Bioactive glass suspension hydrogel promotes wound healing by modulating fibroblasts.

IF 2.9 4区材料科学 Q3 MATERIALS SCIENCE, MULTIDISCIPLINARY Nanotechnology Pub Date : 2024-11-22 DOI:10.1088/1361-6528/ad9610

Kai Ma, Baichuan Chen, Xiaodi Xu, Lei Zhang, Rui Ruan, Hongbin Deng, Xiaolei Zhu, Hongda Zhu

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Abstract

The irritation and adhesion of wound healing biomaterials to wet wounds should be addressed for achieving effective wound healing. In this study, a stable multifunctional hydrogels (BGs/HA suspension gels) were prepared using superfine powder of bioactive glasses (BGs), the biocompatible materials hyaluronic acid (HA) and carbomer940, which had good adhesion and low irritation properties for use in moist complex wounds. The average particle size of BGs/HA suspension gels was 13.11 ± 0.29 μm, and the bioactive glass content was 15.8 ± 0.2% (m/m). The results of cell proliferation, cell migration, and immunofluorescence staining experiments showed that in the initial stage of wound healing, the ionic extract of BGs formulations promoted the proliferation and migration of L929 cells and induced the secretion of α-SMA and collagen I. In the final stage of repair, the ionic extract of the BGs formulation regulated the differentiation of fibroblast, which contributed to the reduction of pathological scar formation. In vivo experiments showed that the wound healing rate of BGs/HA suspension gels group exceeded higher than that of the conventional BGs superfine powder group. Although BGs/HA suspension gels were comparable to its commercially available counterpart (Dermlin paste) in promoting wound healing, it addressed the problem of localized irritation caused by the high pH and low adhesion of BGs products. This study confirmed the specific regulatory effect of BGs/HA suspension gels on L929 cells, which provided a reference for the clinical application of BGs in wound dressing.

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生物活性玻璃悬浮水凝胶通过调节成纤维细胞促进伤口愈合。

为实现有效的伤口愈合，应解决伤口愈合生物材料对湿伤口的刺激和粘附问题。本研究使用生物活性玻璃（BGs）超细粉末、生物相容性材料透明质酸（HA）和卡波姆 940 制备了一种稳定的多功能水凝胶（BGs/HA 悬浮凝胶），该凝胶具有良好的粘附性和低刺激性，可用于潮湿的复杂伤口。BGs/HA 悬浮凝胶的平均粒径为 13.11 ± 0.29 μm，生物活性玻璃含量为 15.8 ± 0.2%（m/m）。细胞增殖、细胞迁移和免疫荧光染色实验结果表明，在伤口愈合初期，BGs 配方的离子提取物促进了 L929 细胞的增殖和迁移，并诱导了 α-SMA 和胶原蛋白 I 的分泌；在修复的最后阶段，BGs 配方的离子提取物调节了成纤维细胞的分化，有助于减少病理性疤痕的形成。体内实验表明，BGs/HA 悬浮凝胶组的伤口愈合率高于传统 BGs 超细粉组。虽然 BGs/HA 悬浮凝胶在促进伤口愈合方面与市售同类产品（Dermlin 糊剂）不相上下，但它解决了 BGs 产品的高 pH 值和低粘附性导致的局部刺激问题。该研究证实了 BGs/HA 悬浮凝胶对 L929 细胞的特异性调节作用，为 BGs 在伤口敷料中的临床应用提供了参考。

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来源期刊

Nanotechnology 工程技术-材料科学：综合

CiteScore

7.10

自引率

5.70%

发文量

820

审稿时长

2.5 months

期刊介绍： The journal aims to publish papers at the forefront of nanoscale science and technology and especially those of an interdisciplinary nature. Here, nanotechnology is taken to include the ability to individually address, control, and modify structures, materials and devices with nanometre precision, and the synthesis of such structures into systems of micro- and macroscopic dimensions such as MEMS based devices. It encompasses the understanding of the fundamental physics, chemistry, biology and technology of nanometre-scale objects and how such objects can be used in the areas of computation, sensors, nanostructured materials and nano-biotechnology.