A novel polysaccharide from Macadamia peel: Extraction, purification, structural characterization and antioxidant activity.

IF 3.3 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Journal of the Science of Food and Agriculture Pub Date : 2024-11-23 DOI:10.1002/jsfa.14040

Gangjun Guo, Wenting Xu, Jiarong Fu, Shangxuan Ma, Kechang Huang, Yuanmiao Wei, Yuexue Yang, Xiuhua Lan, Xiyong He

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Abstract

Background: Macadamia peels are the main by-product of postharvest treatment of the whole fresh fruit weight, they contain various bioactive substances, such as polysaccharides, phenols, flavonoids, phenolic acids, saponins, and other nutritional and functional components, that are known to have anti-tumor and anti-oxidation functions.

Results: Two purified polysaccharide fractions were obtained (MPP-1 and MPP-2) by extracting with ultrasonic-microwave-aided water extraction and purifying by with DEAE-52 and Sephadex G-50 columns, and then characterized using Fourier-transform infrared spectroscopy, scanning electron microscopy, atomic force microscopy and NMR, and the antioxidant activity was also investigated. The results indicated that MPP-1 and MPP-2 were mainly glucose, the molecular weight was 8.16 kDa and 7.73 kDa, respectively. Methylation with gas chromatography-mass spectrometry and NMR analyses confirmed that two fractions comprised of →4) -α-d- Glcp -(1→, →6) -β-d- Glcp -(1→ and →3,4) -β-d- Glcp -(1→) as the main chain. In addition, MPP-1 and MPP-2 polysaccharides showed significant antioxidant activity with respect to 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl group and reducing power, and MPP-2 demonstrated excellent antioxidant activities compared to MPP-1 with IC₅₀ values of 35.12, 18.82 and 40.12 μg mL^-1, respectively.

Conclusion: The novel polysaccharide, MPP-1 and MPP-2, mainly containing glucose, showed significant antioxidant activity with respect to DPPH, hydroxyl group and reducing power. This study has enhanced waste utilization and reduced environmental pollution, providing some inspiration for the reuse of the waste generated during agricultural production. It may bring good prospects for their use as antioxidants in functional foods. © 2024 Society of Chemical Industry.

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从澳洲坚果果皮中提取的一种新型多糖：提取、纯化、结构特征和抗氧化活性。

背景：澳洲坚果果皮是全鲜果采后处理的主要副产品，含有多种生物活性物质，如多糖、酚类、黄酮类、酚酸、皂苷等营养和功能成分，具有抗肿瘤和抗氧化作用：结果：通过超声波-微波辅助水提取、DEAE-52柱和Sephadex G-50柱纯化，得到了两种纯化的多糖组分（MPP-1和MPP-2），然后利用傅立叶变换红外光谱、扫描电子显微镜、原子力显微镜和核磁共振对其进行了表征，并对其抗氧化活性进行了研究。结果表明，MPP-1 和 MPP-2 主要为葡萄糖，分子量分别为 8.16 kDa 和 7.73 kDa。甲基化气相色谱-质谱和核磁共振分析证实，这两个馏分以 →4) -α-d- Glcp -(1→, →6) -β-d- Glcp -(1→ 和 →3,4) -β-d- Glcp -(1→) 为主链。此外，MPP-1 和 MPP-2 多糖在 1,1-二苯基-2-苦基肼（DPPH）、羟基和还原力方面表现出显著的抗氧化活性，MPP-2 与 MPP-1 相比表现出优异的抗氧化活性，IC50 值分别为 35.12、18.82 和 40.12 μg mL-1：以葡萄糖为主要成分的新型多糖 MPP-1 和 MPP-2 在 DPPH、羟基和还原力方面表现出显著的抗氧化活性。这项研究提高了废弃物的利用率，减少了环境污染，为农业生产过程中产生的废弃物的再利用提供了一些启示。这可能会为其在功能性食品中用作抗氧化剂带来良好的前景。© 2024 化学工业学会。

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.