Laboratory practices for the diagnosis and management of mucormycosis in France, 2024

IF 2.2 4区医学 Q3 MYCOLOGY Journal de mycologie medicale Pub Date : 2024-11-14 DOI:10.1016/j.mycmed.2024.101520

Laurence Millon , Françoise Botterel , Julie Bonhomme , Stéphane Valot , Philippe Poirier , Marie-Fleur Durieux , Jeanne Bigot , Guillaume Desoubeaux , Adélaïde Chesnais , Florent Morio , Marc Pihet , Kévin Brunet , Anne-Pauline Bellanger , Sébastien Imbert , Gilles Nevez , Solène Le Gal , Nathalie Bourgeois , Anne Debourgogne , Marjorie Cornu , Florence Persat , Eric Dannaoui

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Abstract

This study investigates the diagnostic practices for mucormycosis among 30 French University Hospital mycology laboratories, in 2024. All laboratories perform both direct examination and culture, with fluorescent brighteners being the most commonly used method for direct examination. While 77 % of the participating laboratories routinely identify Mucorales to the species level, with 70 % having adopted Mucorales-specific quantitative PCR, primarily for the diagnosis of invasive fungal infections. Antifungal susceptibility testing practices varied between centers, with 36.7 % of laboratories consistently performing these tests, primarily using gradient concentration strips. Amphotericin B, posaconazole, and isavuconazole were the most frequently tested antifungals. These findings highlight variations in laboratory practices and emphasize the importance of establishing uniform diagnostic and susceptibility testing methods to optimize mucormycosis management.

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2024 年法国粘孢子菌病诊断和管理的实验室实践。

本研究调查了 2024 年法国 30 所大学医院真菌学实验室对粘孢子菌病的诊断方法。所有实验室都进行直接检查和培养，荧光增白剂是最常用的直接检查方法。77% 的参与实验室会对真菌菌种进行常规鉴定，70% 的实验室采用了真菌特异性定量 PCR 技术，主要用于诊断侵袭性真菌感染。各中心的抗真菌药敏试验方法不尽相同，36.7%的实验室一直在进行这些试验，主要使用梯度浓度条。两性霉素 B、泊沙康唑和异武康唑是最常检测的抗真菌药物。这些发现凸显了实验室实践中的差异，并强调了建立统一的诊断和药敏试验方法对优化粘孢子菌病管理的重要性。

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来源期刊

Journal de mycologie medicale 医学-真菌学

CiteScore

5.10

自引率

2.80%

发文量

审稿时长

6-12 weeks

期刊介绍： The Journal de Mycologie Medicale / Journal of Medical Mycology (JMM) publishes in English works dealing with human and animal mycology. The subjects treated are focused in particular on clinical, diagnostic, epidemiological, immunological, medical, pathological, preventive or therapeutic aspects of mycoses. Also covered are basic aspects linked primarily with morphology (electronic and photonic microscopy), physiology, biochemistry, cellular and molecular biology, immunochemistry, genetics, taxonomy or phylogeny of pathogenic or opportunistic fungi and actinomycetes in humans or animals. Studies of natural products showing inhibitory activity against pathogenic fungi cannot be considered without chemical characterization and identification of the compounds responsible for the inhibitory activity. JMM publishes (guest) editorials, original articles, reviews (and minireviews), case reports, technical notes, letters to the editor and information. Only clinical cases with real originality (new species, new clinical present action, new geographical localization, etc.), and fully documented (identification methods, results, etc.), will be considered. Under no circumstances does the journal guarantee publication before the editorial board makes its final decision. The journal is indexed in the main international databases and is accessible worldwide through the ScienceDirect and ClinicalKey platforms.