Expression of two CD83 homologs in macrophage subpopulations isolated from the brain and kidney of ginbuna crucian carp.

IF 4.1 2区 农林科学 Q1 FISHERIES Fish & shellfish immunology Pub Date : 2024-11-21 DOI:10.1016/j.fsi.2024.110038
Trang Thu Tran, Takahiro Nagasawa, Miki Nakao, Tomonori Somamoto
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Abstract

There are numerous fish diseases that affect the central nervous system. However, few studies have investigated the immune cells and immunological responses of fish brains. Meanwhile, microglial cells, as the brain's first line of defense, play a vital role in neuroimmunology. Furthermore, CD83 is a co-stimulatory protein that regulates immunological responses and the activation of dendritic cells and macrophages. Although CD83 expression has been linked to the initial activation of microglia in various disease scenarios in mammals, its role in teleost microglial biology remains unclear. In a recent investigation, we discovered that Ginbuna crucian carp (Gb) contains two CD83 homologs (GbCD83 and GbCD83-L). In this study, we used modified procedures of mouse-based macrophage culture from the brain and kidney to identify that GbCD83-L is highly expressed by the brain microglia-like cells and kidney-resident macrophages (KRMs) at both the protein and gene levels. Interestingly, GbCD83-L was considerably elevated in the microglia-like cells and KRMs after 24 h of lipopolysaccharide stimulation. These findings provide the first evidence of CD83 as a potential marker for active microglia and KRMs in teleosts, thus making it a crucial regulator in fish neuroimmunology and a candidate for future immunomodulatory applications in aquaculture.

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从金鲫脑和肾中分离的巨噬细胞亚群中两种 CD83 同源物的表达。
影响中枢神经系统的鱼类疾病很多。然而,很少有研究调查鱼类大脑的免疫细胞和免疫反应。同时,小胶质细胞作为大脑的第一道防线,在神经免疫学中起着至关重要的作用。此外,CD83 是一种协同刺激蛋白,可调节免疫反应以及树突状细胞和巨噬细胞的活化。虽然 CD83 的表达与哺乳动物中各种疾病情况下小胶质细胞的初始激活有关,但它在远洋鱼类小胶质细胞生物学中的作用仍不清楚。在最近的一项调查中,我们发现银鲫(Gb)含有两个 CD83 同源物(GbCD83 和 GbCD83-L)。在这项研究中,我们使用改进的基于小鼠的脑和肾巨噬细胞培养程序,发现 GbCD83-L 在脑小胶质细胞和肾驻留巨噬细胞(KRMs)的蛋白和基因水平上都有高表达。有趣的是,在脂多糖刺激 24 小时后,小胶质细胞和肾驻留巨噬细胞中的 GbCD83-L 显著升高。这些发现首次证明了 CD83 是鱼类小胶质细胞和 KRMs 活跃的潜在标志物,从而使其成为鱼类神经免疫学中的一个重要调节因子,并成为未来水产养殖中免疫调节应用的候选者。
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来源期刊
Fish & shellfish immunology
Fish & shellfish immunology 农林科学-海洋与淡水生物学
CiteScore
7.50
自引率
19.10%
发文量
750
审稿时长
68 days
期刊介绍: Fish and Shellfish Immunology rapidly publishes high-quality, peer-refereed contributions in the expanding fields of fish and shellfish immunology. It presents studies on the basic mechanisms of both the specific and non-specific defense systems, the cells, tissues, and humoral factors involved, their dependence on environmental and intrinsic factors, response to pathogens, response to vaccination, and applied studies on the development of specific vaccines for use in the aquaculture industry.
期刊最新文献
Expression of two CD83 homologs in macrophage subpopulations isolated from the brain and kidney of ginbuna crucian carp. A preliminary investigation on the protective effects of β-glucan and mannan induced trained immunity in pufferfish Takifugu obscurus. Designing and evaluating a novel blood-brain barrier in vitro model of teleost for reproducing alterations in brain infecting. Expression profiling of antimicrobial peptides and immune-related genes in Nile tilapia following Pseudomonas putida infection and nano-titanium dioxide gel exposure. Identification and characterization of 16 tripartite motif-containing proteins from Takifugu obscurus.
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