Development of Zinc-Containing Chitosan/Gelatin Coatings with Immunomodulatory Effect for Soft Tissue Sealing around Dental Implants.

IF 4.4 4区医学 Q2 CELL & TISSUE ENGINEERING Tissue engineering and regenerative medicine Pub Date : 2024-11-23 DOI:10.1007/s13770-024-00680-y

Jing Han, Jorine G F Sanders, Lea Andrée, Bart A J A van Oirschot, Adelina S Plachokova, Jeroen J J P van den Beucken, Sander C G Leeuwenburgh, Fang Yang

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Abstract

Background: Soft tissue integration (STI) around dental implant abutments is a prerequisite to prevent bacterial invasion and achieve successful dental implant rehabilitation. However, peri-implant STI is a major challenge after dental abutment placement due to alterations in the immune microenvironment upon surgical dental implant installation.

Methods: Based on known immunomodulatory effects of zinc, we herein deposited zinc/chitosan/gelatin (Zn/CS/Gel) coatings onto titanium substrates to study their effect on macrophages. First, we exposed macrophages to cell culture media containing different zinc ion (Zn²⁺) concentrations. Next, we explored the immunomodulatory effect of Zn/CS/Gel coatings prepared via facile electrophoretic deposition (EPD).

Results: We found that Zn²⁺ effectively altered the secretome by reducing the secretion of pro-inflammatory and enhancing pro-regenerative cytokine secretion, particularly at a Zn²⁺ supplementation of approximately 37.5 μM. Zn/CS/Gel coatings released Zn²⁺ in a concentration range which effectively stimulated pro-regenerative macrophage polarization as demonstrated by M2 macrophage polarization. Additionally, the impact of these Zn²⁺-exposed macrophages on gingival fibroblasts incubated in conditioned medium showed stimulated adhesion, proliferation, and collagen secretion.

Conclusion: Our promising results suggest that controlled release of Zn²⁺ from Zn/CS/Gel coatings could be applied to immunomodulate peri-implant STI, and to enhance dental implant survival.

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开发具有免疫调节作用的含锌壳聚糖/明胶涂层，用于牙科植入物周围的软组织密封。

背景：牙科种植基台周围的软组织整合（STI）是防止细菌入侵和实现成功牙科种植康复的先决条件。然而，由于手术安装牙科种植体时免疫微环境的改变，种植体周围的 STI 是牙科基台安装后的一大挑战：方法：基于锌的已知免疫调节作用，我们在钛基底上沉积了锌/壳聚糖/明胶（Zn/CS/Gel）涂层，以研究其对巨噬细胞的影响。首先，我们将巨噬细胞置于含有不同浓度锌离子（Zn2+）的细胞培养基中。接着，我们探讨了通过简易电泳沉积（EPD）制备的锌/CS/凝胶涂层的免疫调节作用：结果：我们发现 Zn2+ 有效地改变了分泌组，减少了促炎症细胞因子的分泌，增强了促再生细胞因子的分泌，尤其是在 Zn2+ 补充量约为 37.5 μM 时。Zn/CS/Gel 涂层释放的 Zn2+ 浓度范围能有效刺激巨噬细胞的促再生极化，这一点已通过 M2 巨噬细胞极化得到证实。此外，这些暴露于 Zn2+ 的巨噬细胞对在条件培养基中培养的牙龈成纤维细胞的影响显示，它们的粘附、增殖和胶原分泌都受到了刺激：我们的研究结果表明，Zn/CS/凝胶涂层中 Zn2+ 的可控释放可用于种植体周围 STI 的免疫调节，并提高种植体的存活率。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Tissue engineering and regenerative medicine CELL & TISSUE ENGINEERING-ENGINEERING, BIOMEDICAL

CiteScore

6.80

自引率

5.60%

发文量

审稿时长

6-12 weeks

期刊介绍： Tissue Engineering and Regenerative Medicine (Tissue Eng Regen Med, TERM), the official journal of the Korean Tissue Engineering and Regenerative Medicine Society, is a publication dedicated to providing research- based solutions to issues related to human diseases. This journal publishes articles that report substantial information and original findings on tissue engineering, medical biomaterials, cells therapy, stem cell biology and regenerative medicine.