Engineering Thermoresponsive Enzyme–Polymer Conjugates via Glycan-Selective In Situ Polymerization for Recyclable Homogeneous Biocatalysis

IF 5.4 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Biomacromolecules Pub Date : 2024-12-09 DOI:10.1021/acs.biomac.4c01220

Huiru Wang , Shanyun Ma , Muyan Diao , Li Wenhui , Min Huan , Xiaofang Sun , Yuanzi Wu

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Abstract

Enzymes are crucial for various technological applications, but their inherent instability and short lifespan pose challenges. This study presents facile immobilized enzyme technology with the development of thermoresponsive enzyme–polymer conjugates (EPCs), using glucose oxidase (GOx) as a model enzyme, to address these limitations. By conjugating heteropolymers to the glycan moieties of GOx through a precise in situ polymerization process, we could modulate the lower critical solution temperature of the EPCs, enhancing enzyme performance without compromising its active site. The EPCs demonstrate a switchable behavior that facilitates efficient homogeneous catalysis and easy heterogeneous separation, reducing costs and environmental impact in industrial applications. Our strategy presents a versatile platform for creating efficient biocatalysts with tunable properties, marking a step forward in sustainable and cost-effective bioprocessing.

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通过聚糖选择性原位聚合作用设计热致伸缩性酶-聚合物共轭物，用于可回收的均相生物催化。

酶对各种技术应用至关重要，但其固有的不稳定性和短暂的生命周期带来了挑战。本研究以葡萄糖氧化酶（GOx）为模型酶，通过开发热致伸缩性酶-聚合物共轭物（EPCs），提出了简便的固定化酶技术，以解决这些局限性。通过精确的原位聚合过程将杂多聚合物与 GOx 的糖分子共轭，我们可以调节 EPCs 较低的临界溶液温度，在不影响其活性位点的情况下提高酶的性能。EPCs 具有可切换的特性，可促进高效的均相催化和简易的异相分离，从而降低工业应用中的成本和对环境的影响。我们的策略为创造具有可调特性的高效生物催化剂提供了一个多功能平台，标志着在可持续和具有成本效益的生物加工领域又向前迈进了一步。

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来源期刊

Biomacromolecules 化学-高分子科学

CiteScore

10.60

自引率

4.80%

发文量

417

审稿时长

1.6 months

期刊介绍： Biomacromolecules is a leading forum for the dissemination of cutting-edge research at the interface of polymer science and biology. Submissions to Biomacromolecules should contain strong elements of innovation in terms of macromolecular design, synthesis and characterization, or in the application of polymer materials to biology and medicine. Topics covered by Biomacromolecules include, but are not exclusively limited to: sustainable polymers, polymers based on natural and renewable resources, degradable polymers, polymer conjugates, polymeric drugs, polymers in biocatalysis, biomacromolecular assembly, biomimetic polymers, polymer-biomineral hybrids, biomimetic-polymer processing, polymer recycling, bioactive polymer surfaces, original polymer design for biomedical applications such as immunotherapy, drug delivery, gene delivery, antimicrobial applications, diagnostic imaging and biosensing, polymers in tissue engineering and regenerative medicine, polymeric scaffolds and hydrogels for cell culture and delivery.