the textile industry due to its superior natural fibers. Lignin, a complex polymer synthesized from phenylalanine and deposited in mature cotton fibers, is believed to be essential for fiber quality, although the precise effects remain largely unclear. In this study, we characterized two ubiquitously expressed cinnamyl alcohol dehydrogenases (CAD), GhCAD37A and GhCAD37D (GhCAD37A/D), in Gossypium hirsutum. GhCAD37A/D possess CAD enzymatic activities, to catalyze the generation of monolignol products during lignin biosynthesis. Analysis of transgenic cotton knockout and overexpressing plants revealed that GhCAD37A/D are important regulators of fiber quality, positively impacting breaking strength but negatively affecting fiber length and elongation percentage by modulating lignin biosynthesis in fiber cells. Moreover, GhCAD37A/D are shown to modulate anther vitality and affect stem lodging trait in cotton by influencing lignin biosynthesis in the vascular bundles of anther and stem, respectively. Additionally, our study revealed that Ghcad37A/D knockout plants displayed red stem xylem, likely due to the overaccumulation of aldehyde intermediates in the phenylpropanoid metabolism pathway, as indicated by metabolomics analysis. Thus, our work illustrates that GhCAD37A/D are two important enzymes of lignin biosynthesis in different cotton organs, influencing fiber quality, anther vitality, and stem lodging.

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GhCAD37 对木质素生物合成的调控影响陆地棉的纤维质量和花药活力。

棉花因其优良的天然纤维而成为纺织业的支柱。木质素是一种由苯丙氨酸合成的复杂聚合物，沉积在成熟的棉纤维中，被认为对纤维质量至关重要，但其确切的影响在很大程度上仍不清楚。在这项研究中，我们鉴定了两种在棉花中普遍表达的肉桂醇脱氢酶（CAD），即 GhCAD37A 和 GhCAD37D（GhCAD37A/D）。GhCAD37A/D 具有 CAD 酶活性，在木质素生物合成过程中催化单木质素产物的生成。对转基因棉花基因敲除和过表达植株的分析表明，GhCAD37A/D 是纤维质量的重要调节因子，通过调节纤维细胞中木质素的生物合成，对纤维的断裂强度产生积极影响，但对纤维长度和伸长率产生消极影响。此外，GhCAD37A/D 还分别通过影响花药和茎维管束中木质素的生物合成来调节花药活力和影响棉花的茎结实性状。此外，我们的研究还发现，Ghcad37A/D 基因敲除植株的茎木质部呈红色，这可能是由于代谢组学分析表明，苯丙醇代谢途径中的醛中间产物过度积累所致。因此，我们的工作表明，GhCAD37A/D 是棉花不同器官中木质素生物合成的两个重要酶，影响着纤维质量、花药活力和茎秆宿存。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

The Plant Journal 生物-植物科学

CiteScore

13.10

自引率

4.20%

发文量

415

审稿时长

2.3 months

期刊介绍： Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community. Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.