Novel Rat Model of Embolic Cerebral Ischemia Using a Radiopaque Blood Clot and a Microcatheter Under Fluoroscopy.

IF 3.8 2区医学 Q1 CLINICAL NEUROLOGY Translational Stroke Research Pub Date : 2024-11-19 DOI:10.1007/s12975-024-01312-2

Teppei Komatsu, Hiroki Ohta, Misato Takeda, Yasuhiro Matsumura, Masayuki Yokoyama, Zuojun Wang, Hirotaka James Okano, Yasuyuki Iguchi

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引用次数: 0

Abstract

Conventional rat models of thromboembolic stroke do not allow control of infarct size or spontaneous recanalization. We aimed to develop a novel rat thromboembolic stroke model that ensures highly reproducible infarct sizes and locations within the MCA territory and does not require arterial ligation. Twenty male Sprague-Dawley rats and two sham-operated rats were included. A microcatheter was navigated from the caudal ventral artery to the internal carotid artery using digital subtraction angiography. A blood clot (diameter, 0.86 mm; length, 3 mm) containing zirconium dioxide was advanced in the catheter. Fluoroscopy was performed at 1, 3, 6, and 24 h after stroke model creation, and TTC staining was conducted at 24 h. Neurological deficit scores were measured. In all embolized rats, the ACA and MCA bifurcation were selective. Median operating time was 6 min. The position of the radiopaque blood clot remained unchanged for 24 h after model creation in 19/20 rats. Median infarct volume was 242 mm³ (IQR, 239-262 mm³). We present a novel rat model of highly reproducible focal infarct in only the MCA territory. Fluoroscopy effectively identified any blood clot migration. This model could contribute to the development of new thrombolytic agents.

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在透视下使用不透射线血凝块和微导管建立栓塞性脑缺血的新型大鼠模型

传统的血栓栓塞性中风大鼠模型无法控制梗塞大小或自发再通畅。我们旨在开发一种新型大鼠血栓栓塞性中风模型，它能确保 MCA 区域内梗塞大小和位置的高度再现性，并且不需要动脉结扎。研究对象包括 20 只雄性 Sprague-Dawley 大鼠和两只假手术大鼠。使用数字减影血管造影术将微导管从尾部腹侧动脉导航到颈内动脉。将含有二氧化锆的血凝块（直径 0.86 毫米；长度 3 毫米）推进导管。中风模型建立后 1、3、6 和 24 小时进行透视检查，24 小时进行 TTC 染色。所有栓塞的大鼠都选择了 ACA 和 MCA 分叉。中位手术时间为 6 分钟。在 19/20 只大鼠中，不透射线血凝块的位置在模型创建后 24 小时内保持不变。梗死体积中位数为 242 mm3（IQR，239-262 mm3）。我们展示了一种仅在 MCA 区域发生高度可重现的局灶性脑梗塞的新型大鼠模型。透视检查可有效识别血凝块迁移。该模型有助于开发新的溶栓药物。

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来源期刊

Translational Stroke Research CLINICAL NEUROLOGY-NEUROSCIENCES

CiteScore

13.80

自引率

4.30%

发文量

130

审稿时长

6-12 weeks

期刊介绍： Translational Stroke Research covers basic, translational, and clinical studies. The Journal emphasizes novel approaches to help both to understand clinical phenomenon through basic science tools, and to translate basic science discoveries into the development of new strategies for the prevention, assessment, treatment, and enhancement of central nervous system repair after stroke and other forms of neurotrauma. Translational Stroke Research focuses on translational research and is relevant to both basic scientists and physicians, including but not restricted to neuroscientists, vascular biologists, neurologists, neuroimagers, and neurosurgeons.