ZnO nanoparticles enhances cadmium tolerance by modulating N6-methyladenosine (m6A) level of stress-responsive genes NRT1 and GM35E in vegetable soybean.
{"title":"ZnO nanoparticles enhances cadmium tolerance by modulating N6-methyladenosine (m6A) level of stress-responsive genes NRT1 and GM35E in vegetable soybean.","authors":"Bin Wang, Ruiqiu Fang","doi":"10.1016/j.plaphy.2024.109303","DOIUrl":null,"url":null,"abstract":"<p><p>Cadmium (Cd) is a hazardous heavy metal pollutant that poses significant risks to agricultural production and human health. Nanoparticles (NPs) can alleviate the effects of cadmium on crops by regulating the expression of stress-responsive genes, however, the mechanism of regulation is unknown. N6-methyladenosine (m6A) is a prevalent RNA modification, which determines the expression level of RNA. In this study, we performed m6A methylome analysis and gene editing to investigate the regulation of stress-responsive genes by m6A under Cd stress with ZnO nanoparticles (ZnO NPs). Firstly, we identified 16 differentially expressed genes (DEGs) with differential m6A-modification by m6A methylome seq, which included 6 stress-responsive genes. ZnO NPs treatment reduced the m6A level and stabilized the mRNAs of these stress-responsive genes. Then, we utilized the in vivo m6A modification tool, Plant m6A Editors (PMEs), specifically reduced the m6A level of NRT1 and GM35E in transgenic plants. The expression of NRT1 and GM35E was increased, and plants carrying PMEs-NRT1 and PMEs-GM35E showed stronger Cd tolerance than control. Therefore, we can conclude that NPs enhance Cd tolerance in plants by regulating the m6A methylation level of stress-responsive genes such as NRT1 and GM35E, which ultimately affects the expression of these genes. This study proposed a post-transcriptional regulatory network in vegetable soybean roots responding to Cd with ZnO NPs, potentially offering new insights into gene manipulation for controlling low Cd accumulation in crops.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"109303"},"PeriodicalIF":6.1000,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Physiology and Biochemistry","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1016/j.plaphy.2024.109303","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Cadmium (Cd) is a hazardous heavy metal pollutant that poses significant risks to agricultural production and human health. Nanoparticles (NPs) can alleviate the effects of cadmium on crops by regulating the expression of stress-responsive genes, however, the mechanism of regulation is unknown. N6-methyladenosine (m6A) is a prevalent RNA modification, which determines the expression level of RNA. In this study, we performed m6A methylome analysis and gene editing to investigate the regulation of stress-responsive genes by m6A under Cd stress with ZnO nanoparticles (ZnO NPs). Firstly, we identified 16 differentially expressed genes (DEGs) with differential m6A-modification by m6A methylome seq, which included 6 stress-responsive genes. ZnO NPs treatment reduced the m6A level and stabilized the mRNAs of these stress-responsive genes. Then, we utilized the in vivo m6A modification tool, Plant m6A Editors (PMEs), specifically reduced the m6A level of NRT1 and GM35E in transgenic plants. The expression of NRT1 and GM35E was increased, and plants carrying PMEs-NRT1 and PMEs-GM35E showed stronger Cd tolerance than control. Therefore, we can conclude that NPs enhance Cd tolerance in plants by regulating the m6A methylation level of stress-responsive genes such as NRT1 and GM35E, which ultimately affects the expression of these genes. This study proposed a post-transcriptional regulatory network in vegetable soybean roots responding to Cd with ZnO NPs, potentially offering new insights into gene manipulation for controlling low Cd accumulation in crops.
期刊介绍：
Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement.
Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB.
Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
