Characterization of biogenic selenium nanoparticles in hypersaline media by single particle inductively coupled plasma mass spectrometry: Haloferax mediterranei case.

IF 5.7 2区 化学 Q1 CHEMISTRY, ANALYTICAL Analytica Chimica Acta Pub Date : 2024-11-21 DOI:10.1016/j.aca.2024.343453
Nuria Guijarro-Ramírez, Iraide Sáez-Zamacona, Daniel Torregrosa, Guillermo Grindlay, Luis Gras, Carmen Pire, Juan Mora, Rosa María Martínez-Espinosa
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Abstract

Background

Single particle inductively coupled plasma mass spectrometry (spICP-MS) is extensively employed for the characterization of biogenic selenium nanoparticles (SeNPs) produced by mesophilic microorganisms. Nevertheless, because halophilic microorganisms are also well-known to produce SeNPs, further research efforts are required to investigate spICP-MS applicability for characterizing such nanomaterials in hypersaline media. The goal of this work is to develop a methodology for characterizing SeNPs in hypersaline media by spICP-MS. To this end, plasma operating conditions, non-spectral interferences and calibration strategies were investigated. The proposed method was employed to investigate the capabilities of the halophilic archaea Haloferax mediterranei to produce SeNPs.

Results

By the appropriate selection of experimental conditions, SeNPs can be accurately analyzed in hypersaline media by spICP-MS. Unlike previous works in the literature, no differences in ionic signal were observed between SeNPs and dissolved Se and, hence, there is no need to apply any empirical corrector factor for obtaining accurate particle size distributions. Non-spectral interferences are mitigated by diluting the sample at least 1:103 which allows the use of water standards. Size (30 nm) and particle (7 x 105 particles mL-1) detection limits were low enough to characterize biogenic SeNPs produced by halophilic microorganisms. The use of the optimized methodology reveals that Haloferax mediterranei can produce SeNPs when it is exposed to selenite up to 1 mM, but no formation is produced for selenate exposure. Depending on incubation parameters (selenite concentration and time), the particle median diameter ranged from 80-100 nm, whereas particle concentration varied from 0.8 to 1.9 x1013 particles mL-1.

Significance

This represents the first methodology for characterizing biogenic SeNPs in hypersaline media by spICP-MS with accuracy and precision using non-matrix matched standards. It opens the opportunity to investigate the capabilities of halophilic microorganisms (e.g., H. mediterranei) to produce Se-based nanomaterials.

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单颗粒电感耦合等离子体质谱法表征高盐介质中的生物硒纳米颗粒:米德兰光蜡(Haloferax mediterranei)案例。
背景单颗粒电感耦合等离子体质谱法(spICP-MS)被广泛用于表征中嗜酸性微生物产生的生物硒纳米颗粒(SeNPs)。然而,由于众所周知嗜卤微生物也会产生 SeNPs,因此需要进一步研究 spICP-MS 是否适用于表征超盐介质中的此类纳米材料。这项工作的目标是开发一种利用 spICP-MS 表征超碱性介质中 SeNPs 的方法。为此,研究了等离子体操作条件、非光谱干扰和校准策略。结果 通过适当选择实验条件,spICP-MS 可以准确分析超碱性介质中的 SeNPs。与之前的文献不同,SeNPs 和溶解的 Se 之间没有观察到离子信号的差异,因此无需应用任何经验校正因子来获得准确的粒度分布。通过将样品稀释至少 1:103,可以使用水标准,从而减轻非光谱干扰。粒度(30 nm)和颗粒(7 x 105 个颗粒 mL-1)的检测限很低,足以确定嗜卤微生物产生的生物源 SeNPs 的特征。使用优化方法发现,当 Haloferax mediterranei 暴露于高达 1 mM 的亚硒酸盐时,它可以产生 SeNPs,但暴露于硒酸盐时则不会产生。根据培养参数(亚硒酸盐浓度和时间)的不同,颗粒的中值直径在 80-100 nm 之间,而颗粒浓度则在 0.8-1.9 x1013 颗粒 mL-1 之间变化。它为研究嗜卤微生物(如 H. mediterranei)生产 Se 基纳米材料的能力提供了机会。
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来源期刊
Analytica Chimica Acta
Analytica Chimica Acta 化学-分析化学
CiteScore
10.40
自引率
6.50%
发文量
1081
审稿时长
38 days
期刊介绍: Analytica Chimica Acta has an open access mirror journal Analytica Chimica Acta: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. Analytica Chimica Acta provides a forum for the rapid publication of original research, and critical, comprehensive reviews dealing with all aspects of fundamental and applied modern analytical chemistry. The journal welcomes the submission of research papers which report studies concerning the development of new and significant analytical methodologies. In determining the suitability of submitted articles for publication, particular scrutiny will be placed on the degree of novelty and impact of the research and the extent to which it adds to the existing body of knowledge in analytical chemistry.
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