Protein profile of mouse endolymph suggests a role in controlling cochlear homeostasis.

IF 4.6 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES iScience Pub Date : 2024-10-19 eCollection Date: 2024-11-15 DOI:10.1016/j.isci.2024.111214

Masatoshi Fukuda, Hiroki Okanishi, Daisuke Ino, Kazuya Ono, Takeru Ota, Eri Wakai, Takashi Sato, Yumi Ohta, Yoshiaki Kikkawa, Hidenori Inohara, Yoshikatsu Kanai, Hiroshi Hibino

{"title":"Protein profile of mouse endolymph suggests a role in controlling cochlear homeostasis.","authors":"Masatoshi Fukuda, Hiroki Okanishi, Daisuke Ino, Kazuya Ono, Takeru Ota, Eri Wakai, Takashi Sato, Yumi Ohta, Yoshiaki Kikkawa, Hidenori Inohara, Yoshikatsu Kanai, Hiroshi Hibino","doi":"10.1016/j.isci.2024.111214","DOIUrl":null,"url":null,"abstract":"The cochlea contains two extracellular fluids, perilymph and endolymph. Endolymph exhibits high potential of approximately +80 to +110 mV (depending on species), which sensitizes sensory hair cells. Other properties of this unique fluid remain elusive, owing to its minuscule volume in rodent cochlea. We therefore developed a technique to collect high-purity endolymph from mouse cochleae. Comprehensive proteomic analysis of sampled endolymph using liquid chromatography with mass spectrometry identified 301 proteins, dominated by molecules engaged in immunity and proteostasis. Approximately 30% of these proteins were undetectable in our perilymph. A combination of mass spectrometry and different approaches revealed that, compared to perilymph, endolymph was enriched with α2-macroglobulin, osteopontin, apolipoprotein D, apolipoprotein E, and apolipoprotein J/clusterin. In other cells or tissues, α2-macroglobulin, apolipoprotein E, and apolipoprotein J contribute to the clearance of degraded proteins from extracellular fluid. Altogether, with the proteins described here, endolymph may play a protective role in stabilizing cochlear homeostasis.","PeriodicalId":342,"journal":{"name":"iScience","volume":"27 11","pages":"111214"},"PeriodicalIF":4.6000,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11574807/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"iScience","FirstCategoryId":"103","ListUrlMain":"https://doi.org/10.1016/j.isci.2024.111214","RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/11/15 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

The cochlea contains two extracellular fluids, perilymph and endolymph. Endolymph exhibits high potential of approximately +80 to +110 mV (depending on species), which sensitizes sensory hair cells. Other properties of this unique fluid remain elusive, owing to its minuscule volume in rodent cochlea. We therefore developed a technique to collect high-purity endolymph from mouse cochleae. Comprehensive proteomic analysis of sampled endolymph using liquid chromatography with mass spectrometry identified 301 proteins, dominated by molecules engaged in immunity and proteostasis. Approximately 30% of these proteins were undetectable in our perilymph. A combination of mass spectrometry and different approaches revealed that, compared to perilymph, endolymph was enriched with α₂-macroglobulin, osteopontin, apolipoprotein D, apolipoprotein E, and apolipoprotein J/clusterin. In other cells or tissues, α₂-macroglobulin, apolipoprotein E, and apolipoprotein J contribute to the clearance of degraded proteins from extracellular fluid. Altogether, with the proteins described here, endolymph may play a protective role in stabilizing cochlear homeostasis.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

小鼠内淋巴的蛋白质特征表明其在控制耳蜗稳态中的作用。

耳蜗内有两种细胞外液，即耳周液和内淋巴液。内淋巴具有约 +80 至 +110 mV 的高电位（视物种而定），能敏化感觉毛细胞。由于内淋巴在啮齿动物耳蜗中的体积极小，这种独特液体的其他特性仍然难以捉摸。因此，我们开发了一种从小鼠耳蜗中收集高纯度内淋巴的技术。利用液相色谱-质谱法对取样的内淋巴进行了全面的蛋白质组分析，发现了301种蛋白质，其中主要是参与免疫和蛋白稳态的分子。其中约 30% 的蛋白质在我们的淋巴结中检测不到。质谱分析和不同方法的结合显示，与耳周液相比，内淋巴中富含α2-巨球蛋白、补骨脂素、载脂蛋白D、载脂蛋白E和载脂蛋白J/clusterin。在其他细胞或组织中，α2-巨球蛋白、脂蛋白 E 和脂蛋白 J 有助于清除细胞外液中的降解蛋白质。综上所述，内淋巴中的这些蛋白质可在稳定耳蜗平衡方面发挥保护作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

iScience Multidisciplinary-Multidisciplinary

CiteScore

7.20

自引率

1.70%

发文量

1972

审稿时长

6 weeks

期刊介绍： Science has many big remaining questions. To address them, we will need to work collaboratively and across disciplines. The goal of iScience is to help fuel that type of interdisciplinary thinking. iScience is a new open-access journal from Cell Press that provides a platform for original research in the life, physical, and earth sciences. The primary criterion for publication in iScience is a significant contribution to a relevant field combined with robust results and underlying methodology. The advances appearing in iScience include both fundamental and applied investigations across this interdisciplinary range of topic areas. To support transparency in scientific investigation, we are happy to consider replication studies and papers that describe negative results. We know you want your work to be published quickly and to be widely visible within your community and beyond. With the strong international reputation of Cell Press behind it, publication in iScience will help your work garner the attention and recognition it merits. Like all Cell Press journals, iScience prioritizes rapid publication. Our editorial team pays special attention to high-quality author service and to efficient, clear-cut decisions based on the information available within the manuscript. iScience taps into the expertise across Cell Press journals and selected partners to inform our editorial decisions and help publish your science in a timely and seamless way.