Bloom-forming planktonic Microcystis and benthic Oscillatoria-induced oxidative stress and inflammatory responses in juvenile silver carp and bighead carp.

Abstract

As global warming and water eutrophication, the multiple proliferation of harmful cyanobacteria can form algal blooms and cause serious ecological problems. In recent years, the large-scale and persistent cyanobacterial blooms occur frequently worldwide and have attracted widespread attention due to the harmful impacts. Among these harmful bloom-forming cyanobacteria, the ecological and toxicological impacts of planktonic cyanobacteria have been extensively studied. However, research on the ecological risks and adverse effects of harmful benthic cyanobacteria is lagging. Filter-feeding fish could suffer from more toxic stimuli than other fish due to their special feeding habits. To investigate and compare the complex toxic effects of different kinds of harmful cyanobacteria on fish, three different-sized (i.e. small, medium, and large) juvenile silver carp (Hypophthalmichthys molitrix) and bighead carp (Aristichthys nobilis) were exposed to cyanobacterial blooms-related density (1 × 10⁶ cells/mL) of Microcystis aeruginosa (i.e. generating microcystins) and Oscillatoria sp. (i.e. generating cylindrospermopsin) for 3 d, after which biomarkers of oxidative stress and inflammation in the liver of fish were detected. The silver carp and bighead carp can effectively ingest Microcystis cells but cannot effectively ingest Oscillatoria cells through the measurement of the levels of cyanotoxins. Both Microcystis and Oscillatoria cells can induce different levels of oxidative stress and inflammatory responses in the liver of these juvenile filter-feeding fish via altering the biochemical parameters of the antioxidant system (e.g. superoxide dismutase activity) and immune system (e.g. interleukin-1β level). Therefore, our research identified potential data gaps that how the different types of cyanobacteria induce toxic effects in the liver of juvenile filter-feeding fish in a short time. This study contributes to a better understanding of the short-term adverse effects of different cyanobacterial species on juvenile fish, suggesting that the benthic toxic cyanobacteria-induced ecological and health risks require further attention.