Enantioselective electrochemical detection of a L-and D-serine at polyvinylpyrrolidone-modified platinum electrode.

IF 1.9 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS Bioanalysis Pub Date : 2024-11-20 DOI:10.1080/17576180.2024.2422209

Mohammad Amayreh, Mohammed Khair Hourani

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Abstract

Aim: By modifying the Pt electrode with polyvinylpyrrolidone, the distinct oxidation potentials of L and D-serine were markedly increased for both isomers.Methods & results: CV was used for Pt electrode modification with PVP. Twenty cycles was reasonable for Pt modification. K₃[Fe(CN)₆] CV experiments investigated reversibility of the Fe(III) redox reaction and the enhancement of electron transfer at the PVP-Pt electrode. L and D-serine showing an oxidation peak potential at 0.394 V and 0.468 V, respectively. A linear relationship between the anodic oxidation current extracted from the CVs and the standard concentrations of L-serine and D-serine solutions was obtained with R² = 0.99. The dynamic range for D-serine was from 0.5 to 20 mM with a LOD of 0.16 mM, while for L-serine, was from 2.5 to 20 mM with a detection limit of 0.27 mM. Using DPV, the dynamic range was 0.05-1.0 µM for D-serine with a detection limit of 0.0103 µM. The standard deviation ranged from 0.212 to 0.38 across ten determinations per concentration.Conclusion: A separation by 74 mV between the oxidation peaks of L and D-serine was achieved with remarkable enhancement in oxidation current for both isomers. PVP-Pt electrode can detect D-serine in the presence of DL-serine.

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在聚乙烯吡咯烷酮修饰的铂电极上对 L 型和 D 型丝氨酸进行对映选择性电化学检测。

目的：通过用聚乙烯吡咯烷酮修饰铂电极，L-丝氨酸和 D-丝氨酸两种异构体的不同氧化电位明显提高：用聚乙烯吡咯烷酮修饰铂电极时采用了 CV 法。铂修饰的合理循环次数为 20 次。K3[Fe(CN)6] CV 实验研究了铁(III)氧化还原反应的可逆性以及 PVP-Pt 电极上电子转移的增强。L 和 D-丝氨酸的氧化峰电位分别为 0.394 V 和 0.468 V。从 CVs 中提取的阳极氧化电流与 L-丝氨酸和 D-丝氨酸溶液的标准浓度之间呈线性关系，R2 = 0.99。D -丝氨酸的动态范围为 0.5 至 20 毫摩尔，检测限为 0.16 毫摩尔；L -丝氨酸的动态范围为 2.5 至 20 毫摩尔，检测限为 0.27 毫摩尔。使用 DPV，D-丝氨酸的动态范围为 0.05-1.0 µM，检测限为 0.0103 µM。在每个浓度的十次测定中，标准偏差在 0.212 至 0.38 之间：结论：L-丝氨酸和 D-丝氨酸的氧化峰之间的电压相差 74 mV，两种异构体的氧化电流都显著增加。PVP-Pt 电极可在 DL-丝氨酸存在的情况下检测 D-丝氨酸。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioanalysis BIOCHEMICAL RESEARCH METHODS-CHEMISTRY, ANALYTICAL

CiteScore

3.30

自引率

16.70%

发文量

审稿时长

2 months

期刊介绍： Reliable data obtained from selective, sensitive and reproducible analysis of xenobiotics and biotics in biological samples is a fundamental and crucial part of every successful drug development program. The same principles can also apply to many other areas of research such as forensic science, toxicology and sports doping testing. The bioanalytical field incorporates sophisticated techniques linking sample preparation and advanced separations with MS and NMR detection systems, automation and robotics. Standards set by regulatory bodies regarding method development and validation increasingly define the boundaries between speed and quality. Bioanalysis is a progressive discipline for which the future holds many exciting opportunities to further reduce sample volumes, analysis cost and environmental impact, as well as to improve sensitivity, specificity, accuracy, efficiency, assay throughput, data quality, data handling and processing. The journal Bioanalysis focuses on the techniques and methods used for the detection or quantitative study of analytes in human or animal biological samples. Bioanalysis encourages the submission of articles describing forward-looking applications, including biosensors, microfluidics, miniaturized analytical devices, and new hyphenated and multi-dimensional techniques. Bioanalysis delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for the modern bioanalyst.