Aloperine Attenuates UVB-induced Damage in Skin Fibroblasts Via Activating TFE3/Beclin-1-Mediated Autophagy.

IF 1 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Protein and Peptide Letters Pub Date : 2024-11-15 DOI:10.2174/0109298665335370241017055831

Mingning Qiu, Jianchang Li, Shuai Zhang, Jinglan Liang, Xuguang Wang, Jie Liu

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Abstract

Background: Aloperine (ALO) is an important active ingredient in the traditional Chinese medicinal plant Sophora alopecuroides L and has a significant autophagy-stimulating effect. The effect of ALO on cytotoxicity caused by UVB radiation in skin fibroblasts and the potential mechanism remains unclear.

Objective: The present study aimed to assess the effect of ALO on UVB-induced damage in skin fibroblasts and investigate its possible mechanism.

Methods: Cell viability, cytotoxicity, caspase-Glo 3/7 activity, apoptosis, and protein expression were measured in UVB-treated skin fibroblasts in the presence or absence of ALO. Autophagy inhibitors (chloroquine and bafilomycin A1) and TFE3 siRNA transfection were used to elucidate the potential mechanisms further.

Results: These data demonstrate that ALO attenuated cell viability inhibition, apoptosis, cytotoxicity, and alterations in autophagy-related proteins caused by UVB exposure in skin fibroblasts. ALO stimulates autophagy activation and TFE3 nuclear localization in UVB-treated skin fibroblasts. Furthermore, treatment with autophagy inhibitors and TFE3 siRNA reversed the effects of ALO on UVB-treated skin fibroblasts.

Conclusion: These results suggest that ALO protects skin fibroblasts against UVB-induced cytotoxicity by stimulating TFE3/Beclin-1-mediated autophagy.

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阿洛哌啶通过激活 TFE3/Beclin-1 介导的自噬减轻紫外线诱导的皮肤成纤维细胞损伤

背景：阿洛哌啶（ALO）是传统中药植物槐树中的一种重要活性成分，具有显著的自噬刺激作用。ALO 对紫外线辐射引起的皮肤成纤维细胞毒性的影响及其潜在机制尚不清楚：本研究旨在评估 ALO 对 UVB 诱导的皮肤成纤维细胞损伤的影响，并探讨其可能的机制：方法：在ALO存在或不存在的情况下，测量经UVB处理的皮肤成纤维细胞的细胞活力、细胞毒性、Caspase-Glo 3/7活性、细胞凋亡和蛋白质表达。自噬抑制剂（氯喹和巴佛洛霉素 A1）和 TFE3 siRNA 转染被用来进一步阐明潜在的机制：这些数据表明，ALO 可减轻 UVB 暴露对皮肤成纤维细胞造成的细胞活力抑制、细胞凋亡、细胞毒性和自噬相关蛋白的改变。ALO 可刺激经 UVB 处理的皮肤成纤维细胞的自噬激活和 TFE3 核定位。此外，用自噬抑制剂和TFE3 siRNA处理可逆转ALO对经UVB处理的皮肤成纤维细胞的影响：这些结果表明，ALO通过刺激TFE3/Beclin-1介导的自噬，保护皮肤成纤维细胞免受UVB诱导的细胞毒性的伤害。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Protein and Peptide Letters 生物-生化与分子生物学

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

2 months

期刊介绍： Protein & Peptide Letters publishes letters, original research papers, mini-reviews and guest edited issues in all important aspects of protein and peptide research, including structural studies, advances in recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, and drug design. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallization and preliminary structure determination of biologically important proteins are considered only if they include significant new approaches or deal with proteins of immediate importance, and preliminary structure determinations of biologically important proteins. Purely theoretical/review papers should provide new insight into the principles of protein/peptide structure and function. Manuscripts describing computational work should include some experimental data to provide confirmation of the results of calculations. Protein & Peptide Letters focuses on: Structure Studies Advances in Recombinant Expression Drug Design Chemical Synthesis Function Pharmacology Enzymology Conformational Analysis Immunology Biotechnology Protein Engineering Protein Folding Sequencing Molecular Recognition Purification and Analysis