An Observational Case-control Study for BDNF Val66Met Polymorphism and Serum BDNF in Patients with Major Depressive Disorder (MDD).

IF 1.9 Q3 PSYCHIATRY Indian Journal of Psychological Medicine Pub Date : 2024-09-25 DOI:10.1177/02537176241280050

Raja Babu Ramawat, Rizwana Quraishi, Raman Deep, Ram Kumar, Ashwani Kumar Mishra, Raka Jain

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Abstract

Background: The brain-derived neurotrophic factor (BDNF) has an important role in the growth of neurons and synaptic transmission. The BDNF gene Val66Met polymorphism (G/A) is associated with depression, but findings have not been consistently replicated. This study adopts a case-control design with an aim to investigate the association of Val66Met polymorphism and peripheral BDNF (serum) levels in patients of major depressive disorder (MDD) and healthy individuals (controls).

Materials and method: This study adopts an observational, case-control design with a total of 174 participants. Cases (n = 87) were currently depressed, having Diagnostic and Statistical Manual of Mental Disorders (DSM-5) MDD, without psychiatric comorbidity. Controls (n = 87) comprised healthy individuals with no family history of psychiatric illness. The cases were evaluated using the NIMH-Life Chart Method, Hamilton Depression Rating Scale (HAM-D), Clinically Useful Depression Outcome Scale (CUDOS), and Clinical Global Impression (CGI). TaqMan assay was used for genotyping, and serum BDNF was measured using Enzyme-linked immunosorbent assay (ELISA).

Results: The case mean age was 35.32 ± 11.69 years (52% females) and comparable to controls. Allelic distribution was 33% (Met), and genotypic distribution was 17% (Met/Met), 32% (Val/Met), and 51% (Val/Val) for cases. The genotypic distribution did not differ across study groups. Serum BDNF was significantly lower in MDD cases as compared to controls (p < .001). The serum BDNF levels were comparable across the genotypic groups among cases.

Conclusion: The Val66Met polymorphism has not been associated with a risk for MDD and, interestingly, did not influence the BDNF levels (serum). Significantly low BDNF levels were found in MDD cases. The study findings show that factors other than Val66Met gene polymorphism have a role in modulating serum BDNF levels.

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重度抑郁障碍 (MDD) 患者 BDNF Val66Met 多态性与血清 BDNF 的病例对照观察研究。

背景：脑源性神经营养因子（BDNF脑源性神经营养因子（BDNF）在神经元生长和突触传递中发挥着重要作用。BDNF基因Val66Met多态性（G/A）与抑郁症有关，但研究结果尚未得到一致证实。本研究采用病例对照设计，旨在调查重度抑郁症（MDD）患者和健康人（对照组）的 Val66Met 多态性与外周 BDNF（血清）水平的相关性：本研究采用观察性病例对照设计，共有 174 名参与者。病例（n = 87）为目前患有《精神疾病诊断与统计手册》（DSM-5）MDD 的抑郁症患者，无精神疾病合并症。对照组（87 人）由无精神病家族史的健康人组成。采用 NIMH 生活图表法、汉密尔顿抑郁量表 (HAM-D)、临床实用抑郁结果量表 (CUDOS) 和临床整体印象 (CGI) 对病例进行评估。基因分型采用 TaqMan 法，血清 BDNF 采用酶联免疫吸附法（ELISA）：病例平均年龄为 35.32 ± 11.69 岁（女性占 52%），与对照组相当。病例的等位基因分布为 33%（Met），基因型分布为 17%（Met/Met）、32%（Val/Met）和 51%（Val/Val）。各研究组的基因型分布没有差异。与对照组相比，MDD 病例的血清 BDNF 水平明显较低（p .001）。不同基因型组病例的血清 BDNF 水平相当：结论：Val66Met 多态性与罹患 MDD 的风险无关，有趣的是，它不会影响 BDNF 水平（血清）。在 MDD 病例中，BDNF 水平明显偏低。研究结果表明，除 Val66Met 基因多态性外，其他因素也能调节血清 BDNF 水平。

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来源期刊

Indian Journal of Psychological Medicine PSYCHOLOGY-

CiteScore

4.80

自引率

7.10%

发文量

116

审稿时长

12 weeks

期刊介绍： The Indian Journal of Psychological Medicine (ISSN 0253-7176) was started in 1978 as the official publication of the Indian Psychiatric Society South Zonal Branch. The journal allows free access (Open Access) and is published Bimonthly. The Journal includes but is not limited to review articles, original research, opinions, and letters. The Editor and publisher accept no legal responsibility for any opinions, omissions or errors by the authors, nor do they approve of any product advertised within the journal.