Occurrence of Salmonella enterica in faecal sludge from Nigeria and genetic relatedness with strains associated with human infections in Africa.

IF 3.2 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal of Applied Microbiology Pub Date : 2024-11-22 DOI:10.1093/jambio/lxae293

Abdurrahman Hassan Jibril, Anders Dalsgaard, Iruka N Okeke, Aliyu Ibrahim Musawa, John Elmerdahl Olsen

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Abstract

Aim: This study investigated occurrence of Salmonella in faecal sludge from public toilets in Nigeria, and genetic relatedness of strains which have been reported to cause human infection across Africa.

Methods and results: The study collected 150 human sludge from public toilets, and identified Salmonella through culture and PCR. Isolates were tested for antimicrobial susceptibility and sequenced using Illumina MiSeq. Draft sequences were compared with sequence data from Enterobase and Genbank. Twenty-four (16.0%) of sewage samples were positive for Salmonella [CI95 (10.2- 21.8)]. S. Give (sequence type (ST) 516), S. Seftenberg (ST-14), and S. Chester (ST-411) were the most prevalent serovars found in 45.8%, 16.7%, and 16.7% of samples, respectively. Most of the isolates were sensitive to the antimicrobials tested, only one isolate of S. Derby showed resistance to ampicillin and cefazolin. Notably, 91.7% of the strains had the aac (6)-Iaa gene, and point mutations in parC, gyrA and acrB. S. Chester showed genetic relatedness with strains from Benin Republic and South Africa.

Conclusion: There is genetic relatedness of present strains and those associated with human infections in Africa.

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尼日利亚粪便污泥中肠炎沙门氏菌的出现以及与非洲人类感染相关菌株的遗传相关性。

目的：本研究调查了尼日利亚公共厕所粪便污泥中沙门氏菌的发生情况，以及据报道在非洲各地引起人类感染的菌株的遗传亲缘关系：研究从公共厕所收集了 150 份人类污泥，并通过培养和 PCR 鉴定了沙门氏菌。对分离菌株进行抗菌药敏感性测试，并使用 Illumina MiSeq 进行测序。草拟序列与 Enterobase 和 Genbank 中的序列数据进行了比较。24 份（16.0%）污水样本对沙门氏菌呈阳性 [CI95 (10.2- 21.8)]。S. Give（序列类型 (ST) 516）、S. Seftenberg（ST-14）和 S. Chester（ST-411）是最常见的血清型，分别占样本的 45.8%、16.7% 和 16.7%。大多数分离株对测试的抗菌药敏感，只有一个德比沙雷氏菌分离株对氨苄西林和头孢唑啉有抗药性。值得注意的是，91.7%的菌株具有 aac (6)-Iaa 基因，以及 parC、gyrA 和 acrB 的点突变。切斯特氏菌与贝宁共和国和南非的菌株有遗传亲缘关系：结论：目前的菌株与非洲人类感染的菌株存在遗传亲缘关系。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Applied Microbiology 生物-生物工程与应用微生物

CiteScore

7.30

自引率

2.50%

发文量

427

审稿时长

2.7 months

期刊介绍： Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.