Christiana Kyvelidou, Sofia Haselrieder, Maria von Gierke, Johanna M Gostner, Wolfgang Biasio, Barbara Wirleitner, Christine Heufler, Bettina Toth, Susanne Hofer-Tollinger
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引用次数: 0
Abstract
Objective: To study the crosstalk between maternal immune cells and the developing embryo by investigating the immunogenic properties of human blastocyst spent media (SM) on dendritic cells.
Methods: In this prospective multicenter experimental study, human preimplantation embryo spent media were collected after blastocyst formation, grouped based on successful or unsuccessful implantation, and analyzed by protein array or used to stimulate monocyte derived dendritic cells (moDC). The immunomodulatory properties of SM on moDC were investigated by analyzing changes in phenotype, cytokine secretion, indoleamine 2,3-dioxygenase (IDO) activity, and ability to activate T cells.
Results: A plethora of cytokines and growth factors secreted from preimplantation embryos was detected. Exposure to embryo SM altered the phenotype of moDC in a manner dependent on the implantation outcome. Specifically, SM from non-implanted embryos increased the expression of co-stimulatory molecules and activation markers on moDC. Furthermore, SM treated dendritic cells secreted low levels of cytokines and growth factors and were able to stimulate naïve T cells. Activation of IDO was decreased in moDC after stimulation with SM.
Conclusions: Our findings show that human preimplantation embryos secrete an abundance of molecules with the ability to significantly affect and even regulate immune cells in their environment.
目的通过研究人胚泡废培养基(SM)对树突状细胞的免疫原性,研究母体免疫细胞与发育中胚胎之间的相互影响:在这项前瞻性多中心实验研究中,囊胚形成后收集植入前胚胎废培养基,根据植入成功与否进行分组,并通过蛋白质阵列分析或用于刺激单核细胞衍生树突状细胞(moDC)。通过分析表型、细胞因子分泌、吲哚胺 2,3-二氧酶(IDO)活性和激活 T 细胞能力的变化,研究了 SM 对 moDC 的免疫调节特性:结果:检测到植入前胚胎分泌大量细胞因子和生长因子。暴露于胚胎SM会改变moDC的表型,改变的方式取决于植入结果。具体来说,来自非植入胚胎的SM增加了moDC上共刺激分子和活化标记的表达。此外,经SM处理的树突状细胞分泌低水平的细胞因子和生长因子,并能刺激幼稚T细胞。经SM刺激后,moDC的IDO活化减少:我们的研究结果表明,人类植入前胚胎分泌的大量分子能够显著影响甚至调节其所处环境中的免疫细胞。
期刊介绍:
Reproductive Biology and Endocrinology publishes and disseminates high-quality results from excellent research in the reproductive sciences.
The journal publishes on topics covering gametogenesis, fertilization, early embryonic development, embryo-uterus interaction, reproductive development, pregnancy, uterine biology, endocrinology of reproduction, control of reproduction, reproductive immunology, neuroendocrinology, and veterinary and human reproductive medicine, including all vertebrate species.