Relative protective activities of avenanthramide A, B, and C against H2O2-induced endothelial dysfunction in EA.hy926 cells.

IF 1.4 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-11-18 DOI:10.1093/bbb/zbae170

Seungjoo Baik, Seonghwa Hong, Hyun Joo Kim, Heon Sang Jeong, Hana Lee, Junsoo Lee

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Abstract

This study compared the antihypertensive effects of avenanthramides A, B, and C, with a focus on their antioxidant and anti-inflammatory properties. Treatment with avenanthramides A, B, and C (50 μM) significantly enhanced cell viability and nitric oxide production in H2O2-induced endothelial dysfunction in EA.hy926 cells. Avenanthramides notably increased the levels of antioxidant enzymes and glutathione while reducing malondialdehyde and reactive oxygen species. Moreover, avenanthramides promoted the Nrf2 translocation to nucleus, enhancing the expression of antioxidant enzymes. Furthermore, avenanthramides inhibited the protein levels of iNOS and COX-2, as well as the phosphorylation of IkBα and translocation of p65, thereby mitigating endothelial inflammation. Molecular docking analysis revealed that avenanthramide A exhibited the strongest binding affinity for HO-1 and iNOS, which was correlated with its superior biological activity. Overall, by upregulating Nrf2/HO-1 pathways and downregulating NF-kB pathways, avenanthramides show potential as therapeutic agents for the treatment of endothelial dysfunction.

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文竹酰胺 A、B 和 C 对 EA.hy926 细胞中 H2O2 诱导的内皮功能障碍的相对保护活性。

本研究比较了芒柄菊甙A、B和C的抗高血压作用，重点研究了它们的抗氧化和抗炎特性。在H2O2诱导的EA.hy926细胞内皮功能障碍中，用枳椇子A、B和C（50 μM）处理可显著提高细胞活力和一氧化氮的产生。蛇床子甙明显提高了抗氧化酶和谷胱甘肽的水平，同时减少了丙二醛和活性氧。此外，蛇苋酰胺还能促进Nrf2向细胞核转位，增强抗氧化酶的表达。此外，车前子甙还能抑制 iNOS 和 COX-2 蛋白水平，以及 IkBα 的磷酸化和 p65 的转位，从而缓解内皮炎症。分子对接分析表明，venanthramide A与HO-1和iNOS的结合亲和力最强，这与其卓越的生物活性相关。总之，通过上调Nrf2/HO-1通路和下调NF-kB通路，venanthramides显示出作为治疗内皮功能障碍的药物的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioscience, Biotechnology, and Biochemistry 生物-生化与分子生物学

CiteScore

3.50

自引率

0.00%

发文量

183

审稿时长

1 months

期刊介绍： Bioscience, Biotechnology, and Biochemistry publishes high-quality papers providing chemical and biological analyses of vital phenomena exhibited by animals, plants, and microorganisms, the chemical structures and functions of their products, and related matters. The Journal plays a major role in communicating to a global audience outstanding basic and applied research in all fields subsumed by the Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA).