Staphylococcus epidermidis alters macrophage polarization and phagocytic uptake by extracellular DNA release in vitro.

IF 7.8 1区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY npj Biofilms and Microbiomes Pub Date : 2024-11-20 DOI:10.1038/s41522-024-00604-7

Samira Weißelberg, Anna Both, Antonio Virgilio Failla, Jiabin Huang, Stefan Linder, Denise Ohnezeit, Patricia Bartsch, Martin Aepfelbacher, Holger Rohde

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Abstract

Biofilm formation shields Staphylococcus epidermidis from host defense mechanisms, contributing to chronic implant infections. Using wild-type S. epidermidis 1457, a PIA-negative mutant (1457-M10), and an eDNA-negative mutant (1457ΔatlE), this study examined the influence of biofilm matrix components on human monocyte-derived macrophage (hMDM) interactions. The wild-type strain was resistant to phagocytosis and induced an anti-inflammatory response in hMDMs, while both mutants were more susceptible to phagocytosis and triggered a pro-inflammatory response. Removing eDNA from the 1457 biofilm matrix increased hMDM uptake and a pro-inflammatory reaction, whereas adding eDNA to the 1457ΔatlE mutant reduced phagocytosis and promoted an anti-inflammatory response. Inhibiting TLR9 enhanced bacterial uptake and induced a pro-inflammatory response in hMDMs exposed to wild-type S. epidermidis. This study highlights the critical role of eDNA in immune evasion and the central role of TLR9 in modulating macrophage responses, advancing the understanding of implant infections.

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表皮葡萄球菌在体外通过释放细胞外 DNA 改变巨噬细胞的极化和吞噬作用。

生物膜的形成使表皮葡萄球菌免受宿主防御机制的影响，从而导致慢性植入感染。本研究利用野生型表皮葡萄球菌 1457、PIA 阴性突变体（1457-M10）和 eDNA 阴性突变体（1457ΔatlE），研究了生物膜基质成分对人类单核细胞衍生巨噬细胞（hMDM）相互作用的影响。野生型菌株对吞噬作用有抵抗力，并能诱导 hMDMs 产生抗炎反应，而两种突变体对吞噬作用更敏感，并能引发促炎反应。从1457生物膜基质中移除eDNA会增加hMDM的吸收和促炎反应，而在1457ΔatlE突变体中添加eDNA会减少吞噬作用并促进抗炎反应。在暴露于野生型表皮葡萄球菌的 hMDMs 中，抑制 TLR9 会增强细菌摄取并诱发促炎反应。这项研究强调了 eDNA 在免疫逃避中的关键作用以及 TLR9 在调节巨噬细胞反应中的核心作用，从而加深了人们对种植感染的理解。

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来源期刊

npj Biofilms and Microbiomes Immunology and Microbiology-Microbiology

CiteScore

12.10

自引率

3.30%

发文量

审稿时长

9 weeks

期刊介绍： npj Biofilms and Microbiomes is a comprehensive platform that promotes research on biofilms and microbiomes across various scientific disciplines. The journal facilitates cross-disciplinary discussions to enhance our understanding of the biology, ecology, and communal functions of biofilms, populations, and communities. It also focuses on applications in the medical, environmental, and engineering domains. The scope of the journal encompasses all aspects of the field, ranging from cell-cell communication and single cell interactions to the microbiomes of humans, animals, plants, and natural and built environments. The journal also welcomes research on the virome, phageome, mycome, and fungome. It publishes both applied science and theoretical work. As an open access and interdisciplinary journal, its primary goal is to publish significant scientific advancements in microbial biofilms and microbiomes. The journal enables discussions that span multiple disciplines and contributes to our understanding of the social behavior of microbial biofilm populations and communities, and their impact on life, human health, and the environment.