Identification and expression analysis of the SPL gene family during flower bud differentiation in Rhododendron molle.

IF 1.6 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Genes & genomics Pub Date : 2024-11-20 DOI:10.1007/s13258-024-01593-9

Dongmei Zhu, Xingmin Geng, Fanyu Zeng, Shida Xu, Jieyu Peng

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Abstract

Background: The family of SQUAMOSA promoter binding protein-like (SPL) transcription factors is essential for regulating plant growth and development. While this SPL gene functional research has been limited in Rhododendron molle (R. molle).

Objective: To preliminarily explore the regulatory mechanism of the SPL gene in flower bud development of R. molle.

Methods: In this study, for R. molle, the flower bud differentiation period was determined by observing the morphological anatomy of the flower bud. The SPL gene family members were identified based on the R. molle genome, Additionally, the expressions of RmSPL genes at five flower bud differentiation stages were analyzed via Quantitative reverse transcription PCR (RT-qPCR).

Results: We first characterized 20 SPL family members in the reference genome of R. molle. The phylogenetic analysis of plant SPL proteins separated them into eight subfamilies (G1-G8) according to conserved gene structures and protein motifs. Cis-elements of promoter region analysis showed that RmSPL genes were regulated by light, phytohormones, stress response, and plant growth and development and may play a critical role in the photoresponse, abasic acid, anaerobic induction, and meristematic expression. Gene expression analysis showed that 18 RmSPL genes were differentially expressed in different developing flower buds. In particular, RmSPL1/7/8/12/13 exhibited significantly different expressions, suggesting that they were likely essential genes for regulating the differentiation of flower buds.

Conclusion: In conclusion, our analysis of RmSPL genes provides a theoretical basis and reference for future functional analysis of RmSPL genes in the flower bud differentiation of R. molle.

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杜鹃花花芽分化过程中 SPL 基因家族的鉴定和表达分析

背景：SQUAMOSA类启动子结合蛋白（SPL）转录因子家族是调控植物生长和发育的重要因子。而在杜鹃花（Rododendron molle）中，SPL 基因的功能研究还很有限：初步探讨 SPL 基因在杜鹃花花芽发育过程中的调控机制：方法：本研究通过观察花芽的形态解剖学特征，确定花芽分化期。此外，还通过定量反转录 PCR（RT-qPCR）分析了五个花芽分化期 RmSPL 基因的表达情况：结果：我们首先鉴定了R. molle参考基因组中20个SPL家族成员的特征。根据保守的基因结构和蛋白基序，植物 SPL 蛋白的系统进化分析将其分为八个亚家族（G1-G8）。启动子区域顺式元件分析表明，RmSPL基因受光照、植物激素、胁迫响应和植物生长发育的调控，可能在光响应、消旋体酸、厌氧诱导和分生组织表达中起关键作用。基因表达分析表明，18 个 RmSPL 基因在不同的发育花芽中存在差异表达。其中，RmSPL1/7/8/12/13表现出显著的表达差异，表明它们可能是调控花芽分化的重要基因：总之，我们对 RmSPL 基因的分析为今后对 RmSPL 基因在莫莱尔花芽分化中的功能分析提供了理论依据和参考。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Genes & genomics 生物-生化与分子生物学

CiteScore

3.70

自引率

4.80%

发文量

131

审稿时长

6-12 weeks

期刊介绍： Genes & Genomics is an official journal of the Korean Genetics Society (http://kgenetics.or.kr/). Although it is an official publication of the Genetics Society of Korea, membership of the Society is not required for contributors. It is a peer-reviewed international journal publishing print (ISSN 1976-9571) and online version (E-ISSN 2092-9293). It covers all disciplines of genetics and genomics from prokaryotes to eukaryotes from fundamental heredity to molecular aspects. The articles can be reviews, research articles, and short communications.