Rapid and simple fluorescent detection of chlorogenic acid in Aidi injection using aggregation-induced emission (AIE) nanoclusters

IF 3.1 3区医学 Q2 CHEMISTRY, ANALYTICAL Journal of pharmaceutical and biomedical analysis Pub Date : 2024-11-16 DOI:10.1016/j.jpba.2024.116570

Pengwei Zhao , Zhixuan Song , Yunhan Li , Xiaorui Liu , Zhengjin Jiang , Qing Zhu , Jia-Huan Qu

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Abstract

Chlorogenic acid (CGA) is a key component in Aidi injection, known for its anti-cancer properties and ability to reduce toxicity. Therefore, accurate detection of CGA levels in Aidi injection is essential for monitoring therapeutic efficacy and minimizing adverse effects. This study presents a rapid and simple fluorescent method for detecting CGA in Aidi injection using aggregation-induced emission (AIE) nanoclusters, i.e. D(-)-penicillamine (DPA)-capped bimetallic gold/copper nanoclusters (DPA-Au/CuNCs). Upon the addition of CGA, the aggregation state of DPA-Au/CuNCs was disrupted through hydrogen bond formation and ligand exchange, leading to fluorescence quenching. The prepared DPA-Au/CuNCs exhibited a rapid response time of 0.5 min, and demonstrated good sensitivity for CGA, with a limit of detection of 3.75 μg/mL, and a linear detection range of 12.5–200 μg/mL. This method was successfully applied for the analysis of CGA in Aidi injection and plasma with good recovery rates and minimal matrix effect, highlighting its potential for the applications in pharmaceutical products and clinical samples.

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利用聚集诱导发射（AIE）纳米团簇对艾迪注射液中的绿原酸进行快速简单的荧光检测。

绿原酸（CGA）是爱迪注射液中的一种关键成分，因其抗癌特性和降低毒性的能力而闻名。因此，准确检测爱迪注射液中的 CGA 含量对于监测疗效和减少不良反应至关重要。本研究提出了一种利用聚集诱导发射（AIE）纳米团簇（即 D(-)-penicillamine (DPA) -capped bimetallic gold/copper nanoclusters (DPA-Au/CuNCs)）检测艾迪注射液中 CGA 的快速而简单的荧光方法。加入 CGA 后，DPA-Au/CuNCs 的聚集状态通过氢键形成和配体交换被破坏，从而导致荧光淬灭。所制备的 DPA-Au/CuNCs 的快速反应时间为 0.5 分钟，对 CGA 具有良好的灵敏度，检测限为 3.75 μg/mL，线性检测范围为 12.5-200 μg/mL。该方法成功地应用于艾迪注射液和血浆中CGA的分析，回收率高，基质效应小，突出了其在医药产品和临床样品中的应用潜力。

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来源期刊

Journal of pharmaceutical and biomedical analysis 医学-分析化学

CiteScore

6.70

自引率

5.90%

发文量

588

审稿时长

37 days

期刊介绍： This journal is an international medium directed towards the needs of academic, clinical, government and industrial analysis by publishing original research reports and critical reviews on pharmaceutical and biomedical analysis. It covers the interdisciplinary aspects of analysis in the pharmaceutical, biomedical and clinical sciences, including developments in analytical methodology, instrumentation, computation and interpretation. Submissions on novel applications focusing on drug purity and stability studies, pharmacokinetics, therapeutic monitoring, metabolic profiling; drug-related aspects of analytical biochemistry and forensic toxicology; quality assurance in the pharmaceutical industry are also welcome. Studies from areas of well established and poorly selective methods, such as UV-VIS spectrophotometry (including derivative and multi-wavelength measurements), basic electroanalytical (potentiometric, polarographic and voltammetric) methods, fluorimetry, flow-injection analysis, etc. are accepted for publication in exceptional cases only, if a unique and substantial advantage over presently known systems is demonstrated. The same applies to the assay of simple drug formulations by any kind of methods and the determination of drugs in biological samples based merely on spiked samples. Drug purity/stability studies should contain information on the structure elucidation of the impurities/degradants.