Characterization of a Novel Tn7-like Transposon Carrying blaDHA-1 in Providencia stuartii MF1 Isolated from Swine Wastewater.

IF 2.3 3区 生物学 Q3 MICROBIOLOGY Current Microbiology Pub Date : 2024-11-23 DOI:10.1007/s00284-024-03952-x
Shijie Ou, Xinyi Tian, Zhen Zhang, Lingjia Zhu, Rong Wang, Guangxiang Cao, Jiafang Fu, Peipei Zhang
{"title":"Characterization of a Novel Tn7-like Transposon Carrying blaDHA-1 in Providencia stuartii MF1 Isolated from Swine Wastewater.","authors":"Shijie Ou, Xinyi Tian, Zhen Zhang, Lingjia Zhu, Rong Wang, Guangxiang Cao, Jiafang Fu, Peipei Zhang","doi":"10.1007/s00284-024-03952-x","DOIUrl":null,"url":null,"abstract":"<p><p>Providencia stuartii is an emerging pathogen that causes nosocomial infections. In this study, a multidrug-resistant strain P. stuartii MF1 was isolated from swine wastewater. Comprehensive analysis of whole genome sequencing revealed that dozens of antibiotic resistance genes were found in MF1. A novel transposon Tn6450M which has high sequence identity to Tn6450 and the plasmid-borne Tn6765 from Proteus mirabilis was identified in the genome of MF1. Tn6450M was determined to be stably inserted into a new attTn7 site in the P. stuartii MF1 genome and contains the third-generation cephalosporins resistance-associated genes blaDHA-1. Intergeneric transmission of Tn6450 variants poses risks for the spread of antibiotic resistance genes.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 1","pages":"6"},"PeriodicalIF":2.3000,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s00284-024-03952-x","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Providencia stuartii is an emerging pathogen that causes nosocomial infections. In this study, a multidrug-resistant strain P. stuartii MF1 was isolated from swine wastewater. Comprehensive analysis of whole genome sequencing revealed that dozens of antibiotic resistance genes were found in MF1. A novel transposon Tn6450M which has high sequence identity to Tn6450 and the plasmid-borne Tn6765 from Proteus mirabilis was identified in the genome of MF1. Tn6450M was determined to be stably inserted into a new attTn7 site in the P. stuartii MF1 genome and contains the third-generation cephalosporins resistance-associated genes blaDHA-1. Intergeneric transmission of Tn6450 variants poses risks for the spread of antibiotic resistance genes.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
从猪废水中分离出的携带 blaDHA-1 的 Providencia stuartii MF1 的新型 Tn7 类转座子的特征。
普罗维登菌(Providencia stuartii)是一种新出现的病原体,可引起医院内感染。本研究从猪废水中分离出一株具有多重耐药性的 P. stuartii MF1。全基因组测序的综合分析表明,在 MF1 中发现了数十个抗生素耐药基因。在 MF1 的基因组中发现了一个新的转座子 Tn6450M,该转座子与 Tn6450 和奇异变形杆菌质粒上的 Tn6765 有很高的序列同一性。经测定,Tn6450M 被稳定地插入到 P. stuartii MF1 基因组中一个新的 attTn7 位点,并含有第三代头孢菌素耐药性相关基因 blaDHA-1。Tn6450变体的跨代传播为抗生素耐药基因的传播带来了风险。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Current Microbiology
Current Microbiology 生物-微生物学
CiteScore
4.80
自引率
3.80%
发文量
380
审稿时长
2.5 months
期刊介绍: Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment. Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas: physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.
期刊最新文献
Community Assembly Mechanisms of nirK- and nirS-type Denitrifying Bacteria in Sediments of Eutrophic Lake Taihu, China. Description of Massilia orientalis sp. nov., Isolated from Rhizosphere Soil of Ageratina adenophora. Exploring the Prebiotic Potential of Fermented Astragalus Polysaccharides on Gut Microbiota Regulation In Vitro. Photosynthetic Toxicological Effects of Organic Extracts from Zanthoxylum bungeanum Leaves on Controlling the Microcystis aeruginosa Blooms. Deciphering the Antimicrobial and Antibiofilm Efficiency of Thyme Essential Oil Encapsulated Zeolitic Imidazole Framework-8 Against Foodborne Pathogens.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1