The impact of skinfold thickness and exercise intensity on the reliability of NIRS in the vastus lateralis.

IF 2.7 3区医学 Q2 PHYSIOLOGY European Journal of Applied Physiology Pub Date : 2025-04-01 Epub Date: 2024-11-21 DOI:10.1007/s00421-024-05654-5

Lena Stuer, Massimo Teso, Alessandro L Colosio, Maura Loi, Patrick Mucci, Silvia Pogliaghi, Jan Boone, Kevin Caen

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Abstract

Purpose: The aims of this study were (1) to assess the test-retest reliability of the primary near infrared spectroscopy (NIRS) variables (i.e., StO₂, T[Hb], [HbO₂] and [HHb]) during cycling and (2) to investigate potential influences of exercise intensity and adipose tissue thickness (ATT) on this reliability.

Methods: 21 men and 20 women completed twelve constant work rate tests (6 min) at six different exercise intensities with each intensity performed twice. NIRS variables were measured at the vastus lateralis. The coefficient of variance (CV%), the intraclass correlation coefficient (ICC), mean bias and limits of agreement (LoA) were determined for reliability purposes.

Results: The reliability of baseline values were acceptable to very good (CV% range: 5.83 - 21.96%). The reliability of end-values (CV% range: 0.02 - 25.02%, ICC range: 0.0 - 0.935) and amplitudes (CV% range: 0.46 - 5099%, ICC range: 0.0 - 0.887) were more variable. In general, the mean biases of end-values and amplitudes showed wide limits of agreement. A homogeneous influence of exercise intensity on reliability could not be established but reliability measures appeared to be lower in people with a lower skinfold thickness. Moreover, the NIRS signals decreased with increasing ATT but stabilized upon reaching a cut-off of 8 mm ATT. In addition, ATT did have a significant influence on [HHb] amplitude. In participants with ATT < 8 mm, higher amplitudes were observed with increasing intensity whereas in participants with ATT > 8 mm, there were no differences between the intensities.

Conclusion: The study reveals variable results with regards to reliability and there was no consistent influence of exercise intensity on reliability. Participants with a lower skinfold thickness showed stronger reliability. Moreover, NIRS signals decrease when ATT exceeds 8 mm. Careful consideration is necessary when interpreting NIRS signals in such cases.

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皮褶厚度和运动强度对外侧肌近红外光谱可靠性的影响。

目的：本研究的目的是：（1）评估骑自行车时主要近红外光谱（NIRS）变量（即 StO2、T[Hb]、[HbO2] 和 [HHb]）的测试-再测试可靠性；（2）研究运动强度和脂肪组织厚度（ATT）对可靠性的潜在影响。方法：21 名男性和 20 名女性在 6 种不同的运动强度下完成 12 次恒定工作速率测试（6 分钟），每次强度测试进行两次。在阔筋膜外侧测量 NIRS 变量。测定了方差系数 (CV%)、类内相关系数 (ICC)、平均偏差和一致性限值 (LoA)，以获得可靠性：基线值的可靠性从可接受到非常好（CV% 范围：5.83 - 21.96%）。终值（CV%范围：0.02 - 25.02%，ICC范围：0.0 - 0.935）和振幅（CV%范围：0.46 - 5099%，ICC范围：0.0 - 0.887）的可靠性变化较大。总体而言，终点值和振幅的平均偏差显示出较大的一致性。运动强度对可靠性的影响不尽相同，但皮褶厚度较低的人的可靠性似乎较低。此外，NIRS 信号随着 ATT 的增加而降低，但在达到 8 mm ATT 临界值后趋于稳定。此外，ATT对[HHb]振幅有显著影响。在 ATT 为 8 毫米的参与者中，强度之间没有差异：研究显示，可靠性方面的结果各不相同，运动强度对可靠性没有一致的影响。皮褶厚度较低的参与者表现出更强的可靠性。此外，当 ATT 超过 8 毫米时，NIRS 信号会降低。在这种情况下解释近红外光谱信号时必须谨慎。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

European Journal of Applied Physiology 医学-生理学

CiteScore

6.00

自引率

6.70%

发文量

227

审稿时长

3 months

期刊介绍： The European Journal of Applied Physiology (EJAP) aims to promote mechanistic advances in human integrative and translational physiology. Physiology is viewed broadly, having overlapping context with related disciplines such as biomechanics, biochemistry, endocrinology, ergonomics, immunology, motor control, and nutrition. EJAP welcomes studies dealing with physical exercise, training and performance. Studies addressing physiological mechanisms are preferred over descriptive studies. Papers dealing with animal models or pathophysiological conditions are not excluded from consideration, but must be clearly relevant to human physiology.