{"title":"Correlations of Nasal Microbiome with Allergic Rhinitis and Its Symptoms Severity in Children Progression.","authors":"Zhipan Teng, Qi Li, Xiao-Fei Shen","doi":"10.2147/JAA.S489384","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>Human microbiome is involved in the pathogenesis of allergic diseases, but the impact of nasal microbiota on allergic rhinitis (AR) symptoms severity has not been evaluated. This study aimed to characterize nasal microbiome in AR children and its correlations with AR symptoms.</p><p><strong>Methods: </strong>According to diagnostic guidelines for AR, 45 AR children and 40 healthy subjects were recruited from July to August in 2023. Based on the total score of nasal symptoms (TNSS), the 45 AR patients were divided into a mild AR group (MAR) (n = 16) and a moderate or severe AR group (MSAR) (n = 29). Nasal swabs were collected for microbiome analysis using 16S-rDNA sequencing.</p><p><strong>Results: </strong>The Simpson and Shannon indices were significantly higher in the AR group compared to the health control group, indicating an increase of nasal microbiota at the species evenness level in AR children. Moreover, the species evenness was significantly increased in the MSAR group compared to the MAR group. <i>Staphylococcus</i> (member of the <i>Firmicutes</i> phylum) was significantly dominant in the AR group, but <i>Moraxella</i> (member of the <i>Proteobacteria</i> phylum) was significantly dominant in the CG group. The LEfSe analysis showed that the mean relative abundances of <i>Ralstonia</i> in the MSAR group was higher than that in the MAR group. Meanwhile, the abundance divided by <i>Ralstonia</i> of Spearman correlation coefficients was positively correlated with the TNSS of AR symptoms (r = 0.4, <i>P</i> = 0.009).</p><p><strong>Conclusion: </strong>The elevation of species evenness in nasal microbiome was likely related to the aggravation of AR symptoms. The <i>Ralstonia</i> may play a pro-inflammatory role in AR.</p>","PeriodicalId":15079,"journal":{"name":"Journal of Asthma and Allergy","volume":"17 ","pages":"1187-1196"},"PeriodicalIF":3.7000,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11578923/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Asthma and Allergy","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.2147/JAA.S489384","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"ALLERGY","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: Human microbiome is involved in the pathogenesis of allergic diseases, but the impact of nasal microbiota on allergic rhinitis (AR) symptoms severity has not been evaluated. This study aimed to characterize nasal microbiome in AR children and its correlations with AR symptoms.
Methods: According to diagnostic guidelines for AR, 45 AR children and 40 healthy subjects were recruited from July to August in 2023. Based on the total score of nasal symptoms (TNSS), the 45 AR patients were divided into a mild AR group (MAR) (n = 16) and a moderate or severe AR group (MSAR) (n = 29). Nasal swabs were collected for microbiome analysis using 16S-rDNA sequencing.
Results: The Simpson and Shannon indices were significantly higher in the AR group compared to the health control group, indicating an increase of nasal microbiota at the species evenness level in AR children. Moreover, the species evenness was significantly increased in the MSAR group compared to the MAR group. Staphylococcus (member of the Firmicutes phylum) was significantly dominant in the AR group, but Moraxella (member of the Proteobacteria phylum) was significantly dominant in the CG group. The LEfSe analysis showed that the mean relative abundances of Ralstonia in the MSAR group was higher than that in the MAR group. Meanwhile, the abundance divided by Ralstonia of Spearman correlation coefficients was positively correlated with the TNSS of AR symptoms (r = 0.4, P = 0.009).
Conclusion: The elevation of species evenness in nasal microbiome was likely related to the aggravation of AR symptoms. The Ralstonia may play a pro-inflammatory role in AR.
目的:人类微生物群参与了过敏性疾病的发病机制,但鼻腔微生物群对过敏性鼻炎(AR)症状严重程度的影响尚未得到评估。本研究旨在描述 AR 儿童鼻腔微生物组的特征及其与 AR 症状的相关性:方法:根据AR诊断指南,于2023年7月至8月招募了45名AR儿童和40名健康受试者。根据鼻部症状总分(TNSS),45名AR患者被分为轻度AR组(MAR)(16人)和中度或重度AR组(MSAR)(29人)。采集鼻拭子,利用 16S-rDNA 测序技术进行微生物组分析:结果:与健康对照组相比,AR 组的辛普森指数和香农指数明显较高,这表明 AR 儿童鼻腔微生物群的物种均匀度有所增加。此外,与MAR组相比,澳门特别行政区组的物种均匀度也明显增加。葡萄球菌(固着菌门成员)在 AR 组明显占优势,但莫拉菌(变形菌门成员)在 CG 组明显占优势。LEfSe 分析表明,澳门星际娱乐网址组中 Ralstonia 的平均相对丰度高于 MAR 组。同时,斯皮尔曼相关系数的 Ralstonia 分丰度与 AR 症状的 TNSS 呈正相关(r = 0.4,P = 0.009):结论:鼻腔微生物组中物种均匀度的升高可能与 AR 症状的加重有关。Ralstonia可能在AR中起到促炎作用。
期刊介绍:
An international, peer-reviewed journal publishing original research, reports, editorials and commentaries on the following topics: Asthma; Pulmonary physiology; Asthma related clinical health; Clinical immunology and the immunological basis of disease; Pharmacological interventions and new therapies.
Although the main focus of the journal will be to publish research and clinical results in humans, preclinical, animal and in vitro studies will be published where they shed light on disease processes and potential new therapies.