Characterization of pecan PEBP family genes and the potential regulation role of CiPEBP-like1 in fatty acid synthesis.

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Plant Science Pub Date : 2024-11-21 DOI:10.1016/j.plantsci.2024.112326

Jiani Wang, Xinyao Liu, Qiaoyan Wang, Miao Shi, Caiyun Li, Huating Hou, Kean-Jin Lim, Zhengjia Wang, Zhengfu Yang

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引用次数: 0

Abstract

Phosphatidyl ethanolamine-binding protein (PEBP) plays important roles in plant growth and development. However, few studies have investigated the PEBP gene family in pecan (Carya illinoinensis), particularly the function of the PEBP-like subfamily. In this study, we identified 12 PEBP genes from the pecan genome and classified them into four subfamilies: MFT-like, FT-like, TFL1-like and PEBP-like. Multiple sequence alignment, gene structure, and conserved motif analyses indicated that pecan PEBP subfamily genes were highly conserved. Cis-element analysis revealed that many light responsive elements and plant hormone-responsive elements are found in CiPEBPs promoters. Additionally, RNA-seq and RT-qPCR showed that CiPEBP-like1 was highly expressed during kernel filling stage. GO and KEGG enrichment analysis further indicated that CiPEBP-like1 was involved in fatty acid biosynthesis and metabolism progress. Overexpression of CiPEBP-like1 led to earlier flowering and altered fatty acid composition in Arabidopsis seeds. RT-qPCR confirmed that CiPEBP-like1 promoted fatty acid synthesis by regulating the expression of key genes. Overall, this study contributes to a comprehensive understanding of the potential functions of the PEBP family genes and lay a foundation to modifying fatty acid composition in pecan kernel.

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山核桃 PEBP 家族基因的特征以及 CiPEBP-like1 在脂肪酸合成中的潜在调控作用。

磷脂酰乙醇胺结合蛋白（PEBP）在植物的生长和发育中发挥着重要作用。然而，很少有研究调查山核桃（Carya illinoinensis）中的 PEBP 基因家族，尤其是类 PEBP 亚家族的功能。在这项研究中，我们从山核桃基因组中鉴定了 12 个 PEBP 基因，并将其分为四个亚家族：MFT-like、FT-like、TFL1-like 和 PEBP-like。多重序列比对、基因结构和保守主题分析表明，山核桃PEBP亚家族基因高度保守。顺式元件分析表明，在 CiPEBPs 启动子中发现了许多光响应元件和植物激素响应元件。此外，RNA-seq 和 RT-qPCR 显示，CiPEBP-like1 在果仁充实期高表达。GO 和 KEGG 富集分析进一步表明，CiPEBP-like1 参与了脂肪酸的生物合成和代谢过程。过表达 CiPEBP-like1 会导致拟南芥种子提前开花并改变脂肪酸组成。RT-qPCR 证实，CiPEBP-like1 通过调控关键基因的表达促进了脂肪酸的合成。总之，这项研究有助于全面了解 PEBP 家族基因的潜在功能，并为改变山核桃核仁中的脂肪酸组成奠定基础。

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来源期刊

Plant Science 生物-生化与分子生物学

CiteScore

9.10

自引率

1.90%

发文量

322

审稿时长

33 days

期刊介绍： Plant Science will publish in the minimum of time, research manuscripts as well as commissioned reviews and commentaries recommended by its referees in all areas of experimental plant biology with emphasis in the broad areas of genomics, proteomics, biochemistry (including enzymology), physiology, cell biology, development, genetics, functional plant breeding, systems biology and the interaction of plants with the environment. Manuscripts for full consideration should be written concisely and essentially as a final report. The main criterion for publication is that the manuscript must contain original and significant insights that lead to a better understanding of fundamental plant biology. Papers centering on plant cell culture should be of interest to a wide audience and methods employed result in a substantial improvement over existing established techniques and approaches. Methods papers are welcome only when the technique(s) described is novel or provides a major advancement of established protocols.