Effects of hyperhomocysteinemia on follicular development and oocytes quality.

IF 4.6 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES iScience Pub Date : 2024-10-23 eCollection Date: 2024-11-15 DOI:10.1016/j.isci.2024.111241

Lu Wang, Jinmei Gao, Jie Ma, Jing Sun, Yajie Wang, Jia Luo, Zhaoyang Wang, Hui Wang, Jialing Li, Danyu Yang, Jinfang Wang, Rong Hu

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Abstract

In patients with polycystic ovary syndrome (PCOS), the concentration of homocysteine (Hcy) in follicular fluid is inversely correlated with oocyte and embryo quality. Nevertheless, other metabolic abnormalities associated with PCOS may also impact oocyte and early embryo quality. Therefore, it remains uncertain whether reproductive function is affected in patients without PCOS with hyperhomocysteinemia (HHcy). Here, we observed reduced fertility, increased ovarian atretic follicles, and reduced oocyte maturation rates in HHcy mice. Proteomic analyses revealed that HHcy causes mitochondrial dysfunction and reduced expression of zona pellucida proteins (ZP1, ZP2, and ZP3) in oocytes. Transmission electron microscopy confirmed abnormal formation of the zona pellucida and microvilli in oocytes from HHcy mice. Additionally, in vitro fertilization (IVF) demonstrated a reduction in the rate of 2-cell embryo formation in HHcy mice. These findings reveal that HHcy reduces female reproductive longevity by affecting follicular development and oocyte quality.

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高同型半胱氨酸血症对卵泡发育和卵母细胞质量的影响

在多囊卵巢综合征（PCOS）患者中，卵泡液中的同型半胱氨酸（Hcy）浓度与卵母细胞和胚胎质量成反比。然而，与多囊卵巢综合征相关的其他代谢异常也可能影响卵母细胞和早期胚胎的质量。因此，目前仍不确定无多囊卵巢综合症的高同型半胱氨酸血症（HHcy）患者的生殖功能是否会受到影响。在这里，我们观察到 HHcy 小鼠的生育能力下降、卵巢闭锁卵泡增加、卵母细胞成熟率降低。蛋白质组分析表明，HHcy 会导致线粒体功能障碍以及卵母细胞中透明带蛋白（ZP1、ZP2 和 ZP3）表达减少。透射电子显微镜证实，HHcy 小鼠卵母细胞中透明带和微绒毛的形成异常。此外，体外受精（IVF）表明，HHcy 小鼠的 2 细胞胚胎形成率降低。这些研究结果表明，HHcy 会影响卵泡发育和卵母细胞质量，从而降低雌性生殖寿命。

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来源期刊

iScience Multidisciplinary-Multidisciplinary

CiteScore

7.20

自引率

1.70%

发文量

1972

审稿时长

6 weeks

期刊介绍： Science has many big remaining questions. To address them, we will need to work collaboratively and across disciplines. The goal of iScience is to help fuel that type of interdisciplinary thinking. iScience is a new open-access journal from Cell Press that provides a platform for original research in the life, physical, and earth sciences. The primary criterion for publication in iScience is a significant contribution to a relevant field combined with robust results and underlying methodology. The advances appearing in iScience include both fundamental and applied investigations across this interdisciplinary range of topic areas. To support transparency in scientific investigation, we are happy to consider replication studies and papers that describe negative results. We know you want your work to be published quickly and to be widely visible within your community and beyond. With the strong international reputation of Cell Press behind it, publication in iScience will help your work garner the attention and recognition it merits. Like all Cell Press journals, iScience prioritizes rapid publication. Our editorial team pays special attention to high-quality author service and to efficient, clear-cut decisions based on the information available within the manuscript. iScience taps into the expertise across Cell Press journals and selected partners to inform our editorial decisions and help publish your science in a timely and seamless way.