Detection of microRNA-21 based on smartly designed ratiometric electrochemical sensor and dual-signal amplification

IF 5.7 2区 化学 Q1 CHEMISTRY, ANALYTICAL Analytica Chimica Acta Pub Date : 2024-11-22 DOI:10.1016/j.aca.2024.343444
Honglei Shao, Xingming Xue, Zhaomei Sun, Xiangjiang Zheng, Pengfei Shi
{"title":"Detection of microRNA-21 based on smartly designed ratiometric electrochemical sensor and dual-signal amplification","authors":"Honglei Shao, Xingming Xue, Zhaomei Sun, Xiangjiang Zheng, Pengfei Shi","doi":"10.1016/j.aca.2024.343444","DOIUrl":null,"url":null,"abstract":"MicroRNA(miRNA) serves as an effective and viable biomarker for early diagnosis and monitoring of cancer disorders. It is highly expressed in tumor cells, including lung cancer, liver cancer and lymphoma. Herein, we propose a ratiometric electrochemical sensor for ultrasensitive detection of miRNA-21 using dual signal amplification, hybridization chain reaction and Exo III assisted-amplification. Methylene blue (MB) and Hemin are chosen as two electrochemical species. Then the ratiometric electrochemical sensor were developed, which showed favorable performance of miRNA-21 detection, and exhibited a detection concentration range from 1 fM to 10 nM. Notably, the limit of detection for this biosensor was 0.15 fM. Overall, this strategy for miRNA detection holds significant promise for early cancer screening.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"61 1","pages":""},"PeriodicalIF":5.7000,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytica Chimica Acta","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1016/j.aca.2024.343444","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0

Abstract

MicroRNA(miRNA) serves as an effective and viable biomarker for early diagnosis and monitoring of cancer disorders. It is highly expressed in tumor cells, including lung cancer, liver cancer and lymphoma. Herein, we propose a ratiometric electrochemical sensor for ultrasensitive detection of miRNA-21 using dual signal amplification, hybridization chain reaction and Exo III assisted-amplification. Methylene blue (MB) and Hemin are chosen as two electrochemical species. Then the ratiometric electrochemical sensor were developed, which showed favorable performance of miRNA-21 detection, and exhibited a detection concentration range from 1 fM to 10 nM. Notably, the limit of detection for this biosensor was 0.15 fM. Overall, this strategy for miRNA detection holds significant promise for early cancer screening.

Abstract Image

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
基于智能设计的比率电化学传感器和双信号放大技术检测 microRNA-21
微小核糖核酸(miRNA)是早期诊断和监测癌症疾病的有效、可行的生物标志物。它在肺癌、肝癌和淋巴瘤等肿瘤细胞中高度表达。在此,我们提出了一种比率电化学传感器,利用双信号放大、杂交链反应和 Exo III 辅助放大技术对 miRNA-21 进行超灵敏检测。我们选择亚甲基蓝(MB)和血红蛋白作为两种电化学物质。结果表明,该传感器具有良好的 miRNA-21 检测性能,检测浓度范围为 1 fM 至 10 nM。值得注意的是,这种生物传感器的检测限为 0.15 fM。总之,这种 miRNA 检测策略在早期癌症筛查方面前景广阔。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Analytica Chimica Acta
Analytica Chimica Acta 化学-分析化学
CiteScore
10.40
自引率
6.50%
发文量
1081
审稿时长
38 days
期刊介绍: Analytica Chimica Acta has an open access mirror journal Analytica Chimica Acta: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. Analytica Chimica Acta provides a forum for the rapid publication of original research, and critical, comprehensive reviews dealing with all aspects of fundamental and applied modern analytical chemistry. The journal welcomes the submission of research papers which report studies concerning the development of new and significant analytical methodologies. In determining the suitability of submitted articles for publication, particular scrutiny will be placed on the degree of novelty and impact of the research and the extent to which it adds to the existing body of knowledge in analytical chemistry.
期刊最新文献
Review on activity-based detection of doping substances and growth promotors in biological matrices: do bioassays deserve a place in control programs? Look but don't touch: Non-invasive chemical analysis of organic paint binders - A review. Spatiotemporal metabolic mapping of ex-situ preserved hearts subjected to dialysis by integration of bio-SPME sampling with non-targeted metabolipidomic profiling Corrigendum to “A liquid crystal-decorated aptasensing gadget for rapid monitoring of A549 cells: Future portable test kit for lung cancer diagnosis” [Anal. Chim. Acta. 1330 (2024), 343276-343285] Corrigendum to “A label-free liquid crystal-assisted aptasensor for trace level detection of tobramycin in milk and chicken egg samples” [Anal. Chim. Acta, 1236 (2022), 340588]
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1